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The map label for this gene is dacB [H]

Identifier: 187734951

GI number: 187734951

Start: 527765

End: 528688

Strand: Direct

Name: dacB [H]

Synonym: Amuc_0444

Alternate gene names: 187734951

Gene position: 527765-528688 (Clockwise)

Preceding gene: 187734950

Following gene: 187734952

Centisome position: 19.81

GC content: 59.74

Gene sequence:

>924_bases
ATGCGTTTATTCGCCCTTCTTGCAGGATTCTCCCTTTGCCTGAGCTCCACAAGCTGCCAATCGTACGGGGATTCAAGTGA
CTACATCCCCCTTGCAACGCCCGTTCCCCCACAACTCTCCCAGCTGGTTTCCCATGCCCTCCCCCGACCGGCCAATTTCC
CCACACGTCCCGTAGCCATCCCCTCAAGCGCCCCGCGCACGCCCCGCTGCGCCAGCGCCTGCGTCATGGACGCCCTTACC
GGCAAAGTGCTCTTTTCCCACAACGGCCTCCAGCACCGCCAGGTAGCCAGCACGCAGAAACTCGTGACGGCCATGGTCGT
GATGGAACACGGCAGCCTGGACAAAAAAGTGGTCATCCAGCCTTCCGATACCAAGGCGGACCCCACCAAACTGGGATTCC
GGGCGGGGGAAGTCTATTCACGCCGCGAACTGCTCAATGCCATGATGATCCGCAGTTTCAACGACGTAGCCCTGGCTCTG
GCCCGCGATACGGCGGGTTCCGTTCCCCGTTTCGCCCAGTTGATGAATGCCAAAGCCCGGCAGATGGGCATGTACAACTC
ACGCTTCGCCAATCCCAACGGCCTCCCGGCGGATCAATATTCCACTGCCATTGACATGGCCCGCTGCGCCTATTATGTGT
ACCGCAACCCGGAACTGCGCAATATTATCTGCAAACGCCAATATGCCTTTACCCGCGCCAACGGCAGAACGCTTCTGCTG
AGAAACACCAACAAGCTGCTCACCCAGAACCCCTGGGTTACCGGAATGAAAACCGGATACACGAACGCCGCCGGACGCTG
CCTGGTTTCCTCAGCCGGCGTCAACGGGCGCCATGTCATCGTCGTCGTCCTCGGCTGCCATCCGTCCCGTATCTGGACGG
AATCGGAAAACCTGATCAGATGGGCTTTGGGATCCGCCGCATAA

Upstream 100 bases:

>100_bases
AGGCCGCGGATTGTCCGATCCGCGGCCTTTTTCGTCCGGCTCTTTTTCAAATGCGGAGTTTGGGCTTGATGAACGAAACT
TCTTACTGTAGCAACAAGAC

Downstream 100 bases:

>100_bases
ATGCCCGTCGGCAATTGGTATTTGACTTTCACGGCGTGACCCGCTATCAGTCCTGCCTATGTTACAGGCAGGTATTGTAG
GTCTCCCCAACGTCGGCAAA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRLFALLAGFSLCLSSTSCQSYGDSSDYIPLATPVPPQLSQLVSHALPRPANFPTRPVAIPSSAPRTPRCASACVMDALT
GKVLFSHNGLQHRQVASTQKLVTAMVVMEHGSLDKKVVIQPSDTKADPTKLGFRAGEVYSRRELLNAMMIRSFNDVALAL
ARDTAGSVPRFAQLMNAKARQMGMYNSRFANPNGLPADQYSTAIDMARCAYYVYRNPELRNIICKRQYAFTRANGRTLLL
RNTNKLLTQNPWVTGMKTGYTNAAGRCLVSSAGVNGRHVIVVVLGCHPSRIWTESENLIRWALGSAA

Sequences:

>Translated_307_residues
MRLFALLAGFSLCLSSTSCQSYGDSSDYIPLATPVPPQLSQLVSHALPRPANFPTRPVAIPSSAPRTPRCASACVMDALT
GKVLFSHNGLQHRQVASTQKLVTAMVVMEHGSLDKKVVIQPSDTKADPTKLGFRAGEVYSRRELLNAMMIRSFNDVALAL
ARDTAGSVPRFAQLMNAKARQMGMYNSRFANPNGLPADQYSTAIDMARCAYYVYRNPELRNIICKRQYAFTRANGRTLLL
RNTNKLLTQNPWVTGMKTGYTNAAGRCLVSSAGVNGRHVIVVVLGCHPSRIWTESENLIRWALGSAA
>Mature_307_residues
MRLFALLAGFSLCLSSTSCQSYGDSSDYIPLATPVPPQLSQLVSHALPRPANFPTRPVAIPSSAPRTPRCASACVMDALT
GKVLFSHNGLQHRQVASTQKLVTAMVVMEHGSLDKKVVIQPSDTKADPTKLGFRAGEVYSRRELLNAMMIRSFNDVALAL
ARDTAGSVPRFAQLMNAKARQMGMYNSRFANPNGLPADQYSTAIDMARCAYYVYRNPELRNIICKRQYAFTRANGRTLLL
RNTNKLLTQNPWVTGMKTGYTNAAGRCLVSSAGVNGRHVIVVVLGCHPSRIWTESENLIRWALGSAA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=35.2678571428571, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787062, Length=245, Percent_Identity=33.469387755102, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082022, Length=265, Percent_Identity=31.6981132075472, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786851, Length=261, Percent_Identity=31.4176245210728, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 33579; Mature: 33579

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFALLAGFSLCLSSTSCQSYGDSSDYIPLATPVPPQLSQLVSHALPRPANFPTRPVAI
CHHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEC
PSSAPRTPRCASACVMDALTGKVLFSHNGLQHRQVASTQKLVTAMVVMEHGSLDKKVVIQ
CCCCCCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
PSDTKADPTKLGFRAGEVYSRRELLNAMMIRSFNDVALALARDTAGSVPRFAQLMNAKAR
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHH
QMGMYNSRFANPNGLPADQYSTAIDMARCAYYVYRNPELRNIICKRQYAFTRANGRTLLL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHEEEECCCCEEEE
RNTNKLLTQNPWVTGMKTGYTNAAGRCLVSSAGVNGRHVIVVVLGCHPSRIWTESENLIR
ECCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEECCCCCCEE
WALGSAA
ECCCCCC
>Mature Secondary Structure
MRLFALLAGFSLCLSSTSCQSYGDSSDYIPLATPVPPQLSQLVSHALPRPANFPTRPVAI
CHHHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEC
PSSAPRTPRCASACVMDALTGKVLFSHNGLQHRQVASTQKLVTAMVVMEHGSLDKKVVIQ
CCCCCCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
PSDTKADPTKLGFRAGEVYSRRELLNAMMIRSFNDVALALARDTAGSVPRFAQLMNAKAR
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHH
QMGMYNSRFANPNGLPADQYSTAIDMARCAYYVYRNPELRNIICKRQYAFTRANGRTLLL
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHEEEECCCCEEEE
RNTNKLLTQNPWVTGMKTGYTNAAGRCLVSSAGVNGRHVIVVVLGCHPSRIWTESENLIR
ECCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCEECCCCCCEE
WALGSAA
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]