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The map label for this gene is ycjW [H]

Identifier: 187730948

GI number: 187730948

Start: 1415480

End: 1416478

Strand: Reverse

Name: ycjW [H]

Synonym: SbBS512_E1553

Alternate gene names: 187730948

Gene position: 1416478-1415480 (Counterclockwise)

Preceding gene: 187733989

Following gene: 187731184

Centisome position: 30.69

GC content: 45.35

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
TAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAGCCAAACAAGCTGGCCCGCG
CGCTGACCTCTTCGGGCTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCTGAAGTTTTGCATGCCATCACCGCCAAAGCTGAAGAGGAAGGTTTCGACGTGATATTGCAGACGTCGCACAATCCGGC
AGAAGACTTACAAAAATGTGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTAAGTTCTCCAGCGGATG
AGTCATTTTTTGCCCAACTTGATAAATACGATATTCCTGTCGTGGTGATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGCGACAGCATTGCGTTGACCGATACATTAATTGAAAGTGGGCATCAAAATATTGC
CTGCCTGCATGCTCCGCTTGATGTCCATGTTTCAGTGGATCGGGTAAATGGTTATAAGCAGAGCCTGGCTGCGCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGACGGTGGTTATACCCATGAAACAGCACTGCAAGCCGCGAGAGAATTATTAAGC
CAGTCACCGTTGCCTGAGGCGGTGTTTGCCACTGACAGTCTGAAATTAATGAGTATTTATCGTGCGGCGGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGTAATGAAACGCTGTCGTTTATTTTAACACCTGCACCGGGCG
GCATCGATGTTCCGACGCAGGAGTTAGGTCAACAAAGCTGCGAGTTATTATTCCGCTTAATTTCTGGAAAACCGTCACCA
CAGAATATTACCGTTGCCACGCATATGACGCTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTGCCCGTGTTGTCATCGACGGGCGAACTCCATAAGATGTGTAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATACCAGCTACCTTGCCTGAATGCCGGATGCGATGCTAGCGCATCTTATCCGGCCTACATTTATAGATCGGATAAAGTAC
TCGCGCCGCATCCGGCAATT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDTLIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDTLIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSP
QNITVATHMTLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDTLIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQ
NITVATHMTLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=99.0963855421687, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.5555555555556, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=30.0940438871473, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.1021671826625, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=26.8987341772152, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=29.0625, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=24.9211356466877, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=25.3048780487805, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=27.8787878787879, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=21.5625, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36166; Mature: 36034

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDTLIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDTLIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARELLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLISGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]