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The map label for this gene is rsrIR [H]

Identifier: 187734576

GI number: 187734576

Start: 85349

End: 86056

Strand: Direct

Name: rsrIR [H]

Synonym: Amuc_0062

Alternate gene names: 187734576

Gene position: 85349-86056 (Clockwise)

Preceding gene: 187734575

Following gene: 187734579

Centisome position: 3.2

GC content: 39.12

Gene sequence:

>708_bases
ATGTCTCATAAAATTGCATTGCGTACCCAGCGGGAAGGTACAATTATTAATACTACTTCATCCAAGCAGGAAAAATTATT
ACGTCAAGCTGTGAAATCTGTTTCTATGCGTCTGAAAAAGAAATTTCCCGGAATCACCCTTGAATGGGAACCACAAGCTA
AATTGATAGATATTATTGTCCACCTTCGAACCCAATTCCCTGATGTTGACTTTGCAGCCCCCTCGTGCGCTTCTTCAATG
AAACCAGACGGTGGAATTCTTTATATGAAAAGTGAAGATGAACGGCGATTTCCTATCCTCATATCAGAAAAGAAAAACCA
AGGTACAAATGATATGAGGGCAAAGGAAGGAAAAAAAAGACAAGCTCAGGGGAATGCCATAGAAAGATTAGGAAAAAACG
TCATTGGTTTCCGAGCTTCCATGATTAGAGAGTCAATTTTTCCCTTTGTTTGTTTTGGAGATGGTTGTGATTTCGTTGAA
GGTTCATCCATAAGAGACCGTGTCATCACCATAGCCATGTTCGGTCAGCTCAACAAAGAATATGTTTATGCTCAAGGTCG
AGGAGAACTTTTTAACAGGGGATCTTTCTATTTCCGAGAAAAACAATGGACTGAACAAGAAATGGCAGACATCTCGTTCT
CTATAGCCAGCAAAGCCATCTTCTATTATTTTTCCAGATATGGAGAATCAACTTTTTTCGATAATTGA

Upstream 100 bases:

>100_bases
GATTATAACACATTCAGAGGGTGCAGAAATCTTAACGAACGCCCAATAAAAGTTAAGGAATTCCTCTTCCTTGTCGAAAA
ATTCTAATAGCCATCTCACT

Downstream 100 bases:

>100_bases
TTGGAGCACATTTTTAAAATTATTCAAAAAATATTCTAGATTTTAATATTTAGCAAATTACTCAGATGAAAAAACAGAGA
AGCTCAATAAAACTAGACTA

Product: hypothetical protein

Products: NA

Alternate protein names: R.RsrI; Endonuclease RsrI; Type II restriction enzyme RsrI [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSHKIALRTQREGTIINTTSSKQEKLLRQAVKSVSMRLKKKFPGITLEWEPQAKLIDIIVHLRTQFPDVDFAAPSCASSM
KPDGGILYMKSEDERRFPILISEKKNQGTNDMRAKEGKKRQAQGNAIERLGKNVIGFRASMIRESIFPFVCFGDGCDFVE
GSSIRDRVITIAMFGQLNKEYVYAQGRGELFNRGSFYFREKQWTEQEMADISFSIASKAIFYYFSRYGESTFFDN

Sequences:

>Translated_235_residues
MSHKIALRTQREGTIINTTSSKQEKLLRQAVKSVSMRLKKKFPGITLEWEPQAKLIDIIVHLRTQFPDVDFAAPSCASSM
KPDGGILYMKSEDERRFPILISEKKNQGTNDMRAKEGKKRQAQGNAIERLGKNVIGFRASMIRESIFPFVCFGDGCDFVE
GSSIRDRVITIAMFGQLNKEYVYAQGRGELFNRGSFYFREKQWTEQEMADISFSIASKAIFYYFSRYGESTFFDN
>Mature_234_residues
SHKIALRTQREGTIINTTSSKQEKLLRQAVKSVSMRLKKKFPGITLEWEPQAKLIDIIVHLRTQFPDVDFAAPSCASSMK
PDGGILYMKSEDERRFPILISEKKNQGTNDMRAKEGKKRQAQGNAIERLGKNVIGFRASMIRESIFPFVCFGDGCDFVEG
SSIRDRVITIAMFGQLNKEYVYAQGRGELFNRGSFYFREKQWTEQEMADISFSIASKAIFYYFSRYGESTFFDN

Specific function: Recognizes the double-stranded sequence GAATTC and cleaves after G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR004221
- InterPro:   IPR011336
- InterPro:   IPR018131
- ProDom:   PD039825 [H]

Pfam domain/function: PF02963 EcoRI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 26955; Mature: 26824

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKIALRTQREGTIINTTSSKQEKLLRQAVKSVSMRLKKKFPGITLEWEPQAKLIDIIV
CCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
HLRTQFPDVDFAAPSCASSMKPDGGILYMKSEDERRFPILISEKKNQGTNDMRAKEGKKR
HHHHCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCHH
QAQGNAIERLGKNVIGFRASMIRESIFPFVCFGDGCDFVEGSSIRDRVITIAMFGQLNKE
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHEEEEHHCCCCCE
YVYAQGRGELFNRGSFYFREKQWTEQEMADISFSIASKAIFYYFSRYGESTFFDN
EEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SHKIALRTQREGTIINTTSSKQEKLLRQAVKSVSMRLKKKFPGITLEWEPQAKLIDIIV
CCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
HLRTQFPDVDFAAPSCASSMKPDGGILYMKSEDERRFPILISEKKNQGTNDMRAKEGKKR
HHHHCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCHH
QAQGNAIERLGKNVIGFRASMIRESIFPFVCFGDGCDFVEGSSIRDRVITIAMFGQLNKE
HHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHEEEEHHCCCCCE
YVYAQGRGELFNRGSFYFREKQWTEQEMADISFSIASKAIFYYFSRYGESTFFDN
EEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695392; 2843805 [H]