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The map label for this gene is rumB

Identifier: 187730916

GI number: 187730916

Start: 2274850

End: 2275977

Strand: Reverse

Name: rumB

Synonym: SbBS512_E2472

Alternate gene names: 187730916

Gene position: 2275977-2274850 (Counterclockwise)

Preceding gene: 187731461

Following gene: 187731722

Centisome position: 49.31

GC content: 54.26

Gene sequence:

>1128_bases
ATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAACGCAGCTGATTCCAGAGCAACTCTCCGC
TAAAACCGCCGATCTTAAAAATCTGCTCGCCGATTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAG
GGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTATGCTGCATCGCGATGGCACG
CCGGAAGACCTTTGTGACTGCCCGCTTTATCCTGCCTCTTTTGCGCCCGTTTTTGCGGCGCTAAAACCGTTTATCGCCTG
TGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGCGATG
GCGGCATGATGCTGCGCTTTGTACTGCGTTCTGAAACCAAACTGGCACAACTGCGTAAGGCGCTGCCGTGGTTACAGGAA
CAACTACCGCAACTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTA
CCTGACTGAACAACAGGCGCTGGCAGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTA
ATCCGGCGGTCGCCAGCCAGCTGTACGCTACCGTGCGCGACTGGGTACGGCAGCTGCCAGTTAAACATATGTGGGATCTG
TTCTGCGGTGTGGGGGGCTTTGGTTTACACTGCGCGACGCCTGACATGCAGTTAACCGGGATCGAAATTGCGCCAGAGGC
CATTGCCTGTGCAAAGCAGTCAGCCGCTGAACTGGGCTTAACTCGTTTACAATTTCAGGCGCTGGACTCCACTCAGTTTG
CCACTGCTCAGGGGGAGGTGCAGGAGTTGGTGCTGGTTAACCCGCCGCGCCGCGGCATTGGTAAACCGCTGTGTGATTAT
CTCTCAACGATGGCACCGCGTTTTATCATCTACTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCC
TGGGTTTCGCATCGAACGGGTACAGCTTTTTGATATGTTCCCGCACACCGCGCACTATGAAGTGCTGACGCTGCTGGTGA
AGCAATAA

Upstream 100 bases:

>100_bases
CTCATCCTGATGCTGCTGTTCGTTCCCTCGACCAGTCGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCT
GATGATGTGAAGGTCAATGT

Downstream 100 bases:

>100_bases
AAAAGCCGCAGGTGCGGCTTCAGATTGCTGATCAGGTGCGCTTTGTTCATGCCGGATGCGGCGTGTACGCCTTATCCGGC
CTACAAAATCATGCTAAATC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQCALYDAGRCRSCQWITQLIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIACAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATVRDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVQELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ

Sequences:

>Translated_375_residues
MQCALYDAGRCRSCQWITQLIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIACAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATVRDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVQELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ
>Mature_375_residues
MQCALYDAGRCRSCQWITQLIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIACAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATVRDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVQELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI51173878, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=97.6, Blast_Score=756, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=377, Percent_Identity=27.0557029177719, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.1208791208791, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666579, Length=360, Percent_Identity=23.8888888888889, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41977; Mature: 41977

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQLIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIACAGLTPYNVARKRG
EEEECCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATVRDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCHHCCCCCCC
QELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMF
EEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEHEEEEHHC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQLIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIACAGLTPYNVARKRG
EEEECCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATVRDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCHHCCCCCCC
QELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGFRIERVQLFDMF
EEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEHEEEEHHC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA