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The map label for this gene is engD [H]

Identifier: 187734952

GI number: 187734952

Start: 528747

End: 529868

Strand: Direct

Name: engD [H]

Synonym: Amuc_0445

Alternate gene names: 187734952

Gene position: 528747-529868 (Clockwise)

Preceding gene: 187734951

Following gene: 187734953

Centisome position: 19.85

GC content: 56.86

Gene sequence:

>1122_bases
ATGTTACAGGCAGGTATTGTAGGTCTCCCCAACGTCGGCAAATCCACTCTATTCAATGCGGTCACCCGGACGCGCAAGGC
CCAGGCGGCCAACTATCCCTTCTGCACCATTGACCCGAACGTGGGTATGGTAACAGTCCCGGATCCCCGCCTGCAGGTTC
TTTCCGACATGTCCGGTTCTGAAAAAATCATCCCCACGCTTATTGAATTCGTGGATATTGCCGGTCTGGTCAAGGGAGCC
TCGGAAGGAGCCGGCCTGGGCAACCAGTTTCTGGCGAACATCCGTGAAGTGGACGCCATCGTGCAGGTAGTCCGCTGCTT
TGACAATGATGATATTATCCATGAACTCGGTTCCGTGGATCCTCTGCGCGACATTGACATCATCAACTCGGAACTCATTC
TGGCGGATATCGCCACCATGGAAAAGAGACTCTCTTCCCGCGAACGCAAGGCACGCAGCGGCGACAAGGAAGCCAAGGCC
GAAGTGGCCCTCATCACCAAACTCCTGCCCCACCTGAACGAAGGCAATCCCGCCCTCACCCTTGAACCGCAGCTGGACGA
CGACGAACGCAAGCTGCTCCATTCTTTCCAGCTGCTTTCGGATAAAAAAAGCATTTTCGCCTGCAATGTCAATGAAGACG
AACTGGCGGACGCCATCTCCAACCCGGACGCCCATCCCTACGTCTCCCAGGTGAAAAAATACGTAGCGGAACACCATAAC
GCGGAAGCCATTGTCATCTCCGCCCGGATTGAAGAGGAACTCATCGACGTCTCGGAGGAAGAATCCCGCGAATTCCTGGA
ATCCCTGGGTGTGAAGGACTCCGGCGTTTCCGACCTTATCCGCGCCGTGTACCACCTTCTGGGCCTGCGCACCTACCTCA
CCACGGGAATCAAGGAAACGCGGGCCTGGACCATTCCGGCCGGGGCAAAAGCACCCCAGGCGGCCGGCGTCATCCACACG
GACTTTGAACGCGGTTTTATCGCCGCGGAGGTAGTGCACTACGACGACCTGGTTTCCTGCGGCGGCAAGGCCGGAGCGAG
GGAACACGGCAAGCTGCGCATCGAAGGAAAAGAATATGTCGTCAAAGACGGGGACGTCATCGAATTCCGCTTCAATGTCT
AG

Upstream 100 bases:

>100_bases
TCGGAAAACCTGATCAGATGGGCTTTGGGATCCGCCGCATAAATGCCCGTCGGCAATTGGTATTTGACTTTCACGGCGTG
ACCCGCTATCAGTCCTGCCT

Downstream 100 bases:

>100_bases
ACGGAAGCGTTACCGGAATTCCATTGACTTTTACTGATATAAGGGGCAGATTGCTCATAACCTTCACATCGCCTCATGAC
TGATACAGACGTCAAATCCA

Product: GTP-binding protein YchF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLQAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNVGMVTVPDPRLQVLSDMSGSEKIIPTLIEFVDIAGLVKGA
SEGAGLGNQFLANIREVDAIVQVVRCFDNDDIIHELGSVDPLRDIDIINSELILADIATMEKRLSSRERKARSGDKEAKA
EVALITKLLPHLNEGNPALTLEPQLDDDERKLLHSFQLLSDKKSIFACNVNEDELADAISNPDAHPYVSQVKKYVAEHHN
AEAIVISARIEEELIDVSEEESREFLESLGVKDSGVSDLIRAVYHLLGLRTYLTTGIKETRAWTIPAGAKAPQAAGVIHT
DFERGFIAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYVVKDGDVIEFRFNV

Sequences:

>Translated_373_residues
MLQAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNVGMVTVPDPRLQVLSDMSGSEKIIPTLIEFVDIAGLVKGA
SEGAGLGNQFLANIREVDAIVQVVRCFDNDDIIHELGSVDPLRDIDIINSELILADIATMEKRLSSRERKARSGDKEAKA
EVALITKLLPHLNEGNPALTLEPQLDDDERKLLHSFQLLSDKKSIFACNVNEDELADAISNPDAHPYVSQVKKYVAEHHN
AEAIVISARIEEELIDVSEEESREFLESLGVKDSGVSDLIRAVYHLLGLRTYLTTGIKETRAWTIPAGAKAPQAAGVIHT
DFERGFIAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYVVKDGDVIEFRFNV
>Mature_373_residues
MLQAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNVGMVTVPDPRLQVLSDMSGSEKIIPTLIEFVDIAGLVKGA
SEGAGLGNQFLANIREVDAIVQVVRCFDNDDIIHELGSVDPLRDIDIINSELILADIATMEKRLSSRERKARSGDKEAKA
EVALITKLLPHLNEGNPALTLEPQLDDDERKLLHSFQLLSDKKSIFACNVNEDELADAISNPDAHPYVSQVKKYVAEHHN
AEAIVISARIEEELIDVSEEESREFLESLGVKDSGVSDLIRAVYHLLGLRTYLTTGIKETRAWTIPAGAKAPQAAGVIHT
DFERGFIAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYVVKDGDVIEFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=374, Percent_Identity=41.1764705882353, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI58761502, Length=224, Percent_Identity=34.8214285714286, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI24308117, Length=199, Percent_Identity=29.1457286432161, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787454, Length=372, Percent_Identity=49.7311827956989, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789574, Length=142, Percent_Identity=32.3943661971831, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17509631, Length=374, Percent_Identity=39.8395721925134, Blast_Score=266, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6319499, Length=378, Percent_Identity=42.0634920634921, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6321773, Length=384, Percent_Identity=35.15625, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321649, Length=296, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640873, Length=373, Percent_Identity=39.4101876675603, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640877, Length=373, Percent_Identity=39.4101876675603, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640875, Length=373, Percent_Identity=39.4101876675603, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640879, Length=331, Percent_Identity=36.8580060422961, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24585318, Length=142, Percent_Identity=33.8028169014084, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40818; Mature: 40818

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNVGMVTVPDPRLQVLSDMSGS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHCCCCC
EKIIPTLIEFVDIAGLVKGASEGAGLGNQFLANIREVDAIVQVVRCFDNDDIIHELGSVD
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
PLRDIDIINSELILADIATMEKRLSSRERKARSGDKEAKAEVALITKLLPHLNEGNPALT
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LEPQLDDDERKLLHSFQLLSDKKSIFACNVNEDELADAISNPDAHPYVSQVKKYVAEHHN
ECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
AEAIVISARIEEELIDVSEEESREFLESLGVKDSGVSDLIRAVYHLLGLRTYLTTGIKET
CCEEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHC
RAWTIPAGAKAPQAAGVIHTDFERGFIAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYV
CEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEE
VKDGDVIEFRFNV
EECCCEEEEEECC
>Mature Secondary Structure
MLQAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNVGMVTVPDPRLQVLSDMSGS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHHHHHHHCCCCC
EKIIPTLIEFVDIAGLVKGASEGAGLGNQFLANIREVDAIVQVVRCFDNDDIIHELGSVD
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
PLRDIDIINSELILADIATMEKRLSSRERKARSGDKEAKAEVALITKLLPHLNEGNPALT
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LEPQLDDDERKLLHSFQLLSDKKSIFACNVNEDELADAISNPDAHPYVSQVKKYVAEHHN
ECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
AEAIVISARIEEELIDVSEEESREFLESLGVKDSGVSDLIRAVYHLLGLRTYLTTGIKET
CCEEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHC
RAWTIPAGAKAPQAAGVIHTDFERGFIAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYV
CEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEE
VKDGDVIEFRFNV
EECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]