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The map label for this gene is rbsR

Identifier: 187730470

GI number: 187730470

Start: 3889699

End: 3890691

Strand: Reverse

Name: rbsR

Synonym: SbBS512_E4165

Alternate gene names: 187730470

Gene position: 3890691-3889699 (Counterclockwise)

Preceding gene: 187732009

Following gene: 187732811

Centisome position: 84.29

GC content: 50.55

Gene sequence:

>993_bases
TTGGCTACAATGAAAGATGTTTCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTT
CGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCCCTGGCGCGTAGCC
TCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGC
GTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAA
TCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCA
TGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAAC
TCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGTCCGCT
GGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCT
ATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCCATGCGCCAACTGTTATCACATCCGCTGCGTCCTCAG
GCCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGA
TATCGCGGTGATTGGCTATGACGATATCGAACTGGCAAGCTTTATGACGCCACCATTAACCACTATCCACCAACCGAAAG
ATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTT
ACTCCGATTCTGATGGAACGCGGTTCGGCTTAG

Upstream 100 bases:

>100_bases
CCATGCTGCAGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGTATTTT
TAGACAGGCAGAGGTGACGC

Downstream 100 bases:

>100_bases
ATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATA
ATGCCCATCGTGATAAACGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATMKDVSRLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQL
TPILMERGSA

Sequences:

>Translated_330_residues
MATMKDVSRLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQL
TPILMERGSA
>Mature_329_residues
ATMKDVSRLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQA
VFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLT
PILMERGSA

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=99.6969696969697, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=46.6666666666667, Blast_Score=320, Evalue=6e-89,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.2750809061489, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.1058020477816, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=30.0940438871473, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.2258064516129, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=29.1803278688525, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=27.3615635179153, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=28.3536585365854, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=25.5384615384615, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=25.974025974026, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=26.4984227129338, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790192, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=23.8532110091743, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36628; Mature: 36497

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVSRLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPTLQQQRLQLTPILMERGSA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATMKDVSRLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPTLQQQRLQLTPILMERGSA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]