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The map label for this gene is kinA [H]

Identifier: 187734822

GI number: 187734822

Start: 366881

End: 368032

Strand: Direct

Name: kinA [H]

Synonym: Amuc_0311

Alternate gene names: 187734822

Gene position: 366881-368032 (Clockwise)

Preceding gene: 187734803

Following gene: 187734823

Centisome position: 13.77

GC content: 55.21

Gene sequence:

>1152_bases
ATGAAAAAAGACACCATTGACAAGCTGATCGGCCGCATTGACCACATCAAGGAGGAGGATCTCCAGCGCTTTTTTGTCAA
GCTGGCGGAACAGCAGGGTTTTTTCCAGCAGGTATTTGAAGCCATTCAGGAAGGCCTGATCCTGCTGGATAACAAGGGAA
AAATTCTGTTCGTCAACAAGGCTGCCCTTAAACTCTTTGACAAGGAACGCGGGCAAATAACCCCGGATGACTTCTGTATT
TTCCTGGGCAGGGACTGCTCATGGGACACGATCCAGCAGAGCCAGACCGCCGTCTCCCGGGACACGGAAATCTTTTACCC
GGAACACAGATTCCTGAACATTTTCATTTCCCCCATCGGCAGCAAAAACCAGGGCCACCTGGTCCTGATACGGGATGAAA
CGCCCCGGCATAAAAAAAACGCGGAAAACCTTGAGGCAGAACGCCTGAATGCCTTGACGCTGCTGGCCGCAGGAGTGGCT
CATGAAATAGGCAACCCCCTCAATTCCATAGGCCTCCATCTCCAGCTCCTTGCCAGGAAGGCAAAGCAGCTGCCTCCAAA
GTACCGGACGGACATGGAGGAATTGCTGAAAACAGCGGAGAGCGAAACCACGCGGCTGGACGTTATCCTGAAGCAATTCC
TCCAGGCCATCCGCCCCACCAGGCCCATCCGGGAACCCTACAACATTGAAACCATCCTCATGGAAGTGCTGAAACTGCTG
GAACCGGAAATCCAGCAGCGCGGCATCCAAATCAACACGGACCTCCAGCCCAGTCTTCCCATCCTCAGCCTGGACCCCGT
CCAAATCAAACAGGTCTTTTACAACCTGATCAAGAATGCCTACCAGTCCATCCCTCCGGAGGGAGGCACCATTCTGCTCA
AAAGCGGTTATACGGATGACAGCGTATTCGTCACTGTGGCGGATACCGGCTGCGGTATTTCACCGGAGGTCATGGGCAGC
ATTTATGAACCTTTCCTGACCACCAAGTCCACCGGCTCCGGACTGGGGCTGCTTATCGTTCGCCGCATCGTGAAGGAGCA
CGGCGGGTCCATCACGCTGGCCAGCCAGCCGGGTCAGGGAACCACCATCACGGTCTTCCTGCCGCGCGTGGAACGCACCA
TCAGGCTCCTGCCATCCTCCATTCCCTCATGA

Upstream 100 bases:

>100_bases
CCAGCCGCAAATGCGCGACAATGTGTCACATCAGCTTGATTTGTGACATGCCGCTCTTTATATATATAAGAGCAAGCCGC
ACGGCAACGGACCCGCCGCC

Downstream 100 bases:

>100_bases
AGATGCCTGTTCTGCTGATTGTAGATGATGAAAAACCCACGCGCGACGCTCTGCGCATGGGTTTTCAGGATGATTATGAA
GTGTACACCGCCGCCAATCT

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKKDTIDKLIGRIDHIKEEDLQRFFVKLAEQQGFFQQVFEAIQEGLILLDNKGKILFVNKAALKLFDKERGQITPDDFCI
FLGRDCSWDTIQQSQTAVSRDTEIFYPEHRFLNIFISPIGSKNQGHLVLIRDETPRHKKNAENLEAERLNALTLLAAGVA
HEIGNPLNSIGLHLQLLARKAKQLPPKYRTDMEELLKTAESETTRLDVILKQFLQAIRPTRPIREPYNIETILMEVLKLL
EPEIQQRGIQINTDLQPSLPILSLDPVQIKQVFYNLIKNAYQSIPPEGGTILLKSGYTDDSVFVTVADTGCGISPEVMGS
IYEPFLTTKSTGSGLGLLIVRRIVKEHGGSITLASQPGQGTTITVFLPRVERTIRLLPSSIPS

Sequences:

>Translated_383_residues
MKKDTIDKLIGRIDHIKEEDLQRFFVKLAEQQGFFQQVFEAIQEGLILLDNKGKILFVNKAALKLFDKERGQITPDDFCI
FLGRDCSWDTIQQSQTAVSRDTEIFYPEHRFLNIFISPIGSKNQGHLVLIRDETPRHKKNAENLEAERLNALTLLAAGVA
HEIGNPLNSIGLHLQLLARKAKQLPPKYRTDMEELLKTAESETTRLDVILKQFLQAIRPTRPIREPYNIETILMEVLKLL
EPEIQQRGIQINTDLQPSLPILSLDPVQIKQVFYNLIKNAYQSIPPEGGTILLKSGYTDDSVFVTVADTGCGISPEVMGS
IYEPFLTTKSTGSGLGLLIVRRIVKEHGGSITLASQPGQGTTITVFLPRVERTIRLLPSSIPS
>Mature_383_residues
MKKDTIDKLIGRIDHIKEEDLQRFFVKLAEQQGFFQQVFEAIQEGLILLDNKGKILFVNKAALKLFDKERGQITPDDFCI
FLGRDCSWDTIQQSQTAVSRDTEIFYPEHRFLNIFISPIGSKNQGHLVLIRDETPRHKKNAENLEAERLNALTLLAAGVA
HEIGNPLNSIGLHLQLLARKAKQLPPKYRTDMEELLKTAESETTRLDVILKQFLQAIRPTRPIREPYNIETILMEVLKLL
EPEIQQRGIQINTDLQPSLPILSLDPVQIKQVFYNLIKNAYQSIPPEGGTILLKSGYTDDSVFVTVADTGCGISPEVMGS
IYEPFLTTKSTGSGLGLLIVRRIVKEHGGSITLASQPGQGTTITVFLPRVERTIRLLPSSIPS

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=35, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788549, Length=341, Percent_Identity=28.4457478005865, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790300, Length=244, Percent_Identity=31.5573770491803, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081816, Length=230, Percent_Identity=31.7391304347826, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789808, Length=221, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=260, Percent_Identity=28.0769230769231, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=27.3913043478261, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786600, Length=363, Percent_Identity=25.068870523416, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=25.9911894273128, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788713, Length=119, Percent_Identity=33.6134453781513, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=26.1061946902655, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787894, Length=247, Percent_Identity=28.7449392712551, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43046; Mature: 43046

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDTIDKLIGRIDHIKEEDLQRFFVKLAEQQGFFQQVFEAIQEGLILLDNKGKILFVNK
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCEEEEEH
AALKLFDKERGQITPDDFCIFLGRDCSWDTIQQSQTAVSRDTEIFYPEHRFLNIFISPIG
HHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEEECCC
SKNQGHLVLIRDETPRHKKNAENLEAERLNALTLLAAGVAHEIGNPLNSIGLHLQLLARK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AKQLPPKYRTDMEELLKTAESETTRLDVILKQFLQAIRPTRPIREPYNIETILMEVLKLL
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EPEIQQRGIQINTDLQPSLPILSLDPVQIKQVFYNLIKNAYQSIPPEGGTILLKSGYTDD
CHHHHHCCEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
SVFVTVADTGCGISPEVMGSIYEPFLTTKSTGSGLGLLIVRRIVKEHGGSITLASQPGQG
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
TTITVFLPRVERTIRLLPSSIPS
CEEEEEECHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKDTIDKLIGRIDHIKEEDLQRFFVKLAEQQGFFQQVFEAIQEGLILLDNKGKILFVNK
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCEEEEEH
AALKLFDKERGQITPDDFCIFLGRDCSWDTIQQSQTAVSRDTEIFYPEHRFLNIFISPIG
HHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEEECCC
SKNQGHLVLIRDETPRHKKNAENLEAERLNALTLLAAGVAHEIGNPLNSIGLHLQLLARK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AKQLPPKYRTDMEELLKTAESETTRLDVILKQFLQAIRPTRPIREPYNIETILMEVLKLL
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EPEIQQRGIQINTDLQPSLPILSLDPVQIKQVFYNLIKNAYQSIPPEGGTILLKSGYTDD
CHHHHHCCEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
SVFVTVADTGCGISPEVMGSIYEPFLTTKSTGSGLGLLIVRRIVKEHGGSITLASQPGQG
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
TTITVFLPRVERTIRLLPSSIPS
CEEEEEECHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]