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The map label for this gene is hemL

Identifier: 187734924

GI number: 187734924

Start: 497190

End: 498470

Strand: Direct

Name: hemL

Synonym: Amuc_0417

Alternate gene names: 187734924

Gene position: 497190-498470 (Clockwise)

Preceding gene: 187734923

Following gene: 187734925

Centisome position: 18.66

GC content: 58.31

Gene sequence:

>1281_bases
ATGAATCAGTCAGCCCAGCTCTTCTCCCGCGCGCGCTCCGTTATCCCCGGCGGAGTCAACTCCCCCGTCAGGGCCTTCCG
CAATGTGGACGGGGACCCCTTCTTTGTTCAATCCGCCAAAGGAGCCTACATCACGGATGCGGACGGGCGCCAGCTTATTG
ACTACATAGGCACATGGGGCCCGGCCATTCTGGGCCACGCTCCGCAACCCGTTCTGGACGCAGTACATGCCGCCGTGGAC
AGGGGGCTGGGCTATGGAATTCCTGCGCCGGCGGAAGTGGATATGGCGGAAATGATCACGGACATGGTTCCCTCCGTGGA
AAAAGTGCGCATGGTCAACTCCGGAACGGAAGCAACCATGTCCGCCATCAGGCTGGCTCGAGGCTATACCGGACGCCGTA
AAATCATTAAATTCATCGGCTGCTACCACGGCCATGTAGACTCCCTTCTGGTGGCGGCGGGTTCCGGAGCGCTCACCTTC
GGAGAACCGGACAGCGCGGGCGTGCCCAGGGAAATGACGCAGCTCACCCTCACCGTGCCCTACAATGACCGGGAAGCAGT
AAAAAAAGCCTTTGAACTCCATGGAAGCGACATTGCCGCCGTCATCCTGGAACCCTTTCCTGCCAATGCCGGGCTCTACT
TTCCGCAAAACGACTTCCTTCACTTCCTGCGGGAAATAACGCTCCGCCACGACACCCTTCTCATCTTTGATGAAGTCATG
ACGGGCTTCCGGGTCGCTCCCGGAGGCGTGCAGCAGCTTTACGGCATCACTCCAGACCTTACCTGCATGGGCAAAGTCAT
CGGCGGAGGCCTCCCCGTGGGAGCTTTTGGCGGCCGTTCGGAAATCATGGACTGCCTTTCTCCGCTTGGCCCCGTGTATC
AGGCGGGAACCCTGTCCGGCAATCCCGTTGCCATGGCGGCGGGATTGGCCCAGCTCCGGGAACTCCTGAAAGGGAACGCC
TATGAACGCCTGGAACAGCTCGGAGCACGCATGGAAGAAGGCATCCGCGAAGCCCTCAAAAAACACGGCAGAAATTACAC
CTTCCACCGTGCGGGCTCCATGTTCTGCCTCTTCTTCACGGAAGAGGAAGTCTATAATCTGGAATCCGCGCAAAAAGCTT
CCAAAAAACTCTTCAAATCCTTCTTCTGGAACATGCTGGAACAAGGCGTCTACTTCGCGCCTTCCCCGTACGAAACGGGC
TTTATCTCTACAGCCCATACGGAAGAAGACATTGACCGCACGGTGGAAGCTGTCCGCATCAGTCTCTCCAGGCTGGGTTG
A

Upstream 100 bases:

>100_bases
CGCTCCTGAAGCTTAAAACAGTATTGGTTCGGGAATAAGAACCCAATTTCCATGAGGGCAACCCTTTCTCAAGGATTGCC
CTTTTTGCTTCCCTCTGCAC

Downstream 100 bases:

>100_bases
CATGCCCCTGATTTGAAGTGACAGCTCACGGCCCTTCCCCTATAAGGACTTCATGAGACAAATGACAGGAACCATGCTGC
TTCTGTCCTCAGCAGTCGTT

Product: glutamate-1-semialdehyde-2,1-aminomutase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYITDADGRQLIDYIGTWGPAILGHAPQPVLDAVHAAVD
RGLGYGIPAPAEVDMAEMITDMVPSVEKVRMVNSGTEATMSAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTF
GEPDSAGVPREMTQLTLTVPYNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQNDFLHFLREITLRHDTLLIFDEVM
TGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAFGGRSEIMDCLSPLGPVYQAGTLSGNPVAMAAGLAQLRELLKGNA
YERLEQLGARMEEGIREALKKHGRNYTFHRAGSMFCLFFTEEEVYNLESAQKASKKLFKSFFWNMLEQGVYFAPSPYETG
FISTAHTEEDIDRTVEAVRISLSRLG

Sequences:

>Translated_426_residues
MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYITDADGRQLIDYIGTWGPAILGHAPQPVLDAVHAAVD
RGLGYGIPAPAEVDMAEMITDMVPSVEKVRMVNSGTEATMSAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTF
GEPDSAGVPREMTQLTLTVPYNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQNDFLHFLREITLRHDTLLIFDEVM
TGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAFGGRSEIMDCLSPLGPVYQAGTLSGNPVAMAAGLAQLRELLKGNA
YERLEQLGARMEEGIREALKKHGRNYTFHRAGSMFCLFFTEEEVYNLESAQKASKKLFKSFFWNMLEQGVYFAPSPYETG
FISTAHTEEDIDRTVEAVRISLSRLG
>Mature_426_residues
MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYITDADGRQLIDYIGTWGPAILGHAPQPVLDAVHAAVD
RGLGYGIPAPAEVDMAEMITDMVPSVEKVRMVNSGTEATMSAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTF
GEPDSAGVPREMTQLTLTVPYNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQNDFLHFLREITLRHDTLLIFDEVM
TGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAFGGRSEIMDCLSPLGPVYQAGTLSGNPVAMAAGLAQLRELLKGNA
YERLEQLGARMEEGIREALKKHGRNYTFHRAGSMFCLFFTEEEVYNLESAQKASKKLFKSFFWNMLEQGVYFAPSPYETG
FISTAHTEEDIDRTVEAVRISLSRLG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=309, Percent_Identity=28.4789644012945, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI24119277, Length=333, Percent_Identity=28.2282282282282, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI37574042, Length=338, Percent_Identity=29.8816568047337, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI226442705, Length=332, Percent_Identity=29.2168674698795, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI284507298, Length=228, Percent_Identity=30.2631578947368, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI226442709, Length=271, Percent_Identity=30.9963099630996, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=53.7558685446009, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=26.9230769230769, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI145693181, Length=341, Percent_Identity=29.3255131964809, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787560, Length=343, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788044, Length=283, Percent_Identity=31.4487632508834, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789759, Length=329, Percent_Identity=29.1793313069909, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786991, Length=393, Percent_Identity=26.2086513994911, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992977, Length=338, Percent_Identity=29.2899408284024, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25144271, Length=334, Percent_Identity=24.251497005988, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=316, Percent_Identity=26.5822784810127, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357415, Length=307, Percent_Identity=26.0586319218241, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_AKKM8 (B2UNE3)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877036.1
- ProteinModelPortal:   B2UNE3
- SMR:   B2UNE3
- GeneID:   6274835
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0417
- HOGENOM:   HBG725944
- OMA:   TANQFES
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46336; Mature: 46336

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYITDADGRQLIDYIGTWG
CCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHHCCC
PAILGHAPQPVLDAVHAAVDRGLGYGIPAPAEVDMAEMITDMVPSVEKVRMVNSGTEATM
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHH
SAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTFGEPDSAGVPREMTQLTLTVP
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCHHEEEEEEEC
YNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQNDFLHFLREITLRHDTLLIFDEVM
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
TGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAFGGRSEIMDCLSPLGPVYQAGTLSG
HCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCC
NPVAMAAGLAQLRELLKGNAYERLEQLGARMEEGIREALKKHGRNYTFHRAGSMFCLFFT
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
EEEVYNLESAQKASKKLFKSFFWNMLEQGVYFAPSPYETGFISTAHTEEDIDRTVEAVRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCHHHHHHHHHHHHH
SLSRLG
HHHHCC
>Mature Secondary Structure
MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYITDADGRQLIDYIGTWG
CCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECCCCCEEECCCHHHHHHHHHCCC
PAILGHAPQPVLDAVHAAVDRGLGYGIPAPAEVDMAEMITDMVPSVEKVRMVNSGTEATM
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHH
SAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTFGEPDSAGVPREMTQLTLTVP
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCHHEEEEEEEC
YNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQNDFLHFLREITLRHDTLLIFDEVM
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
TGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAFGGRSEIMDCLSPLGPVYQAGTLSG
HCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCC
NPVAMAAGLAQLRELLKGNAYERLEQLGARMEEGIREALKKHGRNYTFHRAGSMFCLFFT
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
EEEVYNLESAQKASKKLFKSFFWNMLEQGVYFAPSPYETGFISTAHTEEDIDRTVEAVRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCHHHHHHHHHHHHH
SLSRLG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA