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The map label for this gene is ligA

Identifier: 187734527

GI number: 187734527

Start: 13898

End: 16282

Strand: Direct

Name: ligA

Synonym: Amuc_0011

Alternate gene names: 187734527

Gene position: 13898-16282 (Clockwise)

Preceding gene: 187734526

Following gene: 187734528

Centisome position: 0.52

GC content: 61.97

Gene sequence:

>2385_bases
ATGGAGGAAGACCTTTTTTCCCTGGCAGCCGGCAAACAGCCTTCACAGCAGGCAACAAATGAAACGGCACCCCGGGCCGG
GGAGGCGCGGGAAAATGCCGGCACCGACCACCCCGGAAACGCGGAGGACCCCGCTCACCGCCGCATAGACTATTTGCGTT
CCGAACTGCGCCGCCACAACCGTCTGTATTACGAACAGGCGGAACCGGAGATTTCCGACGCGGAATATGACGCCCTGTTC
CTGGAGCTGGAAAAATTGGAAAAAGCCCATCCCGAACTGTCGGATCCGGACTCCCCCACGCGCCGCGTAGGCGGCGCCCC
TCTCCAGGGCTTCAACCAGATCAGACATGCGGTGCCCATGTTGTCTATTGACGACATTTTTGAGCAGCGGGACGCCCCCG
TACCGGATGAAGAACTGGCGGAATTCTACCATAAGCTGTCCAGGGCGCTGCAAACGGAGAACGTGCCCGTCTCCGTTGAG
CCCAAGATTGACGGAGTGGCCCTTTCCATCATGTACCGCAATGGGAAGCTGGCTTATGCGGCCACGCGCGGAGACGGAGA
CGTGGGGGACGACGTAACGGCCAACGTCCGCACCATCCGCAGCGTCCCCCTCACCCTCCCCCCCGGCGCGCCGCCCGTGC
TGGAAGTGCGCGGGGAAGTCTTCATGCCCAATGAAGCCTTTGCCAAACTGAATGAAGAACGGGACGCCGACGGGCTCCCC
GCATTCGCCAACCCGCGCAACGCCACCGCCGGAACCCTCAAGCAGCTGGACCCTCGGCAAGTGGCCGCCCGGCCGCTGGC
CTTCCTGGCCCACGGATTGGGCGCGTATGAAGGCCCGGAACTCAGGGACGCAAAAGACTTCTGGAACATGCTCCGCCATT
GCGGCATCCCCTGCAATGAGCCGGTGTATTACACGGACAGCCTGGAATCCACCCGCCAGGCCGTGCGGGATATTGACAGG
CTCCGCCATACGCTCCCCTACGGCACGGACGGAGCCGTTATTAAAATCAGCTCCACGGCTACGCGGGAAGCGCTGGGAGC
TACGGCTCGCGCGCCCCGCTGGGCGGCGGCGTATAAATTCCCCCCGGAACAGAAGGAAACCACCCTGCTGAATATTGTCG
TGCAGGTAGGACGCACCGGGGTGCTGACACCTGTGGCGGAATTGCAGCCTGTGCTGCTGTCCGGTTCTACGGTAGCCCGG
GCCACCCTCCACAACCAGGATGAAATCGACCGGAAGGACGTTCGCATAGGCGATACGGTGCTGGTGGAAAAGGCGGGGGA
AATCATCCCGGCCGTTCTGAAAGTGAACCTTTCCAAACGCCCGCAGGACGCCAGGCCCTACAGCATCCTGGAAGCCACGA
AAGGGCTGTGCCCCGCCTGCGGCAACCCCATCATGAAGGAAGAAGGGAAAGTAGCCTGGCGCTGCACCAACTTCACCTGC
CCCGCGCAGGCCGTCACGGGCATCACCCATTTCTGTTCCCGGTCCGCTCTGGATGTGGAAAGCATCGGCTCTTCCGTGGC
GGAAGCGCTGAGAAGCTCCGGACTGGCGTCCTCCGCGCTGGACCTTTTCTCCCTGATGCCGGACCAGCTCGCCAACCTGA
ACCTGGGCACGCCGGAAGAACCGCGCCGTTACGGGGAAAAGAATGCCCGGAAAGCGCTGGACGCCCTGCAAAACGCGCGA
GGGCTTCCTCTGGAGCGCTGGCTCATCGCCTTCGGCATTCCCCTGGTGGGGGAAGTTGTCGCCAAGGCGCTGGCGGACAC
GCATCCCGACCTGAAGCATGTGGCGGATTCTCCCTACCTCCGGGACATCGTCCGCCTGGATGAACTGGTGGAACAGGCCG
CCAAAACCAACCCCAACACCCGCGAAAACAAAAAGGCGGTGAAGGAAGGCGCGCTTTCCGCAGAGGCGGTACAGGAGCGC
CACCAGGAACTGATGGAGGAAATCGACCGCCTGACCGCTCCCTATCTGGCAACGGGCTACCTGCGTAAAAACACGGCCAA
ATTCAGCTATGGCTCCGAAATCGGCGTGGCGGCGGCCAAATCCCTCCGGAGCTTTTTCGCCTCCGCAGCAGGGAACCATA
CCATGGACGTCCTGCACAGACTGGGCATCAATCCCCAATCCCAATCTTACCGGGCCAGCCTTCTCGAAATCCCGGCGGGC
TCCCTGTCCGGCAAAACCTTCGTCATCACAGGAACGCTCAGCCATCCGCGGGATTACTTTGAACAGCTTATCGCCTCCCA
CGGGGGAAAAGCCACCGGAGCCATCTCCAAATCCACCTCCTGCCTCCTGGCAGGCAACGGAGGAGGCTCCAAGCGGGACA
AGGCCCTCAAACTGGGCGTGCCCGTCATTTCCGAGGAAGACTTCTACAAAATGATCGGGAATTAA

Upstream 100 bases:

>100_bases
CGTCAACGGAACGGTCCTGTTCAAGGCTCATGAACATTCCAAAACTCCCTGTTCCGTCATCAAACTGAGCAAATAGCCAT
TTTCCCCACTCATTTTTCCT

Downstream 100 bases:

>100_bases
TTGCCGCAAAACAGCGGAAACGGGAAGCAGGAACTGAAACGCACGCATCATGCGCCCGGCTGCCCGTATCTTCGTATTCC
CTTCGCTTTTTTCCGTGCGC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 794; Mature: 794

Protein sequence:

>794_residues
MEEDLFSLAAGKQPSQQATNETAPRAGEARENAGTDHPGNAEDPAHRRIDYLRSELRRHNRLYYEQAEPEISDAEYDALF
LELEKLEKAHPELSDPDSPTRRVGGAPLQGFNQIRHAVPMLSIDDIFEQRDAPVPDEELAEFYHKLSRALQTENVPVSVE
PKIDGVALSIMYRNGKLAYAATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRGEVFMPNEAFAKLNEERDADGLP
AFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAYEGPELRDAKDFWNMLRHCGIPCNEPVYYTDSLESTRQAVRDIDR
LRHTLPYGTDGAVIKISSTATREALGATARAPRWAAAYKFPPEQKETTLLNIVVQVGRTGVLTPVAELQPVLLSGSTVAR
ATLHNQDEIDRKDVRIGDTVLVEKAGEIIPAVLKVNLSKRPQDARPYSILEATKGLCPACGNPIMKEEGKVAWRCTNFTC
PAQAVTGITHFCSRSALDVESIGSSVAEALRSSGLASSALDLFSLMPDQLANLNLGTPEEPRRYGEKNARKALDALQNAR
GLPLERWLIAFGIPLVGEVVAKALADTHPDLKHVADSPYLRDIVRLDELVEQAAKTNPNTRENKKAVKEGALSAEAVQER
HQELMEEIDRLTAPYLATGYLRKNTAKFSYGSEIGVAAAKSLRSFFASAAGNHTMDVLHRLGINPQSQSYRASLLEIPAG
SLSGKTFVITGTLSHPRDYFEQLIASHGGKATGAISKSTSCLLAGNGGGSKRDKALKLGVPVISEEDFYKMIGN

Sequences:

>Translated_794_residues
MEEDLFSLAAGKQPSQQATNETAPRAGEARENAGTDHPGNAEDPAHRRIDYLRSELRRHNRLYYEQAEPEISDAEYDALF
LELEKLEKAHPELSDPDSPTRRVGGAPLQGFNQIRHAVPMLSIDDIFEQRDAPVPDEELAEFYHKLSRALQTENVPVSVE
PKIDGVALSIMYRNGKLAYAATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRGEVFMPNEAFAKLNEERDADGLP
AFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAYEGPELRDAKDFWNMLRHCGIPCNEPVYYTDSLESTRQAVRDIDR
LRHTLPYGTDGAVIKISSTATREALGATARAPRWAAAYKFPPEQKETTLLNIVVQVGRTGVLTPVAELQPVLLSGSTVAR
ATLHNQDEIDRKDVRIGDTVLVEKAGEIIPAVLKVNLSKRPQDARPYSILEATKGLCPACGNPIMKEEGKVAWRCTNFTC
PAQAVTGITHFCSRSALDVESIGSSVAEALRSSGLASSALDLFSLMPDQLANLNLGTPEEPRRYGEKNARKALDALQNAR
GLPLERWLIAFGIPLVGEVVAKALADTHPDLKHVADSPYLRDIVRLDELVEQAAKTNPNTRENKKAVKEGALSAEAVQER
HQELMEEIDRLTAPYLATGYLRKNTAKFSYGSEIGVAAAKSLRSFFASAAGNHTMDVLHRLGINPQSQSYRASLLEIPAG
SLSGKTFVITGTLSHPRDYFEQLIASHGGKATGAISKSTSCLLAGNGGGSKRDKALKLGVPVISEEDFYKMIGN
>Mature_794_residues
MEEDLFSLAAGKQPSQQATNETAPRAGEARENAGTDHPGNAEDPAHRRIDYLRSELRRHNRLYYEQAEPEISDAEYDALF
LELEKLEKAHPELSDPDSPTRRVGGAPLQGFNQIRHAVPMLSIDDIFEQRDAPVPDEELAEFYHKLSRALQTENVPVSVE
PKIDGVALSIMYRNGKLAYAATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRGEVFMPNEAFAKLNEERDADGLP
AFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAYEGPELRDAKDFWNMLRHCGIPCNEPVYYTDSLESTRQAVRDIDR
LRHTLPYGTDGAVIKISSTATREALGATARAPRWAAAYKFPPEQKETTLLNIVVQVGRTGVLTPVAELQPVLLSGSTVAR
ATLHNQDEIDRKDVRIGDTVLVEKAGEIIPAVLKVNLSKRPQDARPYSILEATKGLCPACGNPIMKEEGKVAWRCTNFTC
PAQAVTGITHFCSRSALDVESIGSSVAEALRSSGLASSALDLFSLMPDQLANLNLGTPEEPRRYGEKNARKALDALQNAR
GLPLERWLIAFGIPLVGEVVAKALADTHPDLKHVADSPYLRDIVRLDELVEQAAKTNPNTRENKKAVKEGALSAEAVQER
HQELMEEIDRLTAPYLATGYLRKNTAKFSYGSEIGVAAAKSLRSFFASAAGNHTMDVLHRLGINPQSQSYRASLLEIPAG
SLSGKTFVITGTLSHPRDYFEQLIASHGGKATGAISKSTSCLLAGNGGGSKRDKALKLGVPVISEEDFYKMIGN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=544, Percent_Identity=44.4852941176471, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI87082305, Length=538, Percent_Identity=25.092936802974, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_AKKM8 (B2UL69)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001876639.1
- ProteinModelPortal:   B2UL69
- GeneID:   6275234
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0011
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 86425; Mature: 86425

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 162-162 BINDING 160-160 BINDING 183-183 BINDING 219-219 BINDING 335-335 BINDING 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDLFSLAAGKQPSQQATNETAPRAGEARENAGTDHPGNAEDPAHRRIDYLRSELRRHN
CCCHHHHHHCCCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RLYYEQAEPEISDAEYDALFLELEKLEKAHPELSDPDSPTRRVGGAPLQGFNQIRHAVPM
CEEHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCC
LSIDDIFEQRDAPVPDEELAEFYHKLSRALQTENVPVSVEPKIDGVALSIMYRNGKLAYA
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCEEEEE
ATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRGEVFMPNEAFAKLNEERDADGLP
EECCCCCCCCCCCCCHHHHHCCCEECCCCCCCCEEECCCEECCCHHHHHHCHHCCCCCCC
AFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAYEGPELRDAKDFWNMLRHCGIPCNE
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PVYYTDSLESTRQAVRDIDRLRHTLPYGTDGAVIKISSTATREALGATARAPRWAAAYKF
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCCCCCCCCCEECCC
PPEQKETTLLNIVVQVGRTGVLTPVAELQPVLLSGSTVARATLHNQDEIDRKDVRIGDTV
CCCCHHHHHHHHHHHHCCCCCCCCHHHHCHHHCCCCHHHHHHHCCCCCCCHHHCCCCCHH
LVEKAGEIIPAVLKVNLSKRPQDARPYSILEATKGLCPACGNPIMKEEGKVAWRCTNFTC
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCEEEEECCCCC
PAQAVTGITHFCSRSALDVESIGSSVAEALRSSGLASSALDLFSLMPDQLANLNLGTPEE
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCCCCCCHH
PRRYGEKNARKALDALQNARGLPLERWLIAFGIPLVGEVVAKALADTHPDLKHVADSPYL
HHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCHHH
RDIVRLDELVEQAAKTNPNTRENKKAVKEGALSAEAVQERHQELMEEIDRLTAPYLATGY
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
LRKNTAKFSYGSEIGVAAAKSLRSFFASAAGNHTMDVLHRLGINPQSQSYRASLLEIPAG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SLSGKTFVITGTLSHPRDYFEQLIASHGGKATGAISKSTSCLLAGNGGGSKRDKALKLGV
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECC
PVISEEDFYKMIGN
CCCCCCHHHHHHCC
>Mature Secondary Structure
MEEDLFSLAAGKQPSQQATNETAPRAGEARENAGTDHPGNAEDPAHRRIDYLRSELRRHN
CCCHHHHHHCCCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RLYYEQAEPEISDAEYDALFLELEKLEKAHPELSDPDSPTRRVGGAPLQGFNQIRHAVPM
CEEHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCC
LSIDDIFEQRDAPVPDEELAEFYHKLSRALQTENVPVSVEPKIDGVALSIMYRNGKLAYA
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCEEEEE
ATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRGEVFMPNEAFAKLNEERDADGLP
EECCCCCCCCCCCCCHHHHHCCCEECCCCCCCCEEECCCEECCCHHHHHHCHHCCCCCCC
AFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAYEGPELRDAKDFWNMLRHCGIPCNE
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PVYYTDSLESTRQAVRDIDRLRHTLPYGTDGAVIKISSTATREALGATARAPRWAAAYKF
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCCCCCCCCCEECCC
PPEQKETTLLNIVVQVGRTGVLTPVAELQPVLLSGSTVARATLHNQDEIDRKDVRIGDTV
CCCCHHHHHHHHHHHHCCCCCCCCHHHHCHHHCCCCHHHHHHHCCCCCCCHHHCCCCCHH
LVEKAGEIIPAVLKVNLSKRPQDARPYSILEATKGLCPACGNPIMKEEGKVAWRCTNFTC
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCEEEEECCCCC
PAQAVTGITHFCSRSALDVESIGSSVAEALRSSGLASSALDLFSLMPDQLANLNLGTPEE
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHCCCCCCCHH
PRRYGEKNARKALDALQNARGLPLERWLIAFGIPLVGEVVAKALADTHPDLKHVADSPYL
HHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCHHH
RDIVRLDELVEQAAKTNPNTRENKKAVKEGALSAEAVQERHQELMEEIDRLTAPYLATGY
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
LRKNTAKFSYGSEIGVAAAKSLRSFFASAAGNHTMDVLHRLGINPQSQSYRASLLEIPAG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SLSGKTFVITGTLSHPRDYFEQLIASHGGKATGAISKSTSCLLAGNGGGSKRDKALKLGV
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECC
PVISEEDFYKMIGN
CCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA