Click here to switch to the map view.

The map label for this gene is thiC

Identifier: 187730178

GI number: 187730178

Start: 4195060

End: 4196955

Strand: Reverse

Name: thiC

Synonym: SbBS512_E4485

Alternate gene names: 187730178

Gene position: 4196955-4195060 (Counterclockwise)

Preceding gene: 187730310

Following gene: 187730775

Centisome position: 90.92

GC content: 55.54

Gene sequence:

>1896_bases
ATGTCTGCAACAAAACTGACCCGCCGCGAACAACGCGCCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTT
TCCCAACTCAAAACGCATTTACCTCACTGGCACACACTCTGGCGTTCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGA
CGCTAATCGGCGGCAGCAAAGAACAGCCGCAGTTTGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCATAT
GGCGATCCGCAGATCGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATAC
CGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCG
GCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACACCG
GAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATACGTAGCGAAGTTTTACGCCACCAGCATCCGGG
AATGAGCTTTGGCGCACGTCTGCCGGAAAATATCACTGCGGAATTTGTCCGTGATGAAGTTGCTGCCGGACGTGCGATTA
TCCCGGCCAACATTAATCATCCGGAATCGGAGCCGATGATTATTGGCCGTAACTTCCTGGTAAAAGTTAACGCCAATATC
GGCAACTCGGCGGTCACCTCTTCCATCGAAGAAGAAGTGGAAAAGCTGGTATGGTCCATGCGCTGGGGAGCGGATACGGT
GATGGATCTCTCCACCGGTCGCTATATTCACGAAACCCGCGAGTGGATTTTGCGTAACAGTCCGGTGCCGATCGGTACAG
TGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCCGCGACACGCTGCTG
GAACAGGCCGAGCAAGGTGTGGATTACTTCACTATCCATGCGGGCGTACTGTTGCGCTATGTGCCGATGACCGCGAAACG
CCTGACCGGTATCGTCTCTCGCGGCGGTTCGATTATGGCGAAATGGTGCCTCTCCCATCATCAGGAAAATTTCCTCTATC
AACACTTCCGCGAAATTTGTGAAATCTGTGCCGCTTATGACGTTTCGCTGTCGCTGGGCGACGGTCTGCGCCCCGGTTCT
ATTCAGGACGCCAACGATGAAGCGCAGTTTGCCGAGCTGCATACGCTGGGCGAACTGACCAAAATTGCCTGGGAATATGA
CGTGCAGGTGATGATTGAAGGCCCAGGCCACGTGCCGATGCAGATGATCCGCCGCAATATGACCGAGGAGTTAGAGCACT
GCCACGAAGCGCCGTTTTACACTCTGGGGCCGCTAACTACCGATATTGCGCCGGGCTATGACCACTTCACGTCGGGGATT
GGTGCGTCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCTAATAA
AGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCCGCCCACGCCGCTGACCTGGCGAAAGGGCATCCGGGCGCGC
AAATTCGCGATAACGCCATGTCGAAAGCCCGCTTCGAATTTCGCTGGGAAGACCAGTTTAATCTGGCCCTCGACCCGTTT
ACCGCCCGCGCTTATCACGATGAAACCCTGCCGCAAGAGTCAGGTAAAGTCGCCCATTTTTGCTCCATGTGTGGGCCGAA
ATTCTGCTCGATGAAAATCAGCCAGGAAGTGCGTGATTACGCCGCCACGCAAACTATTGAAATGGGAATGGCGGATATGT
CGGAGAACTTCCGTGCCAGAGGCGGAGAAATCTACCTGCGTAAGGAGGAAGCGTGA

Upstream 100 bases:

>100_bases
GGAACAAGAGTTAATCTGCTATCGCATCGCCTTTGCGGCGATCGTCTCTTGCTTCATCCGTCGTCTGACAAGCCACGTCC
TTAACTTTTTGGAATGAGCT

Downstream 100 bases:

>100_bases
TGTATCAGCCTGATTTTCCTCCTGTACCTTTTCGTTTAGGACTGTACCCAGTGGTGGACAGCGTACAGTGGATCGAACGT
CTGTTGGATGCAGGCGTACG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRVPMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPY
GDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSMRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GASMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEIYLRKEEA

Sequences:

>Translated_631_residues
MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRVPMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPY
GDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSMRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GASMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEIYLRKEEA
>Mature_630_residues
SATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRVPMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYG
DPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITPE
MEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIG
NSAVTSSIEEEVEKLVWSMRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLE
QAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSI
QDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGIG
ASMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFT
ARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEIYLRKEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=631, Percent_Identity=99.0491283676704, Blast_Score=1308, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70890; Mature: 70759

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRVPMREIQLSPTLIGGSK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEECCCC
EQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKA
CCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
RLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEEECCC
GNSAVTSSIEEEVEKLVWSMRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCC
GASMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AATQTIEMGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
SATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRVPMREIQLSPTLIGGSK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHEEECCEEECCCC
EQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWIDARGDTEELTVRSSDYTKA
CCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
RLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECEEEEEEECCC
GNSAVTSSIEEEVEKLVWSMRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCC
GASMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
HHCEEEEEECCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AATQTIEMGMADMSENFRARGGEIYLRKEEA
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA