Click here to switch to the map view.

The map label for this gene is dusA

Identifier: 187731116

GI number: 187731116

Start: 4250832

End: 4251875

Strand: Reverse

Name: dusA

Synonym: SbBS512_E4546

Alternate gene names: 187731116

Gene position: 4251875-4250832 (Counterclockwise)

Preceding gene: 187731699

Following gene: 187731737

Centisome position: 92.11

GC content: 53.16

Gene sequence:

>1044_bases
TTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAG
TGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATA
CGTTGCTGTATACCGAAATGGTGACCACAGGGGCGATTATTCACGGTAAAGGTGATTACCTGGCGTACAGTGAAGAAGAA
CATCCGGTAGCGTTGCAACTCGGCGGTAGCGATCCGGCGGCGCTGGCACAGTGTGCGAAGCTGGCAGAAGCGCGTGGATA
TGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATG
CGCAGCTGGTTGCCGATTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATC
GATGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAACACCGTTTCCGGCAAAGGCGAGTGTGGGATGTTCATCAT
CCATGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGT
ATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACAC
CTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGAT
CTTTGGTTCCTCGGATACCGATGCCTATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCC
AGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTGTTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGT
TATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGTTGGAACACGCGCTCAAACTGGTGGCGGATAAGCG
TTAA

Upstream 100 bases:

>100_bases
GCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATAT
CGATAAAATAATCTCTACAT

Downstream 100 bases:

>100_bases
CTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATT
TTTGGCATGATTCTTGTAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR

Sequences:

>Translated_347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR
>Mature_347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=23.7012987012987, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=99.3939393939394, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=25.2100840336134, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.9387755102041, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38704; Mature: 38704

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEM
CCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEH
VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDR
HHHCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
VQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG
HCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGH
HHHHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
ITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEM
CCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEH
VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDR
HHHCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
VQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG
HCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPVAVVRAMYPYIERELSQGTYLGH
HHHHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
ITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]