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The map label for this gene is acpT

Identifier: 187731194

GI number: 187731194

Start: 3573849

End: 3574418

Strand: Reverse

Name: acpT

Synonym: SbBS512_E3847

Alternate gene names: 187731194

Gene position: 3574418-3573849 (Counterclockwise)

Preceding gene: 187730420

Following gene: 187733742

Centisome position: 77.44

GC content: 56.32

Gene sequence:

>570_bases
ATGTATCGGATAGTTCTGGGGAAAGTTTCGACCTTAAGCGCAGCTCCACTGCCACCGGGTTTACGCGAGCAAGCACCGCA
AGGTCCACGACGCGAACGCTGGCTGGCGGGGCGTGCATTGCTTTCGCACACGCTTTCCCCGCTACCGGAGATCATCTATG
GCGAACAAGGCAAACCTGCATTCGCGCCGGAAACGCCGCTATGGTTCAACTTAAGCCATAGCGGTGACGATATCGCTCTG
CTGTTGAGCGATGAAGGCGAAGTCGGCTGCGATATCGAAGTGATTCGCCCGCGCGCCAACTGGCGCTGGCTGGCGAACGC
CGTGTTCAGCCTCGGGGAACACGCTGAAATGGACGCCGTGCATCCTGATCAGCAACTGGAAATGTTCTGGCGCATCTGGA
CGCGCAAAGAAGCCATCGTTAAACAGCGTGGCGGCAGCGCCTGGCAAATCGTCAGCGTAGATAGCACCTATCACTCCTCG
CTGTCAGTCAGCCATTGTCAGCTAGAAAATTTAAGCCTTGCGATCTGCACCCCCGTTACTTTATTCGTACCCCTTATAAT
GGGGTGTTAG

Upstream 100 bases:

>100_bases
GATTTTACTGGGGCCAGGCAGACCGAAAAGTCGGTTACCGGGATGAGGCGACAAGTGATACGATACGCACTTTCATTTTC
CATTAAACGTTGGCCCTGAT

Downstream 100 bases:

>100_bases
CCAGCCAGACCCGGCATGATTACTGCCCCCAGTCGTCCATGATCCGGGGGGGGATGTCACCGGGTCTGGTGGGGCGCTGG
TAACCGCTAATAGGGGTCAG

Product: holo-(acyl carrier protein) synthase 2

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPVTLFVPLIMGC

Sequences:

>Translated_189_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPVTLFVPLIMGC
>Mature_189_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPVTLFVPLIMGC

Specific function: May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Escherichia coli, GI1789886, Length=179, Percent_Identity=98.8826815642458, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 21036; Mature: 21036

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPVT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCEEEEHHHHH
LFVPLIMGC
HHHHHHHCC
>Mature Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPVT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCEEEEHHHHH
LFVPLIMGC
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]