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The map label for this gene is bepF [H]

Identifier: 187734731

GI number: 187734731

Start: 273283

End: 274512

Strand: Direct

Name: bepF [H]

Synonym: Amuc_0219

Alternate gene names: 187734731

Gene position: 273283-274512 (Clockwise)

Preceding gene: 187734730

Following gene: 187734732

Centisome position: 10.26

GC content: 59.02

Gene sequence:

>1230_bases
ATGACGCGGACGGCGACGCCGCCCGGAAAACCTTCTTTCAACAAAGGCGCCCGCAGGCGTGTCATTCCCAGCATGGACTT
GACACGCCTGACGGGCTCTATCTCCAGCCCCGCCGTTTTCCTCTTCCTGCTTTCCCTCTTTTTTGCCGGGTGTAAAAAGA
CGGAACAGGTTCCGCATGAAACGCCCACGCTTATTTATGAACACCCCGTCACCATGGATATTCCGGTGACGGGCAGCTGG
GTGGGCCACCTGGACGGCGTGGACAACGCCTCCATCGTCCCCCAGATTACGGGCTACCTTCAAACGCAGAATTACGCCAA
TGGCGCTATTGTGAAGAAGGGGGAAGTACTCTTCCGCATTGACGATTCCACGTTCAGGGACCAGGTATCCAAGGCCAAAG
CCACCCTGGCCCAGGCACAGGCCCAGCTACAGCAGTTGAATTACGACGCGGAAATTTACAGGCCCCTGGCTGCGGAGAAT
GCCATTTCCAAGCAGAAATACGAGGATACGCGCCTGTCTGCTCTTGCCGCGGAAGCACAGGTGCAGCAGGCGGCGGCGGC
CCTGGCCCTGGCTGAAAAAAATCTTTCCTATACGGTGCTGTACGCGCCTTTTGACGGAATAGCCGGCATCTCCAGGACAA
ACATCGGCGACCTGGTCAGCCCGGAAAGCGGCCCGCTTGCCGTCGTCTCCTCCGTCAATCCCATCCGCGTCAATTTCGCC
GTCACCCAGCAGGAATGGATTCAGCAGGCGGGGAAAAACGGGAATGAAGGCGTCCAGACGGGCAGCAAGCTGGACATTAC
GCTGAAAGACGGCACCAAATACCCGGAACAGGCCACGGTAGTTGCCATTGACCGGGCGTTCAATCCCCAGACAGGCACCA
TCCGCGTACAGGCCAACCTTCCCAATGCGGACTATCTGCTGCGCCCCGGCATGTTCATCATCGCCACGGCCCGGCTGGCC
ACCGTCAAAAACGCGCTGACCGTTCCGGCAAAATCCCTTCTTTCGACACAGGGGCGCTTTTTCGTGATTGTTCTGGACGG
GAGCAACCACCCTTCCATCATCCCCGTACAGGCCGGGACGCAGCTTGGAGACCGGCAGCAGGTTATTCCTCTGAAACCGG
ATTCCCTCACCCCCCAAAGCAAAGTGGTCGTGGACGGTCTTCTCCAGGCGGAAGCAGCCTCCAGAAATCCGGCCGCCCGC
CTGAAAGCGGTACCCGCCAGCAACCAATAA

Upstream 100 bases:

>100_bases
GCACCTCCCTTGTCCTGTGGTTCGGAGAACTGGCGCGGGCATTCACTCCGGATAAATCCGACGCCCGCTCCTGAACCGTC
CGGGGGAATTCCCCCCGCGC

Downstream 100 bases:

>100_bases
GCTCCTCCCGCCATGGCTGATTTCTTCATCAAGCGCCCCATCCTTTCCATCTGCCTCTCCGTCATCATTGTCCTCCTGGG
GGCCTTTTCCATTCTGCGCC

Product: efflux transporter, RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRTATPPGKPSFNKGARRRVIPSMDLTRLTGSISSPAVFLFLLSLFFAGCKKTEQVPHETPTLIYEHPVTMDIPVTGSW
VGHLDGVDNASIVPQITGYLQTQNYANGAIVKKGEVLFRIDDSTFRDQVSKAKATLAQAQAQLQQLNYDAEIYRPLAAEN
AISKQKYEDTRLSALAAEAQVQQAAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFA
VTQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQANLPNADYLLRPGMFIIATARLA
TVKNALTVPAKSLLSTQGRFFVIVLDGSNHPSIIPVQAGTQLGDRQQVIPLKPDSLTPQSKVVVDGLLQAEAASRNPAAR
LKAVPASNQ

Sequences:

>Translated_409_residues
MTRTATPPGKPSFNKGARRRVIPSMDLTRLTGSISSPAVFLFLLSLFFAGCKKTEQVPHETPTLIYEHPVTMDIPVTGSW
VGHLDGVDNASIVPQITGYLQTQNYANGAIVKKGEVLFRIDDSTFRDQVSKAKATLAQAQAQLQQLNYDAEIYRPLAAEN
AISKQKYEDTRLSALAAEAQVQQAAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFA
VTQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQANLPNADYLLRPGMFIIATARLA
TVKNALTVPAKSLLSTQGRFFVIVLDGSNHPSIIPVQAGTQLGDRQQVIPLKPDSLTPQSKVVVDGLLQAEAASRNPAAR
LKAVPASNQ
>Mature_408_residues
TRTATPPGKPSFNKGARRRVIPSMDLTRLTGSISSPAVFLFLLSLFFAGCKKTEQVPHETPTLIYEHPVTMDIPVTGSWV
GHLDGVDNASIVPQITGYLQTQNYANGAIVKKGEVLFRIDDSTFRDQVSKAKATLAQAQAQLQQLNYDAEIYRPLAAENA
ISKQKYEDTRLSALAAEAQVQQAAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFAV
TQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQANLPNADYLLRPGMFIIATARLAT
VKNALTVPAKSLLSTQGRFFVIVLDGSNHPSIIPVQAGTQLGDRQQVIPLKPDSLTPQSKVVVDGLLQAEAASRNPAARL
KAVPASNQ

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=27.4932614555256, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1786668, Length=356, Percent_Identity=28.9325842696629, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789929, Length=325, Percent_Identity=25.8461538461538, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082036, Length=319, Percent_Identity=26.3322884012539, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43910; Mature: 43778

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTATPPGKPSFNKGARRRVIPSMDLTRLTGSISSPAVFLFLLSLFFAGCKKTEQVPHE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHCCCCC
TPTLIYEHPVTMDIPVTGSWVGHLDGVDNASIVPQITGYLQTQNYANGAIVKKGEVLFRI
CCCEEEECCEEEEECCCCCCEECCCCCCCCCCCHHHHHEEEECCCCCCEEEECCCEEEEE
DDSTFRDQVSKAKATLAQAQAQLQQLNYDAEIYRPLAAENAISKQKYEDTRLSALAAEAQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQQAAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEE
VTQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQANL
ECHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECC
PNADYLLRPGMFIIATARLATVKNALTVPAKSLLSTQGRFFVIVLDGSNHPSIIPVQAGT
CCCCEEECCCEEEEEHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCC
QLGDRQQVIPLKPDSLTPQSKVVVDGLLQAEAASRNPAARLKAVPASNQ
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCC
>Mature Secondary Structure 
TRTATPPGKPSFNKGARRRVIPSMDLTRLTGSISSPAVFLFLLSLFFAGCKKTEQVPHE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHCCCCC
TPTLIYEHPVTMDIPVTGSWVGHLDGVDNASIVPQITGYLQTQNYANGAIVKKGEVLFRI
CCCEEEECCEEEEECCCCCCEECCCCCCCCCCCHHHHHEEEECCCCCCEEEECCCEEEEE
DDSTFRDQVSKAKATLAQAQAQLQQLNYDAEIYRPLAAENAISKQKYEDTRLSALAAEAQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQQAAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEE
VTQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQANL
ECHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECC
PNADYLLRPGMFIIATARLATVKNALTVPAKSLLSTQGRFFVIVLDGSNHPSIIPVQAGT
CCCCEEECCCEEEEEHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCC
QLGDRQQVIPLKPDSLTPQSKVVVDGLLQAEAASRNPAARLKAVPASNQ
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]