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The map label for this gene is dsbC [H]

Identifier: 186477454

GI number: 186477454

Start: 3059761

End: 3060486

Strand: Reverse

Name: dsbC [H]

Synonym: Bphy_2706

Alternate gene names: 186477454

Gene position: 3060486-3059761 (Counterclockwise)

Preceding gene: 186477455

Following gene: 186477453

Centisome position: 87.97

GC content: 62.53

Gene sequence:

>726_bases
ATGAAGAAATCTATCCGCATCGCGGCGCTGGCGCTGGCCGTTGCGACCCTCGGACTCGGCTGTTCGGCCCAGGCCGACCA
GACCACCGACAAACTCAAGTCGACGCTGCAAGCGCGTCTGAGCGAAGCAACGATCAAAAACGTGTCGAAGTCGCCGATCG
ACGGGCTGTACGAAGTGAACCTCGGCTCGCAGATCATCTACAGCGATGCGTCGGGCGACCATCTGATCCTCGGCGACATG
ATCGACGCAAAGACGCGCAAGAATCTGACGGAAGCGCGTCTGTCCGAAACCAATCGCATCGATTTCGCGAGCCTGCCGTT
CGCGAATGCGGTGAAAGTGGTGAAGGGCAACGGTAGCCGCAAGATCGCCGTGTTTTCCGATCCGAACTGCCCGTACTGCA
AGCAGCTCGAAACGACGCTGAAGTCGATGGACAACATCACGGTCTACACCTTCCTGTACCCGGTGCTGTCGCCGGATTCG
ACGGTGAAGTCGAAGTCGATCTGGTGTTCCGCCGACCGTGCGAAGGCGTGGGAATCGTGGATGCAGGATCACCGCGCGCC
GACCGCGCCGGGCACCTGCGACACGGCCGCGATCGACAGGAATCTCGCGCTCGGCCAGTCGATGAACGTGAGCGGGACGC
CGACCGTTTTCCTCGCGGACGGCCGCCGCCTGCCCGGCGCGGTTCCCGCCGACGAACTGGACAAGGCGTTGTCGTCCGTG
CGCTGA

Upstream 100 bases:

>100_bases
GGCACGCAAGGCATACCATCTGCGCCAGGCGCGCGGTACTTGAACACAGGCGCTAACGAGCGGCATACGGCCGCGCGCCA
ACCGAATCAGGAGCAGCAGT

Downstream 100 bases:

>100_bases
GTGGCGCGCATCGTCTGAACGTTGTTGAAAGGAGGCGCGAGGCGCCTCCTGTGTTTTGAGCCTTCCGAACTTCCTCACGA
TTTTCCACACACGAGCGCCG

Product: putative thiol-disulfide interchange protein, DsbC

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKKSIRIAALALAVATLGLGCSAQADQTTDKLKSTLQARLSEATIKNVSKSPIDGLYEVNLGSQIIYSDASGDHLILGDM
IDAKTRKNLTEARLSETNRIDFASLPFANAVKVVKGNGSRKIAVFSDPNCPYCKQLETTLKSMDNITVYTFLYPVLSPDS
TVKSKSIWCSADRAKAWESWMQDHRAPTAPGTCDTAAIDRNLALGQSMNVSGTPTVFLADGRRLPGAVPADELDKALSSV
R

Sequences:

>Translated_241_residues
MKKSIRIAALALAVATLGLGCSAQADQTTDKLKSTLQARLSEATIKNVSKSPIDGLYEVNLGSQIIYSDASGDHLILGDM
IDAKTRKNLTEARLSETNRIDFASLPFANAVKVVKGNGSRKIAVFSDPNCPYCKQLETTLKSMDNITVYTFLYPVLSPDS
TVKSKSIWCSADRAKAWESWMQDHRAPTAPGTCDTAAIDRNLALGQSMNVSGTPTVFLADGRRLPGAVPADELDKALSSV
R
>Mature_241_residues
MKKSIRIAALALAVATLGLGCSAQADQTTDKLKSTLQARLSEATIKNVSKSPIDGLYEVNLGSQIIYSDASGDHLILGDM
IDAKTRKNLTEARLSETNRIDFASLPFANAVKVVKGNGSRKIAVFSDPNCPYCKQLETTLKSMDNITVYTFLYPVLSPDS
TVKSKSIWCSADRAKAWESWMQDHRAPTAPGTCDTAAIDRNLALGQSMNVSGTPTVFLADGRRLPGAVPADELDKALSSV
R

Specific function: Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789260, Length=245, Percent_Identity=29.7959183673469, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018950
- InterPro:   IPR009094
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10411 DsbC_N [H]

EC number: NA

Molecular weight: Translated: 25926; Mature: 25926

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIRIAALALAVATLGLGCSAQADQTTDKLKSTLQARLSEATIKNVSKSPIDGLYEVN
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
LGSQIIYSDASGDHLILGDMIDAKTRKNLTEARLSETNRIDFASLPFANAVKVVKGNGSR
CCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCEEEEECCCCE
KIAVFSDPNCPYCKQLETTLKSMDNITVYTFLYPVLSPDSTVKSKSIWCSADRAKAWESW
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCEEECCHHHHHHHHH
MQDHRAPTAPGTCDTAAIDRNLALGQSMNVSGTPTVFLADGRRLPGAVPADELDKALSSV
HHHCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHC
R
C
>Mature Secondary Structure
MKKSIRIAALALAVATLGLGCSAQADQTTDKLKSTLQARLSEATIKNVSKSPIDGLYEVN
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
LGSQIIYSDASGDHLILGDMIDAKTRKNLTEARLSETNRIDFASLPFANAVKVVKGNGSR
CCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCEEEEECCCCE
KIAVFSDPNCPYCKQLETTLKSMDNITVYTFLYPVLSPDSTVKSKSIWCSADRAKAWESW
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCEEECCHHHHHHHHH
MQDHRAPTAPGTCDTAAIDRNLALGQSMNVSGTPTVFLADGRRLPGAVPADELDKALSSV
HHHCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]