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The map label for this gene is hldE [H]

Identifier: 187734922

GI number: 187734922

Start: 495135

End: 496625

Strand: Reverse

Name: hldE [H]

Synonym: Amuc_0415

Alternate gene names: 187734922

Gene position: 496625-495135 (Counterclockwise)

Preceding gene: 187734926

Following gene: 187734921

Centisome position: 18.64

GC content: 58.15

Gene sequence:

>1491_bases
ATGAACCGGCTGCATACATTGATTGACGGATTTTCCCGGGTGCGGATTCTCTGCATCGGGGACGTGATGCTGGATAAGTT
CCTGTATGGGAGCGTCAGCCGTATTTCGCCGGAGGCTCCGGTTCCCATCATGAAGATGGACCGGGAGACGCGCATGCTGG
GAGGCGCGGGCAATGTCGTGTCCAATCTGTGCGCGCTGGGATGCCGCACCACTTTTATCAGCGTGGTCGGGGATGATGGC
CATGGGCGGCAGGTAAGGCGTTTTCTGGAAGAGACGCACTGCGTTCCGGAGCTGGTGGAGTGCGAGGGGTATGAGACGAC
CGTGAAGATCCGTTTTGTGGCGGGCAAGCACCATTTGCTGAGAGCGGATCAGGAACAGCCGCTGAGGATGGAGCCGGAGC
TGGCTTCCCGTTTTTTGGAACGAGTGGATGCCTGTTTGCCGGAGGCGGATCTTGTCCTTTTGTCCGATTACGGGAAAGGC
CTGTTTGAGGGACGAACCGCCCCGGATGTGATTGCCCGGTGCCGTGCGGCGCGCAAGCCCGTTATTGTGGACCCGAAGGG
AGCGGATTATTCCCGCTATAGGGGGGCCACGCTGGTGAAACCCAATATGAAGGAGTTCCAGGAGGCTACCGGCGTGGCGC
TCAATCCTGCCATGGACGGGTGGGAACAGGATGCCGTGGCGGGGGCGCAGAGGCTGTTTTCCGAATTCGGCATTGAGAAT
CTGATTGTCACTTTGAGCGAACACGGCATGATTTTCATTCCTTCCTCCAGCCCTTCCGATTTTGTCTGCATTCCTACGGA
GGCCCGGGATGTTTTTGACGTGTCCGGTGCGGGTGATACCTCCCTCGCCAGTCTGGGCGCGGCGCTGGCCGCCGGAGCCG
CAGTGCCGGATGCTCTGGTCGTGTCCAATGTGGCCGCCGGCATCGTAGTGGGCAAGTTCGGAACGGCCAGCGTGACCGGA
ACGGAATTGAAGGAGGCGTTGAAGGAGAAGGATATCCAGACGCCCTCCTGGCGTCACCGCGACAATATCCTGACGCCGGA
GGCCGCGGCGGAGCTGGCGGAACGTTTCCGCCGTGAGAAGAAGGTGGTTGGATTTACAAACGGTTGTTTTGATTTGCTCC
ACCTGGGGCATCTGCATTCCTTTATGAAGGCGCGTGAAGCGTGTGACGTGCTTTTCGTAGGGTTGAATACGGACGCCTCC
ATCAAGCGGCTGAAGGGCGAGGACAGGCCGATTAATAATGAGGAGATGCGTTCCCTGCTGCTGGCATCCCTGGATTTTGT
CGATTATGTAGTGCCGTTTGATGAAGATACGGCTCTGCCGCTGATTGAGAAGCTGCGTCCGGACGTGATTGCCAAAGAAG
GGTATCCCCTGGAATTCTGGCCGGAAGGACAGTTCGTGAAATCTTATGGAGGGCAGGCTCTTGAACTGCCCCGCCTGGAG
GGCTTTTCCTCCACCAATATCATCAACCGCATGAAAAGCAGCCCGGAATGA

Upstream 100 bases:

>100_bases
ACGGCGGGAAGCGGCCGCGTTGCCCCGTTTTCCGGTTCCGGAGCGCGGTTCAACTGTCCTTGCCTTATAACAGGGGGCGT
GGTAGAAAGAGACGTCTCCC

Downstream 100 bases:

>100_bases
GCGAATACATCAGGAACCAGATTTTAGGGATAGCGGACAATTTCAAAGCCCTGGCTTCCATGGCCGGGGATATTGAGCAG
GTTGCCCGCATTTGTACGGA

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVVSNLCALGCRTTFISVVGDDG
HGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLLRADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKG
LFEGRTAPDVIARCRAARKPVIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIEN
LIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALVVSNVAAGIVVGKFGTASVTG
TELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREKKVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDAS
IKRLKGEDRPINNEEMRSLLLASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLE
GFSSTNIINRMKSSPE

Sequences:

>Translated_496_residues
MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVVSNLCALGCRTTFISVVGDDG
HGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLLRADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKG
LFEGRTAPDVIARCRAARKPVIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIEN
LIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALVVSNVAAGIVVGKFGTASVTG
TELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREKKVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDAS
IKRLKGEDRPINNEEMRSLLLASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLE
GFSSTNIINRMKSSPE
>Mature_496_residues
MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVVSNLCALGCRTTFISVVGDDG
HGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLLRADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKG
LFEGRTAPDVIARCRAARKPVIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIEN
LIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALVVSNVAAGIVVGKFGTASVTG
TELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREKKVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDAS
IKRLKGEDRPINNEEMRSLLLASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLE
GFSSTNIINRMKSSPE

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=486, Percent_Identity=37.2427983539095, Blast_Score=304, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 54442; Mature: 54442

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVV
CCHHHHHHCCHHHEEEEEEHHHHHHHHHHCCHHHCCCCCCCCHHHCCCHHHHCCCCHHHH
SNLCALGCRTTFISVVGDDGHGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLL
HHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEECCCHHH
RADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKGLFEGRTAPDVIARCRAARKP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHCCCC
VIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIEN
EEECCCCCCHHHHCCCEEECCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCHHH
LIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALV
EEEEEECCCEEEEECCCCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHH
VSNVAAGIVVGKFGTASVTGTELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREK
HHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
KVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDASIKRLKGEDRPINNEEMRSLL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
LASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLE
HHHHHHHHEEECCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCHHHHCCCCEEECCCCC
GFSSTNIINRMKSSPE
CCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVV
CCHHHHHHCCHHHEEEEEEHHHHHHHHHHCCHHHCCCCCCCCHHHCCCHHHHCCCCHHHH
SNLCALGCRTTFISVVGDDGHGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLL
HHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEECCCHHH
RADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKGLFEGRTAPDVIARCRAARKP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCHHHHHHHHHCCCC
VIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIEN
EEECCCCCCHHHHCCCEEECCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCHHH
LIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALV
EEEEEECCCEEEEECCCCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHH
VSNVAAGIVVGKFGTASVTGTELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREK
HHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
KVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDASIKRLKGEDRPINNEEMRSLL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
LASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLE
HHHHHHHHEEECCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCHHHHCCCCEEECCCCC
GFSSTNIINRMKSSPE
CCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA