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The map label for this gene is priA [H]

Identifier: 186477768

GI number: 186477768

Start: 3393280

End: 3395538

Strand: Reverse

Name: priA [H]

Synonym: Bphy_3022

Alternate gene names: 186477768

Gene position: 3395538-3393280 (Counterclockwise)

Preceding gene: 186477769

Following gene: 186477767

Centisome position: 97.6

GC content: 67.33

Gene sequence:

>2259_bases
ATGCGGCTCGAAGCGCTGACCGACACCTTCGTCCGCGTTGCGCTCGATCATCCGTTGCCGACGCTTTTCGACTACCGCTG
CGACGGCGCCTGGGCGCCGGCGCCGGGCATGCTCGTCAGCGTGCCATTCGGCAAGCGGCATATCGTGGGGCTCGTCATGG
AAGTGACCGCTTACAGCGACGTGCCTGCCGATCGCATCAAATCGGTCCACAGCGTCTGCACCGCGTGCCCGCCGCTGTCG
GAGCATTGGCTGCAACTCGCGCAATTCGCCGCCGACTATTACCAGCGCGGCATCGGTGAAGTCGCGCTGCCCGCGCTTCC
GCAGGCGCTGCGTGATGCGCCGCGCTGGACGCGCCTGCTCGCGCCCGAGGAACGCTATCGCCTGCTGCCTGCGGGCCGCG
CGGCGTTGCCCGGCGCGTTGCCTGCCCGCGCTACCGCGCTGCGCCGTCTTGCAGCCGCGCTGGCTGAAGCGGAGTCGCTG
CCAGCCGTCGATGCGCGCGCGCTGCATCCCAAGGCCACCGCGACGCTGGAAGCGTGGCAAGCCGAAGGCTGGGTCGCGCT
TGACGTGCTCGATCCCGCAGCGCCACGTCCAGCGCAGGATCACACGCCGCAAAGCGTGTCGCTGCCGACACTCACGGACG
AACAGACGACCGCGGTCGAAGCGATCCGCGACGTGCAAGGCTTCGCGCCGTTTCTGCTGCACGGCGTAACGGGCAGCGGC
AAGACGGAGGTGTATCTGCGCGCGTTGGCTGGCTTGCTGGCCGCTAATCCCAACGCGCAAGCGCTCGTACTCGTGCCCGA
GATCAACCTGACGCCGCAGTTCGAAGCGGCGTTTCGAGCACGTTTCGCGTCGATGGACGATGCCGAAATCGTCACGCTGC
ATAGCGGTCTGGCCGAAGGCGAACGCGCCCGCAACTGGTTCGCCGCGCACACGGGACGCGCGCGCATCGTGCTGGGTACG
CGGCTCGCGGTGCTGGCGTCGTTGCCGCATCTCGCCATGATCGTCGTCGACGAGGAGCACGATCCCGCTTACAAGCAGCA
GGAAGGTCTGCGCTATTCCGCGCGCGATCTGGCGATCTGGCGGGCGAAACAGCTCGCCATTCCCGTCGTTTTGGGATCGG
CGACGCCTTCGCTCGAAAGCTGGTGGCAGGTCGATCAGGGACGCTACAGACGGCTCACGTTGTCGCGCCGCGCCGTTGCG
GATGCCGTGTTGCCCACCGTCCGGCTCGTCGATCTGGAAGATGAACGCAGGCGCGGGCGCGCGTCGGTGGAAGGTCTGTC
CGGGCCGCTGATCGCGGCGCTAAAAACGCGTCTCGAGCGTGGCGAACAAAGCCTCGTGTTCCTGAACCGTCGCGGCTACG
CGCCGCAGCTGGCGTGCGACGCATGCGGCTGGGTCGCGGGCTGCCCGCGATGCAGCGCCTATGCGGTGCTGCATAAGCCG
GAACGGGCGCTGCGTTGCCACCATTGCGGCTGGGAAGCACGCATTCCACGCTCGTGTCCGGAATGCGGCAATGTCGATAT
AGCGCCGCTCGGACGCGGCACGCAGCGCATCGAAGAGGCGCTCGCCAGCGCGGTTCCGGGTGCGCGCGTGCTGCGCATCG
ATGCCGACAGCACGCGCCGCAAGGGCAGCGCGCAGGCGCTGTTCTCCGACGTGCATGCGGGCGAAGTCGACATTCTGGTC
GGCACTCAGATGATCGCGAAGGGGCACGATTTCCGTCGCGTGTCGCTGGTCGGCGTGCTGAACGCGGACACGGCGCTCTT
TTCCCACGATTTCCGCGCCAGCGAGCGCCTGTTCGCGCAACTGATGCAGGTAAGCGGCCGCGCAGGCCGCGCCGGATTAC
CGGGTGAAGTGCTGGTGCAAACGCGTTACCCGACACACGCGCTGTATCACGCGTTGGCTCGCCAGGATTACGTTGGCTTT
GCCAATTCGACCCTTTCCGAACGGCGCGACGCGCATCTGCCGCCCTTCGTCTATCAGGCGATGCTTCGCGCCGAAGGACG
CACGCTCGACGCCGCGCTCACATTCCTGCAACAGGCGGCAACGGCGTTGACCGACATCCCCGCTGCGAGCCGCGTGACCG
TGTACGACGCGGTACCGCTCACCATCGTCAAGGTGATGCACGTGCATCGCGCGCAATTGCTGGTGGAGAGCGCGTCACGC
GCTGCGCTGCAGGCGACGCTGCGTGCGTGGCAGCCGCTGCTGCGCACGCTGAAAGGCGTGCTGCGCTGGAGCATCGAAGT
CGATCCGCTGGACATCTGA

Upstream 100 bases:

>100_bases
CGATGCAAAATCAAAACTTAGCGCTGAATTTGAAGTTTTACTTTAAGTGTGGCAGCCGCAGCATCGGTCCTGTGAATAAC
TCTTTCCGCCGACATCGTTC

Downstream 100 bases:

>100_bases
CTCGCGCTCCAACAAGTGGCGATCGCGCGCCGTCTCCGCGCAAATCCATTTAAGTCATATCGATCAAACGAAAGGTCGTT
TCGTTTGGCGAGATCGCTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MRLEALTDTFVRVALDHPLPTLFDYRCDGAWAPAPGMLVSVPFGKRHIVGLVMEVTAYSDVPADRIKSVHSVCTACPPLS
EHWLQLAQFAADYYQRGIGEVALPALPQALRDAPRWTRLLAPEERYRLLPAGRAALPGALPARATALRRLAAALAEAESL
PAVDARALHPKATATLEAWQAEGWVALDVLDPAAPRPAQDHTPQSVSLPTLTDEQTTAVEAIRDVQGFAPFLLHGVTGSG
KTEVYLRALAGLLAANPNAQALVLVPEINLTPQFEAAFRARFASMDDAEIVTLHSGLAEGERARNWFAAHTGRARIVLGT
RLAVLASLPHLAMIVVDEEHDPAYKQQEGLRYSARDLAIWRAKQLAIPVVLGSATPSLESWWQVDQGRYRRLTLSRRAVA
DAVLPTVRLVDLEDERRRGRASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYAVLHKP
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILV
GTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGF
ANSTLSERRDAHLPPFVYQAMLRAEGRTLDAALTFLQQAATALTDIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
AALQATLRAWQPLLRTLKGVLRWSIEVDPLDI

Sequences:

>Translated_752_residues
MRLEALTDTFVRVALDHPLPTLFDYRCDGAWAPAPGMLVSVPFGKRHIVGLVMEVTAYSDVPADRIKSVHSVCTACPPLS
EHWLQLAQFAADYYQRGIGEVALPALPQALRDAPRWTRLLAPEERYRLLPAGRAALPGALPARATALRRLAAALAEAESL
PAVDARALHPKATATLEAWQAEGWVALDVLDPAAPRPAQDHTPQSVSLPTLTDEQTTAVEAIRDVQGFAPFLLHGVTGSG
KTEVYLRALAGLLAANPNAQALVLVPEINLTPQFEAAFRARFASMDDAEIVTLHSGLAEGERARNWFAAHTGRARIVLGT
RLAVLASLPHLAMIVVDEEHDPAYKQQEGLRYSARDLAIWRAKQLAIPVVLGSATPSLESWWQVDQGRYRRLTLSRRAVA
DAVLPTVRLVDLEDERRRGRASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYAVLHKP
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILV
GTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGF
ANSTLSERRDAHLPPFVYQAMLRAEGRTLDAALTFLQQAATALTDIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
AALQATLRAWQPLLRTLKGVLRWSIEVDPLDI
>Mature_752_residues
MRLEALTDTFVRVALDHPLPTLFDYRCDGAWAPAPGMLVSVPFGKRHIVGLVMEVTAYSDVPADRIKSVHSVCTACPPLS
EHWLQLAQFAADYYQRGIGEVALPALPQALRDAPRWTRLLAPEERYRLLPAGRAALPGALPARATALRRLAAALAEAESL
PAVDARALHPKATATLEAWQAEGWVALDVLDPAAPRPAQDHTPQSVSLPTLTDEQTTAVEAIRDVQGFAPFLLHGVTGSG
KTEVYLRALAGLLAANPNAQALVLVPEINLTPQFEAAFRARFASMDDAEIVTLHSGLAEGERARNWFAAHTGRARIVLGT
RLAVLASLPHLAMIVVDEEHDPAYKQQEGLRYSARDLAIWRAKQLAIPVVLGSATPSLESWWQVDQGRYRRLTLSRRAVA
DAVLPTVRLVDLEDERRRGRASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYAVLHKP
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILV
GTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGF
ANSTLSERRDAHLPPFVYQAMLRAEGRTLDAALTFLQQAATALTDIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
AALQATLRAWQPLLRTLKGVLRWSIEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=757, Percent_Identity=41.2153236459709, Blast_Score=487, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82043; Mature: 82043

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEALTDTFVRVALDHPLPTLFDYRCDGAWAPAPGMLVSVPFGKRHIVGLVMEVTAYSD
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
VPADRIKSVHSVCTACPPLSEHWLQLAQFAADYYQRGIGEVALPALPQALRDAPRWTRLL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHC
APEERYRLLPAGRAALPGALPARATALRRLAAALAEAESLPAVDARALHPKATATLEAWQ
CCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHC
AEGWVALDVLDPAAPRPAQDHTPQSVSLPTLTDEQTTAVEAIRDVQGFAPFLLHGVTGSG
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCC
KTEVYLRALAGLLAANPNAQALVLVPEINLTPQFEAAFRARFASMDDAEIVTLHSGLAEG
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCC
ERARNWFAAHTGRARIVLGTRLAVLASLPHLAMIVVDEEHDPAYKQQEGLRYSARDLAIW
HHHHHHHHCCCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHH
RAKQLAIPVVLGSATPSLESWWQVDQGRYRRLTLSRRAVADAVLPTVRLVDLEDERRRGR
HHHHEEEEEEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHCCEEEECCCCHHHCCC
ASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYAVLHKP
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECC
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALASAVPGARVLRIDADSTRR
HHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
KGSAQALFSDVHAGEVDILVGTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQ
CCHHHHHHHHCCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHH
LMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGFANSTLSERRDAHLPPFVYQA
HHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHCHHHHHCCCCCCHHHHHH
MLRAEGRTLDAALTFLQQAATALTDIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AALQATLRAWQPLLRTLKGVLRWSIEVDPLDI
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure
MRLEALTDTFVRVALDHPLPTLFDYRCDGAWAPAPGMLVSVPFGKRHIVGLVMEVTAYSD
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
VPADRIKSVHSVCTACPPLSEHWLQLAQFAADYYQRGIGEVALPALPQALRDAPRWTRLL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHC
APEERYRLLPAGRAALPGALPARATALRRLAAALAEAESLPAVDARALHPKATATLEAWQ
CCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHC
AEGWVALDVLDPAAPRPAQDHTPQSVSLPTLTDEQTTAVEAIRDVQGFAPFLLHGVTGSG
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCC
KTEVYLRALAGLLAANPNAQALVLVPEINLTPQFEAAFRARFASMDDAEIVTLHSGLAEG
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCC
ERARNWFAAHTGRARIVLGTRLAVLASLPHLAMIVVDEEHDPAYKQQEGLRYSARDLAIW
HHHHHHHHCCCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHH
RAKQLAIPVVLGSATPSLESWWQVDQGRYRRLTLSRRAVADAVLPTVRLVDLEDERRRGR
HHHHEEEEEEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHCCEEEECCCCHHHCCC
ASVEGLSGPLIAALKTRLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYAVLHKP
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECC
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALASAVPGARVLRIDADSTRR
HHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
KGSAQALFSDVHAGEVDILVGTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQ
CCHHHHHHHHCCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHH
LMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGFANSTLSERRDAHLPPFVYQA
HHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHCHHHHHCCCCCCHHHHHH
MLRAEGRTLDAALTFLQQAATALTDIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
AALQATLRAWQPLLRTLKGVLRWSIEVDPLDI
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]