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The map label for this gene is btuE

Identifier: 187730047

GI number: 187730047

Start: 1756526

End: 1757077

Strand: Reverse

Name: btuE

Synonym: SbBS512_E1914

Alternate gene names: 187730047

Gene position: 1757077-1756526 (Counterclockwise)

Preceding gene: 187731832

Following gene: 187730625

Centisome position: 38.06

GC content: 49.82

Gene sequence:

>552_bases
ATGCAAGATTCCATTCTGACGACCGTAGTGAAAGATATCGACGGTGAAGTGACCACGCTGGAGAAGTACGCCGGTAATGT
GCTGTTGATTGTCAATGTCGCCTCAAAGTGTGGCTTAACGCCGCAATATGAGCAGTTGGAGAATATTCAGAAAGCCTGGG
CCGATCGAGGTTTTGTGGTGCTGGGATTCCCGTGCAACCAGTTTCTGGAACAAGAACCGGGCAGCGATGAAGAGATTAAA
ACTTACTGTACCACCACATGGGGGGGGACGTTCCCGATGTTCAGTAAGATTGAAGTTAACGGCGAAGGACGCCATCCGCT
GTATCAAAAATTGATTGCCGCAGCGCCGACCGCAGTCGCGCCGGAAGAGAGCGGATTCTATGCCCGTATGGTCAGCAAAG
GTCGTGCACCGCTGTACCCTGATGATATTTTATGGAATTTTGAGAAATTTTTAGTTGGCAGGGACGGATTGGTCATCCAG
CGTTTTTCCCCGGATATGACGCCGGAAGACCCCATTGTGATGGAAAGCATTAAACTGGCGTTGGCAAAATAA

Upstream 100 bases:

>100_bases
CGGTGTTTATCTGGTTATTGTTAAAAGCAAGACGTTAGCCGCAAAAAGACGGTCTATGATTAAAAGCTAAGATTTTTACT
GACCACACCCAGGAGAAACG

Downstream 100 bases:

>100_bases
TGTCTATTGTGATGCAGTTACAGGATGTTGCGGAATCTACCCGCCTGGGGCCACTTTCTGGCGAGGTTCGGGCTGGGGAG
ATCCTGCACCTGGTGGGGCC

Product: putative glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYEQLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIK
TYCTTTWGGTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
RFSPDMTPEDPIVMESIKLALAK

Sequences:

>Translated_183_residues
MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYEQLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIK
TYCTTTWGGTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
RFSPDMTPEDPIVMESIKLALAK
>Mature_183_residues
MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYEQLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIK
TYCTTTWGGTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
RFSPDMTPEDPIVMESIKLALAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=167, Percent_Identity=40.1197604790419, Blast_Score=117, Evalue=4e-27,
Organism=Homo sapiens, GI90903240, Length=173, Percent_Identity=39.3063583815029, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI15618997, Length=160, Percent_Identity=39.375, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI4557629, Length=157, Percent_Identity=39.4904458598726, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI41406084, Length=155, Percent_Identity=41.9354838709677, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI6006001, Length=161, Percent_Identity=43.4782608695652, Blast_Score=102, Evalue=1e-22,
Organism=Homo sapiens, GI90903238, Length=148, Percent_Identity=39.8648648648649, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI192455698, Length=171, Percent_Identity=33.9181286549708, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI33186887, Length=151, Percent_Identity=36.4238410596026, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI32967607, Length=159, Percent_Identity=37.1069182389937, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=97.2677595628415, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535473, Length=168, Percent_Identity=41.6666666666667, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17506887, Length=180, Percent_Identity=38.8888888888889, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71993584, Length=168, Percent_Identity=35.1190476190476, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17550320, Length=187, Percent_Identity=34.7593582887701, Blast_Score=109, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI193209493, Length=187, Percent_Identity=32.620320855615, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193210707, Length=167, Percent_Identity=35.3293413173653, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71993573, Length=95, Percent_Identity=35.7894736842105, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71999642, Length=76, Percent_Identity=38.1578947368421, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=168, Percent_Identity=45.8333333333333, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322228, Length=157, Percent_Identity=44.5859872611465, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322826, Length=166, Percent_Identity=37.3493975903614, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24656772, Length=169, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24656777, Length=169, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24656767, Length=169, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI85726443, Length=182, Percent_Identity=34.0659340659341, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20369; Mature: 20369

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1 ; PS00763 GLUTATHIONE_PEROXID_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYEQLENIQKAWADRGFVV
CCCHHHHHHHHHCCCCEEEHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEE
LGFPCNQFLEQEPGSDEEIKTYCTTTWGGTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVA
EECCHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
PEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQRFSPDMTPEDPIVMESIKLA
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LAK
HCC
>Mature Secondary Structure
MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYEQLENIQKAWADRGFVV
CCCHHHHHHHHHCCCCEEEHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCEEE
LGFPCNQFLEQEPGSDEEIKTYCTTTWGGTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVA
EECCHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
PEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQRFSPDMTPEDPIVMESIKLA
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LAK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]