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The map label for this gene is pyk [H]

Identifier: 187734938

GI number: 187734938

Start: 515311

End: 516732

Strand: Direct

Name: pyk [H]

Synonym: Amuc_0431

Alternate gene names: 187734938

Gene position: 515311-516732 (Clockwise)

Preceding gene: 187734936

Following gene: 187734939

Centisome position: 19.34

GC content: 59.85

Gene sequence:

>1422_bases
ATGAACACACCTTCCCGGCCCCGGGCCACCAAAATCATCTGCACTATTGGACCAGCAACAGACACTTCCGACATGCTGGG
ACAGCTTATTGAAGCCGGAGCCAACGTCTTCCGGCTCAATATGAGCCATTCCAAACACGACTGGGTCCGGGAAGTCGTCT
TCCGCATCCGCCGGAAAGCAGCGGAGCTGGAAGCGAACGTGGCCATCCTATTTGACCTCCAGGGTCCCTCCATCCGTACG
GGAGACCTGGCCGAACCCTACCATTTGAAAAAGGGAGACACGGTAGAAATCCGCCTGGGCACGGCCAAGCCGGAACTTCC
TTTTTCCACCACGGTAAACTATGACGGCCTTCTGCAGGATGTTCAGGCGGGCCATACCATGGTGGTGGATAACGGCGGCA
TCCTCATGCGCATTGCGGAAGTACGCCCGGACAGGCTCATCTGCGACGTGCTCACGGAAGGCGTATTCGGCTCCCGCCGC
CACATCAACCTTCCCGGCGTAGCGCTGCGGCTCCCGGCCCTGACGGAAAAGGATCTGGCGGACCTGGCCGTGGCCGTAGA
ATGCGAAACGGACTACGTAGCCATGTCCTTCGTCCGGGATGCCGCCCACATTGCCCAGCTCCGGGAACACATCGACAACT
TGGGAGGCAAGGCCCAGATCGTCGCAAAAATTGAAGACCAGCAGGCTGTCCGACACATTGACGATATCATCCTGGCCGCA
GACGTCATCATGGTCGCGCGCGGGGACCTGGGCATTGAAGTCAGCATTGAAGAACTGCCCATCATCCAGCGCCGCATCGT
CCGCCATTGCCACAGGCTGGGACGCCGCTGCATCGTAGCAACGCATATGCTGGAATCCATGATAACCCAGCCCACCCCTA
CCCGTGCGGAAGTCACGGATGTTTCCAACGCCATCTTTGAACAAGTGGATGCCGTCATGCTTTCCGGAGAAACCTCCGTG
GGCCATTACCCCGTACGCTGTGTGGAAGTGCTGGATTCCATCGCCCACCGCATGGAGTGCTCCGGCAATCTCAACTTCGC
GGCATCCGCCATCCTGAACGGAGACCGCCAGAAAGCCACCAAGGCAGCCATCTCCCTGGCGGACTCCGTGGAAAACGCCT
GTCTGATCGTCTTTACCCGCCGCGGCCTGGCCGCCACGCAGGCAGCCGTCCTCAGGCCGGAACGGGCTGCCATCTTCGCC
TTCAGCAATGACCCTGTCGTTGTCAGGCAACTCGCCCTGGCGCGTGACGTAACCGCCTTTGAATCTCCCTTCCTGAAAGA
CCCGGCCCGCATGATTTCCACCGCCCTGGACCTGCTGAAGGAAAAAGGGCTGATCTCCTCCGGCCAGCCTGTCGTCATCC
AGGGGGACAGCCTGCAAGGGGAACTGCTGGCGGATTCCATTATCTTCATGCGCGTGGAATAA

Upstream 100 bases:

>100_bases
CTCCCTCAAGCACCCCGTGACATGCAACAATCCTGCCATGTATCATAAAAAACTTCATTTAAACTCCCCGTAAGATACAT
TAGGGGCACTGACAAAGACC

Downstream 100 bases:

>100_bases
TGGCCTGCACCTTTCCTATAGAGCGGGAGACGGCCGTGTGGCCCCCGTCCGCCCCCCTCAGGGAACTCCTGCTGGATGCG
GATCCGGACGAACGCCGGAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHSKHDWVREVVFRIRRKAAELEANVAILFDLQGPSIRT
GDLAEPYHLKKGDTVEIRLGTAKPELPFSTTVNYDGLLQDVQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRR
HINLPGVALRLPALTEKDLADLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKAQIVAKIEDQQAVRHIDDIILAA
DVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRRCIVATHMLESMITQPTPTRAEVTDVSNAIFEQVDAVMLSGETSV
GHYPVRCVEVLDSIAHRMECSGNLNFAASAILNGDRQKATKAAISLADSVENACLIVFTRRGLAATQAAVLRPERAAIFA
FSNDPVVVRQLALARDVTAFESPFLKDPARMISTALDLLKEKGLISSGQPVVIQGDSLQGELLADSIIFMRVE

Sequences:

>Translated_473_residues
MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHSKHDWVREVVFRIRRKAAELEANVAILFDLQGPSIRT
GDLAEPYHLKKGDTVEIRLGTAKPELPFSTTVNYDGLLQDVQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRR
HINLPGVALRLPALTEKDLADLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKAQIVAKIEDQQAVRHIDDIILAA
DVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRRCIVATHMLESMITQPTPTRAEVTDVSNAIFEQVDAVMLSGETSV
GHYPVRCVEVLDSIAHRMECSGNLNFAASAILNGDRQKATKAAISLADSVENACLIVFTRRGLAATQAAVLRPERAAIFA
FSNDPVVVRQLALARDVTAFESPFLKDPARMISTALDLLKEKGLISSGQPVVIQGDSLQGELLADSIIFMRVE
>Mature_473_residues
MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHSKHDWVREVVFRIRRKAAELEANVAILFDLQGPSIRT
GDLAEPYHLKKGDTVEIRLGTAKPELPFSTTVNYDGLLQDVQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRR
HINLPGVALRLPALTEKDLADLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKAQIVAKIEDQQAVRHIDDIILAA
DVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRRCIVATHMLESMITQPTPTRAEVTDVSNAIFEQVDAVMLSGETSV
GHYPVRCVEVLDSIAHRMECSGNLNFAASAILNGDRQKATKAAISLADSVENACLIVFTRRGLAATQAAVLRPERAAIFA
FSNDPVVVRQLALARDVTAFESPFLKDPARMISTALDLLKEKGLISSGQPVVIQGDSLQGELLADSIIFMRVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=478, Percent_Identity=35.7740585774059, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI33286422, Length=478, Percent_Identity=35.5648535564854, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI33286420, Length=478, Percent_Identity=35.5648535564854, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI10835121, Length=476, Percent_Identity=34.453781512605, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI32967597, Length=476, Percent_Identity=34.453781512605, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.7697594501718, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.7697594501718, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=35, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=35, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787965, Length=430, Percent_Identity=36.046511627907, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=37.3165618448637, Blast_Score=251, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=34.0909090909091, Blast_Score=262, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=35.5371900826446, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=35.5371900826446, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=35.5371900826446, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=35.5371900826446, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=338, Percent_Identity=37.5739644970414, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=29.9180327868852, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=35.8452138492872, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=35.8452138492872, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24648966, Length=320, Percent_Identity=41.25, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24581235, Length=473, Percent_Identity=30.8668076109937, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24646914, Length=180, Percent_Identity=42.7777777777778, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51690; Mature: 51690

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHSKHDWVREVVFRIRRKA
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AELEANVAILFDLQGPSIRTGDLAEPYHLKKGDTVEIRLGTAKPELPFSTTVNYDGLLQD
HHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEECCHHHHHHH
VQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRRHINLPGVALRLPALTEKDLA
HHCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCCCEECCCCCEEECCCCCCCCHH
DLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKAQIVAKIEDQQAVRHIDDIILAA
HEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
DVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRRCIVATHMLESMITQPTPTRAEVTD
HHHEEECCCCCEEEEHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHH
VSNAIFEQVDAVMLSGETSVGHYPVRCVEVLDSIAHRMECSGNLNFAASAILNGDRQKAT
HHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHH
KAAISLADSVENACLIVFTRRGLAATQAAVLRPERAAIFAFSNDPVVVRQLALARDVTAF
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
ESPFLKDPARMISTALDLLKEKGLISSGQPVVIQGDSLQGELLADSIIFMRVE
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHEECEEEEEEEC
>Mature Secondary Structure
MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHSKHDWVREVVFRIRRKA
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AELEANVAILFDLQGPSIRTGDLAEPYHLKKGDTVEIRLGTAKPELPFSTTVNYDGLLQD
HHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEECCHHHHHHH
VQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRRHINLPGVALRLPALTEKDLA
HHCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCCCEECCCCCEEECCCCCCCCHH
DLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKAQIVAKIEDQQAVRHIDDIILAA
HEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHH
DVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRRCIVATHMLESMITQPTPTRAEVTD
HHHEEECCCCCEEEEHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHH
VSNAIFEQVDAVMLSGETSVGHYPVRCVEVLDSIAHRMECSGNLNFAASAILNGDRQKAT
HHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHH
KAAISLADSVENACLIVFTRRGLAATQAAVLRPERAAIFAFSNDPVVVRQLALARDVTAF
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
ESPFLKDPARMISTALDLLKEKGLISSGQPVVIQGDSLQGELLADSIIFMRVE
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHEECEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]