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The map label for this gene is pspE [H]

Identifier: 187734577

GI number: 187734577

Start: 86369

End: 86710

Strand: Reverse

Name: pspE [H]

Synonym: Amuc_0063

Alternate gene names: 187734577

Gene position: 86710-86369 (Counterclockwise)

Preceding gene: 187734578

Following gene: 187734567

Centisome position: 3.25

GC content: 58.19

Gene sequence:

>342_bases
ATGGTGACCGGGCTGACGGCCCTCTTCGTTTGGCTGAAAGGCAGGAATGCGGAACGTGACCCTGCTTCCTCCGCCATGGT
TGTGTCTCCGGAAGGCGCCGCCGCGCGTCCTGTTGTGCTGGTGGATGTCCGGACTCTGGATGAGTACCGCACGGGACATA
AGGAGGGCGCCTTGAATATTCCGGCAGATGAGGTGGAACAACTGGCCCCCCGGCTTCTTCCCGATAAGAACGCCGTGATT
ATGCTGTATTGCCGTACGGGAAAGAGAGCGGACAAGGCGCTGGAAGCGCTCAGGAAGATGGGTTATTCCCATGTGGAAAA
CCTGCACCGGTTCGAAATGTAA

Upstream 100 bases:

>100_bases
AATGACGCCTGCGCATGTGGGCAGGGCGTTGTTTTCTCTTGATTACTTCGCCGCGGTTTAGTGAAGTGGCCGCGTGAAAA
ACGTGGTTTTGTACGTGGTC

Downstream 100 bases:

>100_bases
CTTTCCGCCCGATTGTTCCGTGCGGGGATTCCGCAAGGCGCCGGGCATGCTGTTTCCTGGTTCGGACGGCGGAATTTTCT
AACGTAAAAGGCACGGAATG

Product: Rhodanese domain protein

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MVTGLTALFVWLKGRNAERDPASSAMVVSPEGAAARPVVLVDVRTLDEYRTGHKEGALNIPADEVEQLAPRLLPDKNAVI
MLYCRTGKRADKALEALRKMGYSHVENLHRFEM

Sequences:

>Translated_113_residues
MVTGLTALFVWLKGRNAERDPASSAMVVSPEGAAARPVVLVDVRTLDEYRTGHKEGALNIPADEVEQLAPRLLPDKNAVI
MLYCRTGKRADKALEALRKMGYSHVENLHRFEM
>Mature_113_residues
MVTGLTALFVWLKGRNAERDPASSAMVVSPEGAAARPVVLVDVRTLDEYRTGHKEGALNIPADEVEQLAPRLLPDKNAVI
MLYCRTGKRADKALEALRKMGYSHVENLHRFEM

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=67, Percent_Identity=43.2835820895522, Blast_Score=70, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 12546; Mature: 12546

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTGLTALFVWLKGRNAERDPASSAMVVSPEGAAARPVVLVDVRTLDEYRTGHKEGALNI
CCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCCCC
PADEVEQLAPRLLPDKNAVIMLYCRTGKRADKALEALRKMGYSHVENLHRFEM
CHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MVTGLTALFVWLKGRNAERDPASSAMVVSPEGAAARPVVLVDVRTLDEYRTGHKEGALNI
CCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECHHHHHHCCCCCCCCCC
PADEVEQLAPRLLPDKNAVIMLYCRTGKRADKALEALRKMGYSHVENLHRFEM
CHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]