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The map label for this gene is bsoBIM [H]

Identifier: 187734834

GI number: 187734834

Start: 382474

End: 383769

Strand: Reverse

Name: bsoBIM [H]

Synonym: Amuc_0325

Alternate gene names: 187734834

Gene position: 383769-382474 (Counterclockwise)

Preceding gene: 187734835

Following gene: 187734833

Centisome position: 14.41

GC content: 34.03

Gene sequence:

>1296_bases
ATGTACAAAGACTATAAATTCATAGATTGCACAGAAAAATACCATTCGGTTATGCAATTTGACAAAAACAAGAATGTTTT
TGTACATCGTTGGTACCCTTTTGTGGAAGGATATTCTAAGGAGTTTATTGAAGACATACTAGGCGAATTACCTTTTGCGC
CAACCTGTGCATTAGAGCCTTTCTGTGGTAGCGGAACAACTCCTGTTGAACTGCAAAACCACGGAATAAAGTGCTATTCT
TTTGAGGTCAGTCCTTTTATGCATCTCCTATCAACGGTCAAATTGGGTAGAAAATATAATGTCGATACCTTTACGTATTA
TGTTAAAGCCGTAACAAAGAAGCTGTCACGTACAAGTCGAAATATACGTAAAATAGAATCATTACCTTTTGGCGATACGA
TTGTAAAAAAAGAGCAGAGTAAGAAGTGGAATTTTCACGACACTGCTATAGATGGAATACTTGATGTAAGACATGCCATT
AGAACGATTGTTGATAATGATGATTATAAGAATTTATTTACAATCGCATTGGCTTCGATTATCATTCAAGCAAGCAATAT
GTTTCGTAATGGCAAGTGCTTGTCGTACAAGAAAGGATGGGAAACTAGAATCTTTAGTAGAAAAGATATTCATAATTTTT
TTCTGGACAGATTAAATTCAATTTTTACAGAAGATATTAGAATCATTTCCAAACAGAATCCATCTGTACAAAATTCTGAG
ATATGCTATTTGGGAGATGTTCGGAAAAACATACAACAGGTTCCAGATAGAGAAGTGGATTTGATTATTACTTCTCCACC
CTATCTAAACTCCAGAGATTACACGGATATTTATATGTTAGAGCTAAAAGTTTTACAATTAATTAACTCTTATGAGGAAC
TTCGTGAATTACGTAAAAACACATTACGTTCTCATGTTCAAGTTCCATATGGAAAAGTCTTTCCGATAGCAAATGAAAGA
CTTAAGAATAGCCTTCTAGAGATGTCTAATAAAGAGTTAAATACATGGAATACAGATATTACAAATATGATATGTGCCTA
TTTTGAGGATATGCAATTTTTATTCTCGGAATTTGCAAAGAAAATGCATAAAGGGGGTGTCATATATTTCAATGTGGCTA
ATTCTGCTTACTATGGAGTTGAAGTTCCTGTTGATTATATCATTGCTGACATAGCAGAATCTTGTGGATTTAAGGTTCGC
GAAATAAGAAAAGCGAGGGATTTAAAAACCAGCCCACAGCAAAGTGATAAAATCGGCAAACTAAGAGAAAGTGTAATTGT
GATTGATTACCAGTGA

Upstream 100 bases:

>100_bases
GATCAAATACCTAAGAAAGATAGACCAGCTTATTACAGACACATTAAATTAGATGATTTTAGAGTAATAAATGAACATCC
CAACTGAATAATTAACAACT

Downstream 100 bases:

>100_bases
TTTACTTCCAGATCTAGTTTGAATATAAATTTGGAAGTAAAGTTAATGATATTGGTCTCATTAGTGGGAGCTTTTAGAGC
TGGATGATAGGCTTGAACAT

Product: hypothetical protein

Products: NA

Alternate protein names: M.BsoBI; N(4)- cytosine-specific methyltransferase BsoBI [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MYKDYKFIDCTEKYHSVMQFDKNKNVFVHRWYPFVEGYSKEFIEDILGELPFAPTCALEPFCGSGTTPVELQNHGIKCYS
FEVSPFMHLLSTVKLGRKYNVDTFTYYVKAVTKKLSRTSRNIRKIESLPFGDTIVKKEQSKKWNFHDTAIDGILDVRHAI
RTIVDNDDYKNLFTIALASIIIQASNMFRNGKCLSYKKGWETRIFSRKDIHNFFLDRLNSIFTEDIRIISKQNPSVQNSE
ICYLGDVRKNIQQVPDREVDLIITSPPYLNSRDYTDIYMLELKVLQLINSYEELRELRKNTLRSHVQVPYGKVFPIANER
LKNSLLEMSNKELNTWNTDITNMICAYFEDMQFLFSEFAKKMHKGGVIYFNVANSAYYGVEVPVDYIIADIAESCGFKVR
EIRKARDLKTSPQQSDKIGKLRESVIVIDYQ

Sequences:

>Translated_431_residues
MYKDYKFIDCTEKYHSVMQFDKNKNVFVHRWYPFVEGYSKEFIEDILGELPFAPTCALEPFCGSGTTPVELQNHGIKCYS
FEVSPFMHLLSTVKLGRKYNVDTFTYYVKAVTKKLSRTSRNIRKIESLPFGDTIVKKEQSKKWNFHDTAIDGILDVRHAI
RTIVDNDDYKNLFTIALASIIIQASNMFRNGKCLSYKKGWETRIFSRKDIHNFFLDRLNSIFTEDIRIISKQNPSVQNSE
ICYLGDVRKNIQQVPDREVDLIITSPPYLNSRDYTDIYMLELKVLQLINSYEELRELRKNTLRSHVQVPYGKVFPIANER
LKNSLLEMSNKELNTWNTDITNMICAYFEDMQFLFSEFAKKMHKGGVIYFNVANSAYYGVEVPVDYIIADIAESCGFKVR
EIRKARDLKTSPQQSDKIGKLRESVIVIDYQ
>Mature_431_residues
MYKDYKFIDCTEKYHSVMQFDKNKNVFVHRWYPFVEGYSKEFIEDILGELPFAPTCALEPFCGSGTTPVELQNHGIKCYS
FEVSPFMHLLSTVKLGRKYNVDTFTYYVKAVTKKLSRTSRNIRKIESLPFGDTIVKKEQSKKWNFHDTAIDGILDVRHAI
RTIVDNDDYKNLFTIALASIIIQASNMFRNGKCLSYKKGWETRIFSRKDIHNFFLDRLNSIFTEDIRIISKQNPSVQNSE
ICYLGDVRKNIQQVPDREVDLIITSPPYLNSRDYTDIYMLELKVLQLINSYEELRELRKNTLRSHVQVPYGKVFPIANER
LKNSLLEMSNKELNTWNTDITNMICAYFEDMQFLFSEFAKKMHKGGVIYFNVANSAYYGVEVPVDYIIADIAESCGFKVR
EIRKARDLKTSPQQSDKIGKLRESVIVIDYQ

Specific function: This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsoBI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 50321; Mature: 50321

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKDYKFIDCTEKYHSVMQFDKNKNVFVHRWYPFVEGYSKEFIEDILGELPFAPTCALEP
CCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCC
FCGSGTTPVELQNHGIKCYSFEVSPFMHLLSTVKLGRKYNVDTFTYYVKAVTKKLSRTSR
CCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NIRKIESLPFGDTIVKKEQSKKWNFHDTAIDGILDVRHAIRTIVDNDDYKNLFTIALASI
HHHHHHCCCCCCHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IIQASNMFRNGKCLSYKKGWETRIFSRKDIHNFFLDRLNSIFTEDIRIISKQNPSVQNSE
HHHHHHHHCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ICYLGDVRKNIQQVPDREVDLIITSPPYLNSRDYTDIYMLELKVLQLINSYEELRELRKN
EEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSHVQVPYGKVFPIANERLKNSLLEMSNKELNTWNTDITNMICAYFEDMQFLFSEFAK
HHHHHCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KMHKGGVIYFNVANSAYYGVEVPVDYIIADIAESCGFKVREIRKARDLKTSPQQSDKIGK
HHHCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHH
LRESVIVIDYQ
HHCCEEEEEEC
>Mature Secondary Structure
MYKDYKFIDCTEKYHSVMQFDKNKNVFVHRWYPFVEGYSKEFIEDILGELPFAPTCALEP
CCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCC
FCGSGTTPVELQNHGIKCYSFEVSPFMHLLSTVKLGRKYNVDTFTYYVKAVTKKLSRTSR
CCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NIRKIESLPFGDTIVKKEQSKKWNFHDTAIDGILDVRHAIRTIVDNDDYKNLFTIALASI
HHHHHHCCCCCCHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IIQASNMFRNGKCLSYKKGWETRIFSRKDIHNFFLDRLNSIFTEDIRIISKQNPSVQNSE
HHHHHHHHCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ICYLGDVRKNIQQVPDREVDLIITSPPYLNSRDYTDIYMLELKVLQLINSYEELRELRKN
EEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRSHVQVPYGKVFPIANERLKNSLLEMSNKELNTWNTDITNMICAYFEDMQFLFSEFAK
HHHHHCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KMHKGGVIYFNVANSAYYGVEVPVDYIIADIAESCGFKVREIRKARDLKTSPQQSDKIGK
HHHCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHH
LRESVIVIDYQ
HHCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8917312 [H]