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The map label for this gene is ubiE [H]

Identifier: 187734563

GI number: 187734563

Start: 63997

End: 64668

Strand: Direct

Name: ubiE [H]

Synonym: Amuc_0047

Alternate gene names: 187734563

Gene position: 63997-64668 (Clockwise)

Preceding gene: 187734560

Following gene: 187734564

Centisome position: 2.4

GC content: 59.67

Gene sequence:

>672_bases
ATGCGTAATCCAGAATTCGTCAAAGCCGCTTTTTCCGCTATTGCCCCGCGCTATGTGGCTACCAACCATGTGCTGAGCAT
GGGTGTGGACCTGCTATGGCGCCAGCGCGTCGTTCAGCTTGTCTCCGAATGGAAACCGGAAAACCTTCTGGATCTGGCGA
CTGGAACGGGAGACCTGGCCCTGGCCATCCTGAACGCAATGCCGGAAATCCGCCTCACCGGGTCCGACTTCTGCCAGCCC
ATGCTGGACATAGCGGCCAGGCGGGGACTGGATCATCTGGTTTGCGCAGACGCCATGAACCTGCCCTTCCCCTCCGCCTC
CTATGATGCGGTCACTGTCGCCTTTGGCCTGAGGAACATGGCCAGCTATCCGGACGCCCTGCGGGAAATGGGCCGTATGC
TGCGTCCGGGGGGCCATCTGCTCATTCTGGATTTTTCCCTGCCTGAGAGCCTGTTGAAAAGCCCTTACCGGTTTTATCTG
CACCGCATCCTTCCCGTCATCGCAGGGTGGATGACCGGCCACCGGGAAGCATACGACTACCTGGCGGACAGCATTGAAGC
CTTCCCCTGCGGGCAGGCCATGAAGACTCTGCTGCGGGAATGCGGCTACAGCAACATCACGGCGGAAGCTCTGAACGGGG
GAATTGCCAGCATTTACACAGCCCAGCGATGA

Upstream 100 bases:

>100_bases
GGTCGTTGCGGGTTCGAGTCCCGTCCGACACCCCATCCCCTTTCCTTCATAAAAAGAGCACTTATAGTGCTCTTTTTTAC
TATCCCCCAGCCCTTTCCGC

Downstream 100 bases:

>100_bases
GTGCGGGGGACGCAAGAGAAGGCATCATACGCGGCAAGGCGGCACGCGCCCGCCTGGGCGGAAAACTTGCCGGACTATCC
CTGCCCCGGCAAATCGCCGT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEWKPENLLDLATGTGDLALAILNAMPEIRLTGSDFCQP
MLDIAARRGLDHLVCADAMNLPFPSASYDAVTVAFGLRNMASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYL
HRILPVIAGWMTGHREAYDYLADSIEAFPCGQAMKTLLRECGYSNITAEALNGGIASIYTAQR

Sequences:

>Translated_223_residues
MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEWKPENLLDLATGTGDLALAILNAMPEIRLTGSDFCQP
MLDIAARRGLDHLVCADAMNLPFPSASYDAVTVAFGLRNMASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYL
HRILPVIAGWMTGHREAYDYLADSIEAFPCGQAMKTLLRECGYSNITAEALNGGIASIYTAQR
>Mature_223_residues
MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEWKPENLLDLATGTGDLALAILNAMPEIRLTGSDFCQP
MLDIAARRGLDHLVCADAMNLPFPSASYDAVTVAFGLRNMASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYL
HRILPVIAGWMTGHREAYDYLADSIEAFPCGQAMKTLLRECGYSNITAEALNGGIASIYTAQR

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=257, Percent_Identity=28.4046692607004, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI153251913, Length=119, Percent_Identity=34.453781512605, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI48994983, Length=228, Percent_Identity=31.140350877193, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557039, Length=226, Percent_Identity=36.7256637168142, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323526, Length=239, Percent_Identity=28.4518828451883, Blast_Score=102, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24641702, Length=237, Percent_Identity=33.7552742616034, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24680; Mature: 24680

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEWKPENLLDLATGTGDLA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHH
LAILNAMPEIRLTGSDFCQPMLDIAARRGLDHLVCADAMNLPFPSASYDAVTVAFGLRNM
HHHHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYLHRILPVIAGWMTGHREAYDY
HCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHH
LADSIEAFPCGQAMKTLLRECGYSNITAEALNGGIASIYTAQR
HHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEWKPENLLDLATGTGDLA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHH
LAILNAMPEIRLTGSDFCQPMLDIAARRGLDHLVCADAMNLPFPSASYDAVTVAFGLRNM
HHHHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYLHRILPVIAGWMTGHREAYDY
HCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHH
LADSIEAFPCGQAMKTLLRECGYSNITAEALNGGIASIYTAQR
HHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA