Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 186477826

GI number: 186477826

Start: 3460615

End: 3462225

Strand: Reverse

Name: oprM [H]

Synonym: Bphy_3082

Alternate gene names: 186477826

Gene position: 3462225-3460615 (Counterclockwise)

Preceding gene: 186477827

Following gene: 186477817

Centisome position: 99.51

GC content: 67.91

Gene sequence:

>1611_bases
ATGCAGTTTGATCGAACTTCGCGCGCGCGCGCCGCTGTCGCGAACGTCGCGGGTCCACGCGTCCTGAGCATCGCCGCTTG
CGCCGCGCTCGCCTTCGCACTTTCCGGCTGCGCCGTCGGCCCCGACTACAAGCGCCCGTCCGTCGACATTCCGGCTTCGT
ACAAAGAAGCCGCCGACGGCTGGAAAGTCGCACAGCCCGCCGACCAGCACGACCGCGGCGCTTGGTGGTCGATCTACAAC
GATGCGCAGTTGAACGCGCTCGAAGAGAAGCTCAACGCATCGAACCAGAGCATCGCGCAGTTCGCCGCCGCGTATCGGCA
GGCGCGCGCGCTGGTCGGCGAAGCGCGCGCGGCGTATTTCCCGGTGATCAGTGCATCGGCGGACGCGTCCCGCTCGCGCA
CGCCGAGCCGCAGCTTTAGTGGCTCCGTGACAGGCGGAGGCGGCGGAACCACGTCGTCGCTGAGCAGTTCGGGCTCGATC
AGCAACAGCTACAGCCTGTCGCTCGACGCCACCTGGGAACCCGACCTGTGGGGCAAGGTGAGCCGCACCGTCGCCGGTCA
GCAGGCTGGCCAGCAGTCGGCCGCCGCCGATCTGGCGAACGCGCGCCTGTCCGCTCAAGCGACGCTCGCGCAAACCTACT
TCAACACCCGTTCGCTTGACGCCCAGCAAAAGCTGCTCGACGACACCGTCGCCGCATACCAGCGCTCGCTCACCCTCACG
CAGAACCGCTATGCGCAGGGCGTCGCGGCGCGCTCCGACGTGATCCAGGCGCAAACCCAGCTGCAGTCGGCGCAGGCCGC
CGCCATCGATAACGGGGTCGCGCGCGCGCAGAACGAACATGCGATCGCCGTGCTCGTCGGCCAGCCGGCGTCTACCTTCT
CGCTGCCGCCCGCGCCGCTCGATGCCGTGCCGCCCGTCGTGCCCGCACAACTGCCGTCCGCGCTGCTCGAACGGCGCCCC
GACATCGCGTCTGCCGAACGCAAGGCTGCCGCGGCGAACGAGCAGATCGGCATCGCGATTGCCGCGTTCTTCCCCACGCT
CACGCTGTCTGCGAGCGGCGGCTTCGAAAGCTCGGTGTTCTCGCAATTGCTGCAGATGCCGTCGCGTTTCTGGACGCTAG
GGCCGCAACTCGCGGGCACGATCTTCGATGCAGGTTTGCGCAAGGCGCAGACGGACGCCGCGCGTGCCACCTACGACCAG
GACGTCGCCACCTATCGGCAAACGGTGCTGACAGCTTTCCAGGACGTGGAAGACAACCTGTCGTCGCTGCGCATTCTCGA
GCAGGAAATCACGGTGCAACAACAGGCCGTCCAGTCTGCGCAGCAGGCGCTCGATATCGTCACGAACCAGTACAAGTCCG
GCACCGTGGATTACCTGAACGTGCTGACCGCGCAGACCACGGCATTCACCGCGCAGCAGAAGCTCGCGAGCATCGCGGGT
CAGCGGATGGTGTCGTCGGTGGGGCTCGTCAAGGCGCTCGGCGGCGGCTGGGATGCGTCGCAGATGAACCGCGAGACGGG
CGATATCGCGGCGCCCGCGCCGGCTTCCGAACCCGCGGCGCCCGTCACACCCGCCACGCCCGTCACGAAAGCATCCGCCG
CGGCACGCTGA

Upstream 100 bases:

>100_bases
CCATTCGAGCCAACGCGACCACGCACCGGACATTGCGTGCGCGGCCGCCTTGCAAATCTCCGCACATGACGCCATCCGAA
TCGACAGGGAATTCATTTGC

Downstream 100 bases:

>100_bases
TCGCAGTCGTTATCCGCGCGACGCAAAACGGCCGCCATGCGCAAACATGGCGGCCGTCGTGTTTTGGAGCGGCCCGGAAA
AGTCAGTGCGTGAACGTGAG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MQFDRTSRARAAVANVAGPRVLSIAACAALAFALSGCAVGPDYKRPSVDIPASYKEAADGWKVAQPADQHDRGAWWSIYN
DAQLNALEEKLNASNQSIAQFAAAYRQARALVGEARAAYFPVISASADASRSRTPSRSFSGSVTGGGGGTTSSLSSSGSI
SNSYSLSLDATWEPDLWGKVSRTVAGQQAGQQSAAADLANARLSAQATLAQTYFNTRSLDAQQKLLDDTVAAYQRSLTLT
QNRYAQGVAARSDVIQAQTQLQSAQAAAIDNGVARAQNEHAIAVLVGQPASTFSLPPAPLDAVPPVVPAQLPSALLERRP
DIASAERKAAAANEQIGIAIAAFFPTLTLSASGGFESSVFSQLLQMPSRFWTLGPQLAGTIFDAGLRKAQTDAARATYDQ
DVATYRQTVLTAFQDVEDNLSSLRILEQEITVQQQAVQSAQQALDIVTNQYKSGTVDYLNVLTAQTTAFTAQQKLASIAG
QRMVSSVGLVKALGGGWDASQMNRETGDIAAPAPASEPAAPVTPATPVTKASAAAR

Sequences:

>Translated_536_residues
MQFDRTSRARAAVANVAGPRVLSIAACAALAFALSGCAVGPDYKRPSVDIPASYKEAADGWKVAQPADQHDRGAWWSIYN
DAQLNALEEKLNASNQSIAQFAAAYRQARALVGEARAAYFPVISASADASRSRTPSRSFSGSVTGGGGGTTSSLSSSGSI
SNSYSLSLDATWEPDLWGKVSRTVAGQQAGQQSAAADLANARLSAQATLAQTYFNTRSLDAQQKLLDDTVAAYQRSLTLT
QNRYAQGVAARSDVIQAQTQLQSAQAAAIDNGVARAQNEHAIAVLVGQPASTFSLPPAPLDAVPPVVPAQLPSALLERRP
DIASAERKAAAANEQIGIAIAAFFPTLTLSASGGFESSVFSQLLQMPSRFWTLGPQLAGTIFDAGLRKAQTDAARATYDQ
DVATYRQTVLTAFQDVEDNLSSLRILEQEITVQQQAVQSAQQALDIVTNQYKSGTVDYLNVLTAQTTAFTAQQKLASIAG
QRMVSSVGLVKALGGGWDASQMNRETGDIAAPAPASEPAAPVTPATPVTKASAAAR
>Mature_536_residues
MQFDRTSRARAAVANVAGPRVLSIAACAALAFALSGCAVGPDYKRPSVDIPASYKEAADGWKVAQPADQHDRGAWWSIYN
DAQLNALEEKLNASNQSIAQFAAAYRQARALVGEARAAYFPVISASADASRSRTPSRSFSGSVTGGGGGTTSSLSSSGSI
SNSYSLSLDATWEPDLWGKVSRTVAGQQAGQQSAAADLANARLSAQATLAQTYFNTRSLDAQQKLLDDTVAAYQRSLTLT
QNRYAQGVAARSDVIQAQTQLQSAQAAAIDNGVARAQNEHAIAVLVGQPASTFSLPPAPLDAVPPVVPAQLPSALLERRP
DIASAERKAAAANEQIGIAIAAFFPTLTLSASGGFESSVFSQLLQMPSRFWTLGPQLAGTIFDAGLRKAQTDAARATYDQ
DVATYRQTVLTAFQDVEDNLSSLRILEQEITVQQQAVQSAQQALDIVTNQYKSGTVDYLNVLTAQTTAFTAQQKLASIAG
QRMVSSVGLVKALGGGWDASQMNRETGDIAAPAPASEPAAPVTPATPVTKASAAAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=29.746835443038, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI2367348, Length=476, Percent_Identity=25.2100840336134, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56099; Mature: 56099

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDRTSRARAAVANVAGPRVLSIAACAALAFALSGCAVGPDYKRPSVDIPASYKEAADG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
WKVAQPADQHDRGAWWSIYNDAQLNALEEKLNASNQSIAQFAAAYRQARALVGEARAAYF
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PVISASADASRSRTPSRSFSGSVTGGGGGTTSSLSSSGSISNSYSLSLDATWEPDLWGKV
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHH
SRTVAGQQAGQQSAAADLANARLSAQATLAQTYFNTRSLDAQQKLLDDTVAAYQRSLTLT
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
QNRYAQGVAARSDVIQAQTQLQSAQAAAIDNGVARAQNEHAIAVLVGQPASTFSLPPAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
DAVPPVVPAQLPSALLERRPDIASAERKAAAANEQIGIAIAAFFPTLTLSASGGFESSVF
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHEEHHHHHEEECCCCCHHHHH
SQLLQMPSRFWTLGPQLAGTIFDAGLRKAQTDAARATYDQDVATYRQTVLTAFQDVEDNL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLRILEQEITVQQQAVQSAQQALDIVTNQYKSGTVDYLNVLTAQTTAFTAQQKLASIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRMVSSVGLVKALGGGWDASQMNRETGDIAAPAPASEPAAPVTPATPVTKASAAAR
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFDRTSRARAAVANVAGPRVLSIAACAALAFALSGCAVGPDYKRPSVDIPASYKEAADG
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
WKVAQPADQHDRGAWWSIYNDAQLNALEEKLNASNQSIAQFAAAYRQARALVGEARAAYF
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PVISASADASRSRTPSRSFSGSVTGGGGGTTSSLSSSGSISNSYSLSLDATWEPDLWGKV
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHH
SRTVAGQQAGQQSAAADLANARLSAQATLAQTYFNTRSLDAQQKLLDDTVAAYQRSLTLT
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
QNRYAQGVAARSDVIQAQTQLQSAQAAAIDNGVARAQNEHAIAVLVGQPASTFSLPPAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
DAVPPVVPAQLPSALLERRPDIASAERKAAAANEQIGIAIAAFFPTLTLSASGGFESSVF
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHEEHHHHHEEECCCCCHHHHH
SQLLQMPSRFWTLGPQLAGTIFDAGLRKAQTDAARATYDQDVATYRQTVLTAFQDVEDNL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLRILEQEITVQQQAVQSAQQALDIVTNQYKSGTVDYLNVLTAQTTAFTAQQKLASIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRMVSSVGLVKALGGGWDASQMNRETGDIAAPAPASEPAAPVTPATPVTKASAAAR
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]