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The map label for this gene is nrdE

Identifier: 187730558

GI number: 187730558

Start: 2990262

End: 2992406

Strand: Reverse

Name: nrdE

Synonym: SbBS512_E3204

Alternate gene names: 187730558

Gene position: 2992406-2990262 (Counterclockwise)

Preceding gene: 187732904

Following gene: 187734239

Centisome position: 64.83

GC content: 52.49

Gene sequence:

>2145_bases
TTGGCAACGACAACCGCAGAATGCCTGACGCAGGAAACGATGGATTACCACGCGCTGAATGCGATGCTTAACCTCTACGA
TAGCGCAGGTCGCATTCAGTTCGATAAAGACCGCCAGGCCGTTGACGCCTTTATGGCGACGCATGTGCGTCCGAACAGTG
TGACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTAC
TCTCGCGACTTTGTCATTACGCTGTTTGCCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAA
GTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTCACGATGGTGG
CGCTGACGCTGGCACAAGGCGATGCGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCC
ACGCCGACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTCTGCTGCGTATTGAAGACAATAT
GGAGTCGATTGGCCGGGCGGTAAATTCCGCACTGCAACTGTCGAAACGCGGCGGCGGCGTGGCATTTTTGCTGTCGAATC
TGCGAGAAGCGGGCGCGCCAATTAAACGTATTGAAAATCAATCTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGAC
GCATTTTCCTATGCCAACCAACTCGGCGCGCGTCAGGGGGCAGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCT
GCGTTTTCTCGATACAAAACGGGAAAATGCCGACGAAAAAATCCGCATTAAAACACTGTCGCTGGGCGTGGTGATCCCGG
ATATCACTTTCCATCTGGCAAAAGAGAATGCGCAGATGGCGCTGTTTTCGCCCTATGACGTTGAGCGAGTTTATGGCAAG
CCGTTTGCCGATATCGCCATCAGCGAACACTATGACGAACTGGTTGCCGATGAACGCATCCGCAAAAAATACCTCAACGC
CCGTGATTTCTTCCAGCGGCTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACC
GTGCTAACCCTGTCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAGATTTTGCAGGTCAACTGTGCCTCAGAGTAT
GACGAGAATCTCGACTATGCCCGCACAGGCCATGATATCTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGA
TTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCGGTGTCAGATATGAGTCATATCCGCAGCG
TGCCGTCCATCGAAGCCGGAAATGCCGCCTCACACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGA
GAAGGCATCGCTTATGGTTCGCCGGAAGCACTGGATTTCACCAATCTCTATTTCTATGCCATCACCTGGCACGCACTGCG
TACCTCGATGTTGCTGGCGCGCGAACGCGGTGAAACCTTCGCCGGGTTCAAACAGTCACGTTATGCCAGTGGTGAATATT
TTAGCCAATATCTGCAAGGTAACTGGCAGCCGAAAACGGCGAAAGTTGGCGAACTGTTTGCCCGCAGCGGTATTACGTTA
CCTACCCGTGAGATGTGGGCGCAACTGCGCGACGACGTGATGCGCTACGGCATTTATAACCAGAATCTTCAGGCGGTGCC
GCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAG
AGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGATGCTTACGAA
ATCGGCGCAGAAAAGATCATCGACACCTACGCCGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTGTTTTT
CCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACA
TTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCTGTGCACTTTAA

Upstream 100 bases:

>100_bases
ATGTGATTGCCCGGAAATGCGGCGTGCCGTGGCTGTACCGTTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTT
CGTAAAGGAGTAACCGAATT

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
TCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Sequences:

>Translated_714_residues
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
>Mature_713_residues
ATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYS
RDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPAT
PTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDA
FSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKP
FADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYD
ENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLP
TREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEI
GAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=669, Percent_Identity=27.2047832585949, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=98.7394957983193, Blast_Score=1480, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.9450171821306, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=748, Percent_Identity=25, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=620, Percent_Identity=26.1290322580645, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=25.8164852255054, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17352481, Length=616, Percent_Identity=24.025974025974, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80367; Mature: 80236

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHEEHHEEEECCCEEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
ATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHEEHHEEEECCCEEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]