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The map label for this gene is nadE [H]

Identifier: 187735126

GI number: 187735126

Start: 727943

End: 729877

Strand: Direct

Name: nadE [H]

Synonym: Amuc_0620

Alternate gene names: 187735126

Gene position: 727943-729877 (Clockwise)

Preceding gene: 187735123

Following gene: 187735128

Centisome position: 27.32

GC content: 58.97

Gene sequence:

>1935_bases
ATGTTCGGATATTACAGACTTGCCTCCGCCGTCCCCCAGCTCCGCGTGGCTGAAGTGGACTACAATGTCGACCAACTTAC
GGAGGGATTCCGGAAAGCCGCAGAACAGCAGGCGGCGGCCGTCGTTTTCCCGGAACTGTGCATTACGGGCTATTCCTGCG
GAGACCTTTTCTTCCAGCCGAACCTGAGGAAAGCAGCCCTGAACGGCCTGCTCCGCTTTACGGAAGCCACGGAAGGCTCC
GGCACGATTGCCGTGGTGGGCCTGCCCTTCCTTCATGAAGACGCTCTGTATAATACGGCGGCGGTAGTGCAGTCCGGCCG
CATTCTGGCTCTGGTGCCCAAAACCGTGCTCCCCAACTACCGGGAATTCTACGAAAAGCGGCAATTCACCTCCGGCAGGG
AATTGGGTACAGGAGTGAAGGAAGTCACAGTCAATGGGGTGCATATCCCGTTCGGGACGGAAATCGTTTTTCATGAAGAA
TCGTCTCCCTTCAGTTTTGGCGTGGAAATATGTGAAGACCTGTGGAGCGTCATTCCTCCCAGTTCCAAACTGGCTCTTCT
GGGTGCCAGAGCCATTCTCAACCCTTCCGCAGGAACGGAACTCACCGGTAAGGCCGCATACAGGAGGGAACTGGTCAGAC
AGCAGAGCGGCAGATGCCTCTGCGCCTATGTCCTTTCCTCCGCAGGTGTGCATGAATCCACCACAGACACCGTATTCGGC
GGCCATTCCCTCATTGCGGACAACGGCAGGCCAGCAGCGGAGGGGGAACGGTTCTGCAGGGAAAGCACCCTCATCTTTGC
GGACGTGGATTTTGAACGGCTGGAGGCGGCGCGCCTCTCTGAAAGCTCCTTCAATGACAGCAAATCCCTTTTCCCTGCCG
GGAATGCCCTGCATCTGGCCCTGCCGGAACAGGTTCCCGGAGCGCCCGGCCTGGAATACGCTTTCAACCCGGCACTGCCC
TTTCTGCCTTCTCCGTCCCGCCGCCGGGAACGGTGTGAAGAAATCATCTCCATCCAAACGGCAGGTCTGGCCAAAAGAAT
GGAACATACCCGCGCGCAACGGCTCGTCATCGGCATCTCCGGCGGGCTGGACTCCACGCTGGCTCTGCTCATCTGCTCCC
GCGCCTGCCGTGCGCTGAAGCGCCCTGCCTCGGACATTCTGGCCGTGACAATGCCCGGTTTCGGCACCACGGACAGAACC
CATGACAATGCCGTAACCATGTGCCGCCTTCTGGGCGTGGAACTTCGTGAAATCCCCATCTCGGAATGCTGCCTCCGGCA
CTTCGCCGACATCGGGCACGATCCTGCCGAACGAACCACCACTTATGAAAACGTCCAGGCCCGCGAACGCACGCAAATTC
TGATGGATTTGGCCAATAAAACCGGGGGGCTGGTCGTGGGAACAGGGGACTTGTCGGAAATTGCCCTGGGATGGAGCACC
TACAATGGGGACCACATGTCCATGTACGCGGTCAACTGCTCCATTCCGAAAACGCTGATACGCTGCCTCATTGAACACAT
CGCGGAAGAATCATCCCCGGAACTGGCAGCCACCCTCGCGGACATCAACAACACTCCCGTCAGCCCGGAACTCCTGCCGC
CGTCGGACGACGGCACCATTGAGCAGAAAACGGAAGATGTGCTTGGGCCTTACGACCTGCACGACTTTTTCCTCTTCCAC
TTCATTAAATACGGTGCGGAACCGGATAAAATCCTGCACCTGGCGGAACATGCCTTCCGCGGCGAATTTCAGCCGGACTT
CATCCGCCGCTGCCTCGGCATCTTCATCCGCCGTTTCTTCCGTCAGCAATTCAAGCGCAGCTGCATGCCGGACGGCCCCA
AGGTGGGCACCATTTCCCTTTCCCCGCGCGGGGACTGGCGCATGCCTTCCGACGCCTGCGGAACCGTGTGGGAAAAAGCC
ATCCCCCACTACTGA

Upstream 100 bases:

>100_bases
GAACTTTTAGTATCCGGCTCAGGCCCGCCTTTCCCGGATTGTGTCCTGACTACGGGAAAAACGAACATCTGCGGGTTCCA
TTTTTCAGACTGCGCCGCCC

Downstream 100 bases:

>100_bases
GACCTCCTATCCGGAAAGTTGCGGAGACGGCTTTTCCCGGAACCCGGCAAGGATGCCGAAGACCGCATCAATGCCCGGGG
GCCAACTTCAGCCCGATAAT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MFGYYRLASAVPQLRVAEVDYNVDQLTEGFRKAAEQQAAAVVFPELCITGYSCGDLFFQPNLRKAALNGLLRFTEATEGS
GTIAVVGLPFLHEDALYNTAAVVQSGRILALVPKTVLPNYREFYEKRQFTSGRELGTGVKEVTVNGVHIPFGTEIVFHEE
SSPFSFGVEICEDLWSVIPPSSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGRCLCAYVLSSAGVHESTTDTVFG
GHSLIADNGRPAAEGERFCRESTLIFADVDFERLEAARLSESSFNDSKSLFPAGNALHLALPEQVPGAPGLEYAFNPALP
FLPSPSRRRERCEEIISIQTAGLAKRMEHTRAQRLVIGISGGLDSTLALLICSRACRALKRPASDILAVTMPGFGTTDRT
HDNAVTMCRLLGVELREIPISECCLRHFADIGHDPAERTTTYENVQARERTQILMDLANKTGGLVVGTGDLSEIALGWST
YNGDHMSMYAVNCSIPKTLIRCLIEHIAEESSPELAATLADINNTPVSPELLPPSDDGTIEQKTEDVLGPYDLHDFFLFH
FIKYGAEPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFFRQQFKRSCMPDGPKVGTISLSPRGDWRMPSDACGTVWEKA
IPHY

Sequences:

>Translated_644_residues
MFGYYRLASAVPQLRVAEVDYNVDQLTEGFRKAAEQQAAAVVFPELCITGYSCGDLFFQPNLRKAALNGLLRFTEATEGS
GTIAVVGLPFLHEDALYNTAAVVQSGRILALVPKTVLPNYREFYEKRQFTSGRELGTGVKEVTVNGVHIPFGTEIVFHEE
SSPFSFGVEICEDLWSVIPPSSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGRCLCAYVLSSAGVHESTTDTVFG
GHSLIADNGRPAAEGERFCRESTLIFADVDFERLEAARLSESSFNDSKSLFPAGNALHLALPEQVPGAPGLEYAFNPALP
FLPSPSRRRERCEEIISIQTAGLAKRMEHTRAQRLVIGISGGLDSTLALLICSRACRALKRPASDILAVTMPGFGTTDRT
HDNAVTMCRLLGVELREIPISECCLRHFADIGHDPAERTTTYENVQARERTQILMDLANKTGGLVVGTGDLSEIALGWST
YNGDHMSMYAVNCSIPKTLIRCLIEHIAEESSPELAATLADINNTPVSPELLPPSDDGTIEQKTEDVLGPYDLHDFFLFH
FIKYGAEPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFFRQQFKRSCMPDGPKVGTISLSPRGDWRMPSDACGTVWEKA
IPHY
>Mature_644_residues
MFGYYRLASAVPQLRVAEVDYNVDQLTEGFRKAAEQQAAAVVFPELCITGYSCGDLFFQPNLRKAALNGLLRFTEATEGS
GTIAVVGLPFLHEDALYNTAAVVQSGRILALVPKTVLPNYREFYEKRQFTSGRELGTGVKEVTVNGVHIPFGTEIVFHEE
SSPFSFGVEICEDLWSVIPPSSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGRCLCAYVLSSAGVHESTTDTVFG
GHSLIADNGRPAAEGERFCRESTLIFADVDFERLEAARLSESSFNDSKSLFPAGNALHLALPEQVPGAPGLEYAFNPALP
FLPSPSRRRERCEEIISIQTAGLAKRMEHTRAQRLVIGISGGLDSTLALLICSRACRALKRPASDILAVTMPGFGTTDRT
HDNAVTMCRLLGVELREIPISECCLRHFADIGHDPAERTTTYENVQARERTQILMDLANKTGGLVVGTGDLSEIALGWST
YNGDHMSMYAVNCSIPKTLIRCLIEHIAEESSPELAATLADINNTPVSPELLPPSDDGTIEQKTEDVLGPYDLHDFFLFH
FIKYGAEPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFFRQQFKRSCMPDGPKVGTISLSPRGDWRMPSDACGTVWEKA
IPHY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=596, Percent_Identity=23.489932885906, Blast_Score=100, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17538756, Length=622, Percent_Identity=24.5980707395498, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=380, Percent_Identity=25, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24641841, Length=605, Percent_Identity=23.4710743801653, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641843, Length=605, Percent_Identity=23.4710743801653, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 70867; Mature: 70867

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYYRLASAVPQLRVAEVDYNVDQLTEGFRKAAEQQAAAVVFPELCITGYSCGDLFFQP
CCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCEECC
NLRKAALNGLLRFTEATEGSGTIAVVGLPFLHEDALYNTAAVVQSGRILALVPKTVLPNY
CHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCH
REFYEKRQFTSGRELGTGVKEVTVNGVHIPFGTEIVFHEESSPFSFGVEICEDLWSVIPP
HHHHHHHHCCCCHHHCCCHHHEEECCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
SSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGRCLCAYVLSSAGVHESTTDTVFG
CCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCEEC
GHSLIADNGRPAAEGERFCRESTLIFADVDFERLEAARLSESSFNDSKSLFPAGNALHLA
CCEEEECCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
LPEQVPGAPGLEYAFNPALPFLPSPSRRRERCEEIISIQTAGLAKRMEHTRAQRLVIGIS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GGLDSTLALLICSRACRALKRPASDILAVTMPGFGTTDRTHDNAVTMCRLLGVELREIPI
CCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHCCH
SECCLRHFADIGHDPAERTTTYENVQARERTQILMDLANKTGGLVVGTGDLSEIALGWST
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHEEEEEE
YNGDHMSMYAVNCSIPKTLIRCLIEHIAEESSPELAATLADINNTPVSPELLPPSDDGTI
CCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
EQKTEDVLGPYDLHDFFLFHFIKYGAEPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFF
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RQQFKRSCMPDGPKVGTISLSPRGDWRMPSDACGTVWEKAIPHY
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MFGYYRLASAVPQLRVAEVDYNVDQLTEGFRKAAEQQAAAVVFPELCITGYSCGDLFFQP
CCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCEECC
NLRKAALNGLLRFTEATEGSGTIAVVGLPFLHEDALYNTAAVVQSGRILALVPKTVLPNY
CHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCH
REFYEKRQFTSGRELGTGVKEVTVNGVHIPFGTEIVFHEESSPFSFGVEICEDLWSVIPP
HHHHHHHHCCCCHHHCCCHHHEEECCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
SSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGRCLCAYVLSSAGVHESTTDTVFG
CCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCEEC
GHSLIADNGRPAAEGERFCRESTLIFADVDFERLEAARLSESSFNDSKSLFPAGNALHLA
CCEEEECCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
LPEQVPGAPGLEYAFNPALPFLPSPSRRRERCEEIISIQTAGLAKRMEHTRAQRLVIGIS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GGLDSTLALLICSRACRALKRPASDILAVTMPGFGTTDRTHDNAVTMCRLLGVELREIPI
CCHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHCCH
SECCLRHFADIGHDPAERTTTYENVQARERTQILMDLANKTGGLVVGTGDLSEIALGWST
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHEEEEEE
YNGDHMSMYAVNCSIPKTLIRCLIEHIAEESSPELAATLADINNTPVSPELLPPSDDGTI
CCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
EQKTEDVLGPYDLHDFFLFHFIKYGAEPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFF
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RQQFKRSCMPDGPKVGTISLSPRGDWRMPSDACGTVWEKAIPHY
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]