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The map label for this gene is cca

Identifier: 186477645

GI number: 186477645

Start: 3251453

End: 3252706

Strand: Reverse

Name: cca

Synonym: Bphy_2897

Alternate gene names: 186477645

Gene position: 3252706-3251453 (Counterclockwise)

Preceding gene: 186477646

Following gene: 186477642

Centisome position: 93.49

GC content: 65.55

Gene sequence:

>1254_bases
ATGAATATCTACGCAGTAGGCGGTGCGATCCGCGATGAACTGCTCGGCATGCCCGTGCAGGATCGCGATTACGTGGTGGT
CGGCGCGACGCCCGAGCAGATGATCGCGCAGGGCTTTCGCCCCGTCGGCAAGGACTTTCCGGTGTTCCTGCATCCCGACA
CGCAGGAGGAATACGCGCTCGCGCGTACGGAGCGCAAGACATCGGCGGGTTATCACGGCTTTCAGTTTTTCTACGCACCC
GATGTCACGCTGGAAGACGACCTCGCGCGACGCGACCTGACGATCAATGCGATGGCGCGCGAGGTTACGCCTGACGGCGC
GCTCATCGGCCCGGTGATCGATCCGTTCAACGGCCGGGAAGACCTCGAGCACCGCGTGTTCCGTCATGTCAGCGACGCGT
TTCTCGAAGATCCCGTGCGCATTCTGCGCGTCGCGCGCTTTTCGGCGCGCTTTGCGGATTTCAGCGTCGCACCCGAGACG
CTCGCGCTGATGCGCAAGATGGTCGAGGCGGGCGAAGTCGATGCACTCGTGCCGGAGCGCGTGTGGCAGGAAGTGTCGCG
CGGCTTGATGGAGAAGAAGCCGTCGCGCATGTTCGAGGTGCTGCGCGACTGCGGCGCACTCGCGCGCGTTCTGCCGGAGA
TCGACGCGCTCTATGGCGTGCCCCAACGCGCCGACTATCACCCGGAAGTGGATACGGGTGTGCATGTGATGATGGTCGTG
GATCACGCGGCCGCGCAGAACTATGCGCTGGCCGTTCGCTTCGCCGCGCTCACACACGATCTGGGCAAGGCGACCACGCC
CGAAGACGTGTTGCCGCGTCATATCGGCCACGAAGGGCGCAGCGTCGATCTGCTCAAGCCGCTGTGCGAGCGTTTGCGCG
TGCCGAACGAATGCCGCGATCTCGCGCTGCTGGTGGCGCGCGAGCACGGCAACATCCATCGCGTGATGGAAACGAAGGCG
GCCGGTCTCGTGCGGCTTTTCGAGCGCAGCGACGCGCTGCGCAAGCCGGCGCGTTTTGCCGAAGCATTGCAGGCCTGCGA
GGCCGACGCGCGCGGCCGGTTAGGCTTCGAAGCGAACGCGTATCCGCAGGCCGAGCGTTTGCGTCAGGCGCTCGTCGCGG
CGCGCGCCGTCGATGCCGGCGCGGTAGCGAAGTCGTATGAAAACAGTCCCGCGGAGATCAAAGACGCCGTGCATCGCGAG
CGCGTGCGGGCCGTGGCAAAAGCGCTGAACGAAGCGCCTTCCGGGCAAGCGTAG

Upstream 100 bases:

>100_bases
GTGCTTACGCGCGCCGCGTAACGGAACAACCGGCCGTCGCCGCGTGGATTAGCGATGCCTTGCGCGAAGGCCATGACATC
GCCAAGTACGACGTTTGCGC

Downstream 100 bases:

>100_bases
TTAAAGCAGCCGGGCGATCAGCGACAGCAGCATCGACAGCACCAGCGTCGACATGAAGGGAAACGGATATTCCTTTCCGA
ACAGCCGCAGCGTGACGTCG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNIYAVGGAIRDELLGMPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTQEEYALARTERKTSAGYHGFQFFYAP
DVTLEDDLARRDLTINAMAREVTPDGALIGPVIDPFNGREDLEHRVFRHVSDAFLEDPVRILRVARFSARFADFSVAPET
LALMRKMVEAGEVDALVPERVWQEVSRGLMEKKPSRMFEVLRDCGALARVLPEIDALYGVPQRADYHPEVDTGVHVMMVV
DHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALLVAREHGNIHRVMETKA
AGLVRLFERSDALRKPARFAEALQACEADARGRLGFEANAYPQAERLRQALVAARAVDAGAVAKSYENSPAEIKDAVHRE
RVRAVAKALNEAPSGQA

Sequences:

>Translated_417_residues
MNIYAVGGAIRDELLGMPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTQEEYALARTERKTSAGYHGFQFFYAP
DVTLEDDLARRDLTINAMAREVTPDGALIGPVIDPFNGREDLEHRVFRHVSDAFLEDPVRILRVARFSARFADFSVAPET
LALMRKMVEAGEVDALVPERVWQEVSRGLMEKKPSRMFEVLRDCGALARVLPEIDALYGVPQRADYHPEVDTGVHVMMVV
DHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALLVAREHGNIHRVMETKA
AGLVRLFERSDALRKPARFAEALQACEADARGRLGFEANAYPQAERLRQALVAARAVDAGAVAKSYENSPAEIKDAVHRE
RVRAVAKALNEAPSGQA
>Mature_417_residues
MNIYAVGGAIRDELLGMPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTQEEYALARTERKTSAGYHGFQFFYAP
DVTLEDDLARRDLTINAMAREVTPDGALIGPVIDPFNGREDLEHRVFRHVSDAFLEDPVRILRVARFSARFADFSVAPET
LALMRKMVEAGEVDALVPERVWQEVSRGLMEKKPSRMFEVLRDCGALARVLPEIDALYGVPQRADYHPEVDTGVHVMMVV
DHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALLVAREHGNIHRVMETKA
AGLVRLFERSDALRKPARFAEALQACEADARGRLGFEANAYPQAERLRQALVAARAVDAGAVAKSYENSPAEIKDAVHRE
RVRAVAKALNEAPSGQA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=409, Percent_Identity=54.278728606357, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BURP8 (B2JIM3)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001859115.1
- ProteinModelPortal:   B2JIM3
- SMR:   B2JIM3
- GeneID:   6244380
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2897
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- ProtClustDB:   PRK10885
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46270; Mature: 46270

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 143-143 BINDING 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYAVGGAIRDELLGMPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
ARTERKTSAGYHGFQFFYAPDVTLEDDLARRDLTINAMAREVTPDGALIGPVIDPFNGRE
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCEECCCCCCCCCHH
DLEHRVFRHVSDAFLEDPVRILRVARFSARFADFSVAPETLALMRKMVEAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHH
VWQEVSRGLMEKKPSRMFEVLRDCGALARVLPEIDALYGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
DHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRD
EHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALLVAREHGNIHRVMETKAAGLVRLFERSDALRKPARFAEALQACEADARGRLGFEANA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRQALVAARAVDAGAVAKSYENSPAEIKDAVHRERVRAVAKALNEAPSGQA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIYAVGGAIRDELLGMPVQDRDYVVVGATPEQMIAQGFRPVGKDFPVFLHPDTQEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
ARTERKTSAGYHGFQFFYAPDVTLEDDLARRDLTINAMAREVTPDGALIGPVIDPFNGRE
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCEECCCCCCCCCHH
DLEHRVFRHVSDAFLEDPVRILRVARFSARFADFSVAPETLALMRKMVEAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHH
VWQEVSRGLMEKKPSRMFEVLRDCGALARVLPEIDALYGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
DHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRD
EHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALLVAREHGNIHRVMETKAAGLVRLFERSDALRKPARFAEALQACEADARGRLGFEANA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YPQAERLRQALVAARAVDAGAVAKSYENSPAEIKDAVHRERVRAVAKALNEAPSGQA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA