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The map label for this gene is ispDF [H]

Identifier: 187734582

GI number: 187734582

Start: 91155

End: 91850

Strand: Direct

Name: ispDF [H]

Synonym: Amuc_0068

Alternate gene names: 187734582

Gene position: 91155-91850 (Clockwise)

Preceding gene: 187734581

Following gene: 187734583

Centisome position: 3.42

GC content: 57.47

Gene sequence:

>696_bases
ATGAAATGCTGCGCCGCCATCATCGTTGCGGCCGGGTCCTCCCGGCGTGCCGGATTTGACAAGCTGCTGGCCCCCCTGCA
CGGCGTCAGGGTGCTGGAACGCAGCATCCGCGCCTTTGCCAACTGCCGGGAAATCACGGAAATCGTAGTCGTTTGCCCGG
AGGAACGTTTTCATGCCATTAACGGCGCAAACCTGGAAACGGAAATACCCGTTACCCGTGTGGACGGAGGGGCGGAACGG
CATGAATCCGTGCAGAACGGACTGGCAGCCCTGCTTTATACCCCGGAATTCGTGGCCGTGCATGATGGAGCCCGCCCGCT
CATTACGGTGGAGCAAATCTCCCGCTGCATTCAAACCGCCAGGGAATATGGGGCGTCCGCTTCCGCACATCCCGTAACGG
ACACCCTGAAGCGTGCGGACAAAGAACACTTTACGCTTCCGGAACAGGTGGAACGGGACGGGCTGTGGTGCATGGAAACC
CCGCAGGTCTTTCAATATCCTCTTCTCCTGGACGCCTATGTGGAAGTCACGGAACGGAATATTCAGGTAACGGATGAAGT
AACCGCCCTGCAACTCATCGGCCATCCTACGCGCCTGGTGCATAATCATGAGCCCAATCCCAAAATCACATGGCCGGAAG
ATATTTCCCGCGCTGAAATGCTAATGGAACTCAAGCACCTCCGCAATGACTCATGA

Upstream 100 bases:

>100_bases
CCAAAAAAGATATGGGGCAGGTCATGAAACTCATGCAGGAACGCACTGCCGGCAGAGTCAACGGCAAGGAGTTGGCCCGC
ATGGTGTCAGCCAGACTGTC

Downstream 100 bases:

>100_bases
CCGGCCTTCTGTGTAAGAATCCCCGCATCGTTCTCATTCTGGGAACAGGGTATCTGGGAAAAGCCCTGGCGGAAAGCCTG
CGGAAAGCGGGACATACGGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCREITEIVVVCPEERFHAINGANLETEIPVTRVDGGAER
HESVQNGLAALLYTPEFVAVHDGARPLITVEQISRCIQTAREYGASASAHPVTDTLKRADKEHFTLPEQVERDGLWCMET
PQVFQYPLLLDAYVEVTERNIQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRAEMLMELKHLRNDS

Sequences:

>Translated_231_residues
MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCREITEIVVVCPEERFHAINGANLETEIPVTRVDGGAER
HESVQNGLAALLYTPEFVAVHDGARPLITVEQISRCIQTAREYGASASAHPVTDTLKRADKEHFTLPEQVERDGLWCMET
PQVFQYPLLLDAYVEVTERNIQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRAEMLMELKHLRNDS
>Mature_231_residues
MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCREITEIVVVCPEERFHAINGANLETEIPVTRVDGGAER
HESVQNGLAALLYTPEFVAVHDGARPLITVEQISRCIQTAREYGASASAHPVTDTLKRADKEHFTLPEQVERDGLWCMET
PQVFQYPLLLDAYVEVTERNIQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRAEMLMELKHLRNDS

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=25, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=34.375, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 25849; Mature: 25849

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCREITEIVVVCPEERFHAI
CCHHHEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHC
NGANLETEIPVTRVDGGAERHESVQNGLAALLYTPEFVAVHDGARPLITVEQISRCIQTA
CCCCCCCCCCEEECCCCHHHHHHHHHHHEEEEECCCEEEEECCCCCEEEHHHHHHHHHHH
REYGASASAHPVTDTLKRADKEHFTLPEQVERDGLWCMETPQVFQYPLLLDAYVEVTERN
HHHCCCCCCCCHHHHHHHCCHHCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCC
IQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRAEMLMELKHLRNDS
EEECHHHHHHHHHCCCHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCREITEIVVVCPEERFHAI
CCHHHEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHC
NGANLETEIPVTRVDGGAERHESVQNGLAALLYTPEFVAVHDGARPLITVEQISRCIQTA
CCCCCCCCCCEEECCCCHHHHHHHHHHHEEEEECCCEEEEECCCCCEEEHHHHHHHHHHH
REYGASASAHPVTDTLKRADKEHFTLPEQVERDGLWCMETPQVFQYPLLLDAYVEVTERN
HHHCCCCCCCCHHHHHHHCCHHCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCC
IQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRAEMLMELKHLRNDS
EEECHHHHHHHHHCCCHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA