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The map label for this gene is bioB

Identifier: 187734999

GI number: 187734999

Start: 582473

End: 583438

Strand: Direct

Name: bioB

Synonym: Amuc_0492

Alternate gene names: 187734999

Gene position: 582473-583438 (Clockwise)

Preceding gene: 187734998

Following gene: 187735000

Centisome position: 21.86

GC content: 57.35

Gene sequence:

>966_bases
ATGAGTTTGACATCATCCCTCCTTTCCAGGGTGCTCGGAGGCGGTTCCTGCTCCAGAGAAGAATTGATAGCCCTCAGCCG
GGAACCGCTGGAAGAATTGTGCCAGGCGGCCAACGCCATCCGGGAACATTTTTGCGGGAATGTCTTCGACCTCTGCACCA
TCATCAACGGCCGCAGCGGCAAATGTTCGGAAAACTGCAAATACTGCGCCCAGTCCGCCCATTACTCCACGGCAGTGGAG
GAATATCCCCTTTTAAGTGATGAAGCCCTCCTGGCGGGGGCCAGGTACAATGACGCACGTGGCATTCTGCGCTACTCCAT
CGTCACCTCCGGCAAGAGGCTGACGGATGAAGACGTGGACCGGCTCTGCGCCAGTTACCGGCACATCGCCGAACATTGCG
GCATCTCCCTCTGCGCCTCCCACGGCCTTATTTCCAAGAAACACTGCGAGCAATTGAAAGCCGCAGGCGTTTCACGCTAC
CACAATAATCTGGAAACTTCCCGCCGCAATTTCCCTAATGTCTGCACCACCCATACGTACGACGACAAACTGCAAACCAT
CAAATGGGCTCTGGAAGCCGGGCTGGAAGTTTGCAGCGGCGGCATTATGGGGCTTGGAGAGACTATGGAAGACCGGATAG
ACATGTACATGGACATCGCGGCGCTGGGCATCAAATCCATGCCCGTCAACTTTCTGACCCCCATCCCCGGAACGCCGTAC
GCGGACATGACCCCCCTGGGAGAAGAAGAACAGCTGCGTATCGTGGCCCTGGTGCGGTTCATCATGCCGGACGGCTTCGT
CCGCATCGCCGCCGGCAGGAATACCATGAAGGACCACGGCAGGAAAATCTTCATGTCCGGAGCGAATGCCGCCATCTCCG
GAGATATGCTTACCACCGCCGGCGTCACCATCCGGGAAGACCTGGCCATGCTGGCGGAACTGGGATATGAAGTCCGCATG
AAATAA

Upstream 100 bases:

>100_bases
ACAGGTAGCCGAATAAGAGAACGGGAAAGAAGGTTCCACGATCCGCCTCCTTCGCCATTCTGACCTTGACGTGAACGGAG
CCGGGGTTAGTCTTTCACGC

Downstream 100 bases:

>100_bases
GGTCCGTCCCATTTTCGCGCCCTTTGCCATGAATCACTCCGACTGGTCAAAAAAAGATCTGGAATACATCTGGCATCCCT
GTTCCCAGATGAAGGATTAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSLTSSLLSRVLGGGSCSREELIALSREPLEELCQAANAIREHFCGNVFDLCTIINGRSGKCSENCKYCAQSAHYSTAVE
EYPLLSDEALLAGARYNDARGILRYSIVTSGKRLTDEDVDRLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRY
HNNLETSRRNFPNVCTTHTYDDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMDIAALGIKSMPVNFLTPIPGTPY
ADMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTMKDHGRKIFMSGANAAISGDMLTTAGVTIREDLAMLAELGYEVRM
K

Sequences:

>Translated_321_residues
MSLTSSLLSRVLGGGSCSREELIALSREPLEELCQAANAIREHFCGNVFDLCTIINGRSGKCSENCKYCAQSAHYSTAVE
EYPLLSDEALLAGARYNDARGILRYSIVTSGKRLTDEDVDRLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRY
HNNLETSRRNFPNVCTTHTYDDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMDIAALGIKSMPVNFLTPIPGTPY
ADMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTMKDHGRKIFMSGANAAISGDMLTTAGVTIREDLAMLAELGYEVRM
K
>Mature_320_residues
SLTSSLLSRVLGGGSCSREELIALSREPLEELCQAANAIREHFCGNVFDLCTIINGRSGKCSENCKYCAQSAHYSTAVEE
YPLLSDEALLAGARYNDARGILRYSIVTSGKRLTDEDVDRLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRYH
NNLETSRRNFPNVCTTHTYDDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMDIAALGIKSMPVNFLTPIPGTPYA
DMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTMKDHGRKIFMSGANAAISGDMLTTAGVTIREDLAMLAELGYEVRMK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=300, Percent_Identity=33.6666666666667, Blast_Score=179, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=34.4827586206897, Blast_Score=179, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_AKKM8 (B2UNW0)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877111.1
- ProteinModelPortal:   B2UNW0
- SMR:   B2UNW0
- GeneID:   6275395
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0492
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK2543109
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35190; Mature: 35059

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTSSLLSRVLGGGSCSREELIALSREPLEELCQAANAIREHFCGNVFDLCTIINGRSG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
KCSENCKYCAQSAHYSTAVEEYPLLSDEALLAGARYNDARGILRYSIVTSGKRLTDEDVD
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHEEEHHHHCCCCCCHHHHH
RLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRYHNNLETSRRNFPNVCTTHTY
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMDIAALGIKSMPVNFLTPIPGTPY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
ADMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTMKDHGRKIFMSGANAAISGDMLTTA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCEEEEECCCCEECCCCHHHC
GVTIREDLAMLAELGYEVRMK
CCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLTSSLLSRVLGGGSCSREELIALSREPLEELCQAANAIREHFCGNVFDLCTIINGRSG
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
KCSENCKYCAQSAHYSTAVEEYPLLSDEALLAGARYNDARGILRYSIVTSGKRLTDEDVD
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHEEEHHHHCCCCCCHHHHH
RLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRYHNNLETSRRNFPNVCTTHTY
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMDIAALGIKSMPVNFLTPIPGTPY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
ADMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTMKDHGRKIFMSGANAAISGDMLTTA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCEEEEECCCCEECCCCHHHC
GVTIREDLAMLAELGYEVRMK
CCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA