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The map label for this gene is proW

Identifier: 187731166

GI number: 187731166

Start: 2986679

End: 2987743

Strand: Reverse

Name: proW

Synonym: SbBS512_E3201

Alternate gene names: 187731166

Gene position: 2987743-2986679 (Counterclockwise)

Preceding gene: 187731761

Following gene: 187733067

Centisome position: 64.73

GC content: 58.4

Gene sequence:

>1065_bases
ATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGTGCAATCCGCAGACGCCTGGGGTACACC
GGCGACTGCACCGACTGACGGCGGTGGTGCTGACTGGCTGACCAGTACGCCTGCGCCAAACGTCGAGCATTTTAATATTC
TCGATCCGTTCCATAAAACGCTGATCCCGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTTGTTACCCATTTCCGT
CCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTGGGTATGCCCGCACC
GGTGGCGATTATCGTTTTCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGCGTGGCGACGCTGGTTTCGCTGA
TTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCCATGGTTACCCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATC
GTCATTGGTTTGCCGCTGGGGATCTGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTCGTCCACTGCTTGATGCCAT
GCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGA
CGATCATCTTTGCTCTGCCGCCGATTATCCGTCTGACCATTCTGGGGATTAACCAGGTTCCGGCGGATCTGATTGAAGCC
TCGCGCTCATTCGGTGCCAGCCCGCGCCAGATGCTGTTCAAAGTTCAGTTACCGCTGGCGATGCCGACCATTATGGCGGG
CGTTAACCAGACGCTGATGCTGGCCCTTTCTATGGTGGTCATTGCCTCGATGATTGCCGTCGGCGGGCTGGGTCAGATGG
TACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTC
GATCGCCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCT
GCTGACCCGCCCATTCATTAAGTAA

Upstream 100 bases:

>100_bases
GCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTT
AGATCGTGAGGGGGTAAATA

Downstream 100 bases:

>100_bases
CTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGC
GACAGCGTTTGCCACGCTTA

Product: glycine betaine transporter membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFR
PVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIIL
DRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK

Sequences:

>Translated_354_residues
MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFR
PVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIIL
DRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
>Mature_353_residues
ADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRP
VFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIV
IGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEAS
RSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILD
RLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=354, Percent_Identity=99.4350282485876, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37618; Mature: 37487

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTPAPNVEHFNILDPFHKT
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIVIGLPLGIWLARSPRAAKII
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCH
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCHHHHCCCCCC
>Mature Secondary Structure 
ADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTPAPNVEHFNILDPFHKT
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIVIGLPLGIWLARSPRAAKII
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCH
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]