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The map label for this gene is cysN

Identifier: 187730850

GI number: 187730850

Start: 2916856

End: 2918283

Strand: Direct

Name: cysN

Synonym: SbBS512_E3123

Alternate gene names: 187730850

Gene position: 2916856-2918283 (Clockwise)

Preceding gene: 187731887

Following gene: 187731484

Centisome position: 63.19

GC content: 54.69

Gene sequence:

>1428_bases
ATGAACACCGCACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGCCTGCTGCACGATACCCGCCAAA
TCTACGAAGATCAGCTCTCATCGCTGCATAACGACAGTAAGCGTCACGGCACCCAGGGCGAAAAGCTGGATCTGGCACTG
CTGGTGGACGGCCTGCAAGCTGAGCGCGAACAGGGCATCACCATTGACGTGGCTTACCGTTATTTCTCTACCGAGAAGCG
TAAATTTATTATCGCCGACACCCCAGGGCACGAGCAGTACACTCGCAATATGGCGACTGGCGCATCGACATGTGAACTGG
CGATCTTACTGATCGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACACTGTTGGGGATC
AAACATCTGGTCGTGGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACGTTCACCCGTATTCGTGAAGATTA
TTTGACCTTTGCCGGGCAGCTGCCGGGTAATCTGGATATCCGCTTTGTGCCGCTCTCTGCACTGGAAGGCGACAACGTGG
CATCGCAAAGTGAAAGTATGTCGTGGTACAGCGGTCCGACACTGCTCGAAGTGCTGGAAACCGTGGAGATCCAGCGAGTG
GTGGATGCCCAGCCAATGCGCTTCCCGGTGCAGTACGTTAATCGCCCGAATCTCGATTTTCGTGGCTACGCCGGAACGCT
GGCATCCGGTCGCGTGGAAGTCGGGCAACGTGTCAAAGTGCTGCCCTCTGGTGTGGAATCAAACGTCGCGCGGATCGTGA
CTTTTGATGGTGATCGCGAAGAAGCCTTTGCCGGAGAAGCGATCACCCTGGTGCTGACGGATGAGATCGACATCAGCCGT
GGCGATCTGCTGCTGGCGGCAGACGAAGCGTTACCGGCGGTGCAGAGCGCGTCGGTGGATGTGGTATGGATGGCGGAACA
GCCGCTTTCCCCGGGCCAGAGTTACGACATCAAAATTGCCGGTAAGAAGACGCGCGCGCGTGTTGATGGCATTCGCTATC
AGGTTGATATTAATAACCTTACCCAGCGTGAAGTTGAAAACCTGCCACTGAATGGGATCGGCCTCGTGGATCTCACTTTT
GACGAGCCGCTGGTGTTAGATCGTTATCAACAAAATCCGGTGACGGGTGGGCTGATTTTTATCGATCGCCTGAGCAACGT
GACTGTTGGTGCCGGTATGGTGCATGAGCCGAAAAACGAGGCCGCCGTTGCGTCATCTGAATTCAGTGCATTCGAACTGG
AATTGAATGCCCTGGTTCGTCGCCACTTTCCGCACTGGGGCGCGCGCGATTTGCTGGGGGATAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCTACCACCAGTGAACGTCAGGACCGCGTGATTGACCGCGACCAGGCGGGGTCTATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGCCATCCGGTCACTGTGCAACAACGCGAGCTACACCACTGTCATCGT
GGTGTAGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK

Sequences:

>Translated_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
>Mature_475_residues
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=28.8329519450801, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=28.8329519450801, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=33.7883959044369, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=33.7883959044369, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=33.7883959044369, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.5395033860045, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=26.530612244898, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=32.7645051194539, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI34147630, Length=302, Percent_Identity=29.4701986754967, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157426893, Length=351, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=98.5263157894737, Blast_Score=958, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=326, Percent_Identity=26.9938650306748, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789737, Length=326, Percent_Identity=26.9938650306748, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367247, Length=293, Percent_Identity=26.6211604095563, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115532067, Length=342, Percent_Identity=29.5321637426901, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=342, Percent_Identity=29.5321637426901, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.4037558685446, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=28.9887640449438, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=28.9887640449438, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.6255707762557, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=26.9709543568465, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=28.735632183908, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=393, Percent_Identity=30.0254452926209, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=27.9176201372998, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=149, Percent_Identity=34.2281879194631, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=28.5388127853881, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45553807, Length=293, Percent_Identity=32.4232081911263, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553816, Length=293, Percent_Identity=32.4232081911263, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651721, Length=293, Percent_Identity=32.4232081911263, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17864154, Length=293, Percent_Identity=32.4232081911263, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=26.0770975056689, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=26.0770975056689, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137380, Length=315, Percent_Identity=30.4761904761905, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281363316, Length=316, Percent_Identity=29.746835443038, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17864358, Length=316, Percent_Identity=29.746835443038, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922690, Length=289, Percent_Identity=25.9515570934256, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52594; Mature: 52594

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCCEECCCCCHHEECCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMSWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPKNEAAVASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCCEECCCCCHHEECCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA