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The map label for this gene is nedA [H]

Identifier: 187735131

GI number: 187735131

Start: 733948

End: 735207

Strand: Reverse

Name: nedA [H]

Synonym: Amuc_0625

Alternate gene names: 187735131

Gene position: 735207-733948 (Counterclockwise)

Preceding gene: 187735138

Following gene: 187735127

Centisome position: 27.6

GC content: 57.22

Gene sequence:

>1260_bases
ATGACATGGTTGTTGTGCGGCAGGGGAAAATGGAATAAGGTGAAGAGGATGATGAATTCCGTATTCAAGTGTTTGATGAG
TGCCGTATGCGCCGTGGCATTGCCGGCGTTCGGGCAGGAAGAGAAAACCGGTTTCCCTACGGACAGGGCTGTGACCGTAT
TCAGCGCCGGGGAGGGTAATCCCTATGCGTCCATCCGTATTCCCGCCCTGCTCAGTATCGGCAAGGGCCAGCTTCTGGCA
TTCGCCGAAGGACGGTACAAAAATACCGACCAGGGGGAGAACGATATTATCATGAGCGTCAGCAAGAATGGCGGGAAGAC
CTGGTCCCGTCCCCGGGCGATAGCCAAGGCCCATGGCGCCACGTTCAATAATCCGTGCCCCGTTTATGATGCCAAAACCA
GGACCGTGACTGTCGTATTCCAGCGTTACCCTGCCGGGGTCAAGGAGCGGCAGCCCAATATCCCGGACGGATGGGATGAT
GAAAAGTGCATCCGCAATTTCATGATTCAGAGCAGGAACGGAGGTTCTTCCTGGACGAAGCCGCAGGAGATCACGAAGAC
GACCAAGCGTCCTTCCGGAGTGGATATTATGGCGTCCGGCCCGAATGCGGGAACCCAGCTGAAGAGCGGCGCCCACAAGG
GCCGCCTGGTGATTCCGATGAATGAAGGGCCGTTCGGCAAATGGGTGATTTCCTGCATTTACAGCGATGACGGCGGCAAG
AGCTGGAAGCTGGGCCAGCCGACTGCCAATATGAAGGGCATGGTGAACGAGACGTCCATTGCGGAAACGGATAACGGCGG
CGTTGTGATGGTTGCGCGCCATTGGGGCGCAGGCAATTGCCGCCGTATTGCGTGGTCCCAGGATGGCGGGGAGACCTGGG
GACAGGTGGAGGACGCTCCGGAGCTGTTTTGCGACAGTACCCAGAATTCCCTGATGACGTATTCCCTGAGCGACCAGCCT
GCCTATGGCGGCAAAAGCCGCATTCTGTTTTCCGGGCCCAGTGCGGGCCGGCGCATTAAGGGACAGGTGGCCATGAGCTA
TGACAACGGCAAGACCTGGCCGGTGAAGAAATTGCTGGGCGAGGGCGGTTTTGCCTATTCCAGCCTTGCCATGGTGGAAC
CCGGCATCGTTGGGGTGCTTTATGAGGAGAACCAGGAGCATATTAAAAAGCTGAAGTTTGTTCCCATTACCATGGAATGG
CTGACGGACGGAGAAGACACAGGGCTGGCTCCCGGCAAAAAAGCTCCTGTTCTCAAGTAG

Upstream 100 bases:

>100_bases
ATTTTGGGCCGAAGCCTTCCGGGCTGCCCGGCGGCGGTATCGCGTTCCTGCCGCGCGTTCCTCTTTAGTGTTCCTTTTTC
TCTGGTCTTGGCGCACATGC

Downstream 100 bases:

>100_bases
AGGAAAGCCGGCTAATACGTTTTTCCCTTCCGGAGGTGTCTCCGGGAGGGGGAGATTGAATGCTTTCCTCCGTAGCGGAG
CGTTATGGAAGAAGGCAGAT

Product: Exo-alpha-sialidase

Products: NA

Alternate protein names: Neuraminidase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTWLLCGRGKWNKVKRMMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGNPYASIRIPALLSIGKGQLLA
FAEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAHGATFNNPCPVYDAKTRTVTVVFQRYPAGVKERQPNIPDGWDD
EKCIRNFMIQSRNGGSSWTKPQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCIYSDDGGK
SWKLGQPTANMKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSQDGGETWGQVEDAPELFCDSTQNSLMTYSLSDQP
AYGGKSRILFSGPSAGRRIKGQVAMSYDNGKTWPVKKLLGEGGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITMEW
LTDGEDTGLAPGKKAPVLK

Sequences:

>Translated_419_residues
MTWLLCGRGKWNKVKRMMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGNPYASIRIPALLSIGKGQLLA
FAEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAHGATFNNPCPVYDAKTRTVTVVFQRYPAGVKERQPNIPDGWDD
EKCIRNFMIQSRNGGSSWTKPQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCIYSDDGGK
SWKLGQPTANMKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSQDGGETWGQVEDAPELFCDSTQNSLMTYSLSDQP
AYGGKSRILFSGPSAGRRIKGQVAMSYDNGKTWPVKKLLGEGGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITMEW
LTDGEDTGLAPGKKAPVLK
>Mature_418_residues
TWLLCGRGKWNKVKRMMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGNPYASIRIPALLSIGKGQLLAF
AEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAHGATFNNPCPVYDAKTRTVTVVFQRYPAGVKERQPNIPDGWDDE
KCIRNFMIQSRNGGSSWTKPQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCIYSDDGGKS
WKLGQPTANMKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSQDGGETWGQVEDAPELFCDSTQNSLMTYSLSDQPA
YGGKSRILFSGPSAGRRIKGQVAMSYDNGKTWPVKKLLGEGGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITMEWL
TDGEDTGLAPGKKAPVLK

Specific function: To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors [H]

COG id: COG4409

COG function: function code G; Neuraminidase (sialidase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 F5/8 type C domain [H]

Homologues:

Organism=Homo sapiens, GI4557791, Length=359, Percent_Identity=26.4623955431755, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI117190519, Length=453, Percent_Identity=25.3863134657837, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI222352170, Length=396, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018905
- InterPro:   IPR002860
- InterPro:   IPR000421
- InterPro:   IPR008979
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR011040
- InterPro:   IPR006311 [H]

Pfam domain/function: PF02012 BNR; PF00754 F5_F8_type_C; PF10633 NPCBM_assoc [H]

EC number: =3.2.1.18 [H]

Molecular weight: Translated: 45590; Mature: 45458

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLLCGRGKWNKVKRMMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGN
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
PYASIRIPALLSIGKGQLLAFAEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAHGA
CEEEEEECEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCC
TFNNPCPVYDAKTRTVTVVFQRYPAGVKERQPNIPDGWDDEKCIRNFMIQSRNGGSSWTK
CCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHEECCCCCCCCCC
PQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCIYSDDGGK
CHHHHHHCCCCCCCEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCC
SWKLGQPTANMKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSQDGGETWGQVEDAP
EEECCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCH
ELFCDSTQNSLMTYSLSDQPAYGGKSRILFSGPSAGRRIKGQVAMSYDNGKTWPVKKLLG
HHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHC
EGGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITMEWLTDGEDTGLAPGKKAPVLK
CCCCCEEEHEEECCCEEEEEECCCHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TWLLCGRGKWNKVKRMMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGN
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
PYASIRIPALLSIGKGQLLAFAEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAHGA
CEEEEEECEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCC
TFNNPCPVYDAKTRTVTVVFQRYPAGVKERQPNIPDGWDDEKCIRNFMIQSRNGGSSWTK
CCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHEECCCCCCCCCC
PQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCIYSDDGGK
CHHHHHHCCCCCCCEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCC
SWKLGQPTANMKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSQDGGETWGQVEDAP
EEECCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCH
ELFCDSTQNSLMTYSLSDQPAYGGKSRILFSGPSAGRRIKGQVAMSYDNGKTWPVKKLLG
HHHHCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHC
EGGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITMEWLTDGEDTGLAPGKKAPVLK
CCCCCEEEHEEECCCEEEEEECCCHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400240; 8591030 [H]