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The map label for this gene is sepA [H]

Identifier: 187734622

GI number: 187734622

Start: 136322

End: 137698

Strand: Reverse

Name: sepA [H]

Synonym: Amuc_0108

Alternate gene names: 187734622

Gene position: 137698-136322 (Counterclockwise)

Preceding gene: 187734623

Following gene: 187734621

Centisome position: 5.17

GC content: 58.17

Gene sequence:

>1377_bases
ATGAAGCATGCTTCAATAATATTGTTAACCAACAACATGAAAGCACACTATATACTGATAGCGGCCCTGTGCGGGCTGGC
AGGGTGCCGGGAAGACAACGGCGCTGCGGATGCTGCAAACCGGAGGGAAATGCCTCCGCTCAAGGTTTCCGTGATGAATA
TGGAGCAGAGAACGGTGGAGTTGAAATCTTCCTGGTTCGGCCATTTGCGCGGGGTGGAGCAGGCGGACATCAGGCCGCAA
GTTTCCGGAACGCTGATACGCCAGGTTTACTGGGACGGCTCCATTTGCAACAAGGGAGAACTGCTCTTTGAAATTGACCC
GGCTACCTATCAGGCCGCCGTGGACAGGGAGGCGGCCAACCTGGCTACGGCTCGTGCCGTCAAACTTCAGGCGGAAGCTT
CTCTGAACAGGGTGCAGAAGGATTTGGACCGCTATGACAAACTGATCAGGACGGGGGCTATTTCCGTCAAGAACCTGACG
GACGCGCAACAGAACCTTAAGGAGATGCAGGCTGCTCTGGAAGAGGCGGAAGCGGGCATCAAGCAGGCGGAAGCCTCCCT
GGAAAACGCCCGGATCAATCTGGACCGCACCAAAGTACGCGCGCCGTTCCGGGGACTGGCTTCCAAGGCGACGGCCAGCG
TGGGGGAACTGATTTCCGCCAGCGGCTCGCCGCTCACTACCATGTCTTCCATCGACCCCATCCGGGTGGATTTTTCCGTG
ACGGGAAAACAGGTTCTCAGCCGTCTGATGGATGGAACCGTGAATGTGAAAACCGGGCGCATGGGAGACATGCCGGATTT
TGAACTGATTCTGGAAGACGGCTCCGTCTTTGAAAAGAAGGGGAAGGTGGTTTCCGTGGACAGCGAGGTGAGCCGTTCGA
CGGGCACCGTCAATTTCATCGGCCATATTCCCAATCCGGATCTGAAACTGCGTTCCGGAATGGCGGTGCGCGTGCGCGCC
GTGACGGGTACGGAGAAGGATGCCTTGCTGGTTCCCGCCCGCGCCCTGCTTTCTTCCATGAACCACCGCAACATCATGGT
GGTGGCTCCGGACGGGACGCCGCGCCGCATTGACGTGCAGCCGGGGGAAACCGTAGTCCTTGACATGCCTGACGGCAAGG
GCGGAACGGCCCCCATGCTGATGCAGATAGTGACGGGAACGGTCAAGCCCATTCCGGAATCTCTGAAGGAAATAGGATTC
GGAAAGCCGGCGGATGCTCCCGTCATTGTGGAAGGCGGCATGATGGCCGCCCAGTATTCCAAGGCGAACAGCCTGATGCG
GGAACATGGTGCCACGGAAGGGTTCGGGACGGTTGTCCCCGTTCCCTTCATCTATACCGCCCCTGTTTCCACCACTCCTT
CCGTAACTGCCAAATAA

Upstream 100 bases:

>100_bases
GGAAAGCCGCAGCATGCTGAGGGATCTGCTCCGGCGCATGTACGACAATCTCAAATAATAAAAGCCCGTGCTTTTTTCCG
GGGAACGGGTTTGGAAAAAT

Downstream 100 bases:

>100_bases
GCCATGAGTGCGTTTTTCATCAAGCATCCCGCAATCGCAGCCGTGATTGCCATTGTGACCACGCTGCTGGGGCTGGTCTG
CATGTTCAACCTGCCTATTT

Product: efflux transporter, RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKHASIILLTNNMKAHYILIAALCGLAGCREDNGAADAANRREMPPLKVSVMNMEQRTVELKSSWFGHLRGVEQADIRPQ
VSGTLIRQVYWDGSICNKGELLFEIDPATYQAAVDREAANLATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLT
DAQQNLKEMQAALEEAEAGIKQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSSIDPIRVDFSV
TGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDSEVSRSTGTVNFIGHIPNPDLKLRSGMAVRVRA
VTGTEKDALLVPARALLSSMNHRNIMVVAPDGTPRRIDVQPGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGF
GKPADAPVIVEGGMMAAQYSKANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK

Sequences:

>Translated_458_residues
MKHASIILLTNNMKAHYILIAALCGLAGCREDNGAADAANRREMPPLKVSVMNMEQRTVELKSSWFGHLRGVEQADIRPQ
VSGTLIRQVYWDGSICNKGELLFEIDPATYQAAVDREAANLATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLT
DAQQNLKEMQAALEEAEAGIKQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSSIDPIRVDFSV
TGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDSEVSRSTGTVNFIGHIPNPDLKLRSGMAVRVRA
VTGTEKDALLVPARALLSSMNHRNIMVVAPDGTPRRIDVQPGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGF
GKPADAPVIVEGGMMAAQYSKANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK
>Mature_458_residues
MKHASIILLTNNMKAHYILIAALCGLAGCREDNGAADAANRREMPPLKVSVMNMEQRTVELKSSWFGHLRGVEQADIRPQ
VSGTLIRQVYWDGSICNKGELLFEIDPATYQAAVDREAANLATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLT
DAQQNLKEMQAALEEAEAGIKQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSSIDPIRVDFSV
TGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDSEVSRSTGTVNFIGHIPNPDLKLRSGMAVRVRA
VTGTEKDALLVPARALLSSMNHRNIMVVAPDGTPRRIDVQPGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGF
GKPADAPVIVEGGMMAAQYSKANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=336, Percent_Identity=31.8452380952381, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789929, Length=324, Percent_Identity=29.6296296296296, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786668, Length=342, Percent_Identity=30.4093567251462, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87082036, Length=298, Percent_Identity=26.8456375838926, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081796, Length=274, Percent_Identity=27.007299270073, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081951, Length=176, Percent_Identity=30.1136363636364, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790012, Length=280, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790024, Length=178, Percent_Identity=28.6516853932584, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49204; Mature: 49204

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHASIILLTNNMKAHYILIAALCGLAGCREDNGAADAANRREMPPLKVSVMNMEQRTVE
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHH
LKSSWFGHLRGVEQADIRPQVSGTLIRQVYWDGSICNKGELLFEIDPATYQAAVDREAAN
HHHHHHHHHCCCCHHCCCCCHHHHEEEHHEECCCCCCCCCEEEEECCCHHHHHHHHHHHH
LATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLTDAQQNLKEMQAALEEAEAGI
HHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSSIDPIRVDFSV
HHHHHHHHHCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEE
TGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDSEVSRSTGTVNFI
CHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCEEHHCCCEEEECCHHCCCCCEEEEE
GHIPNPDLKLRSGMAVRVRAVTGTEKDALLVPARALLSSMNHRNIMVVAPDGTPRRIDVQ
ECCCCCCEEECCCCEEEEEEEECCCCCEEEECHHHHHHCCCCCEEEEECCCCCCCEEECC
PGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGFGKPADAPVIVEGGMMAAQYS
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEECCEEEHHHH
KANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK
HHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKHASIILLTNNMKAHYILIAALCGLAGCREDNGAADAANRREMPPLKVSVMNMEQRTVE
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHH
LKSSWFGHLRGVEQADIRPQVSGTLIRQVYWDGSICNKGELLFEIDPATYQAAVDREAAN
HHHHHHHHHCCCCHHCCCCCHHHHEEEHHEECCCCCCCCCEEEEECCCHHHHHHHHHHHH
LATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLTDAQQNLKEMQAALEEAEAGI
HHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSSIDPIRVDFSV
HHHHHHHHHCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEE
TGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDSEVSRSTGTVNFI
CHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCEEHHCCCEEEECCHHCCCCCEEEEE
GHIPNPDLKLRSGMAVRVRAVTGTEKDALLVPARALLSSMNHRNIMVVAPDGTPRRIDVQ
ECCCCCCEEECCCCEEEEEEEECCCCCEEEECHHHHHHCCCCCEEEEECCCCCCCEEECC
PGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGFGKPADAPVIVEGGMMAAQYS
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEECCEEEHHHH
KANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK
HHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]