BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is fadB1 [H]

Identifier: 186477436

GI number: 186477436

Start: 3040821

End: 3041597

Strand: Reverse

Name: fadB1 [H]

Synonym: Bphy_2688

Alternate gene names: 186477436

Gene position: 3041597-3040821 (Counterclockwise)

Preceding gene: 186477442

Following gene: 186477434

Centisome position: 87.42

GC content: 57.27

Gene sequence:

>777_bases
ATGGCATTCGAAAACATTCTGGTTGAGACGCGCGGCCGCGTCGGGTTGATTACGCTGAATCGTCCGAAGGCGTTGAACGC
GCTTAACGATGCTCTCATGGACGAATTGGGAGCGGCGCTACGCGCGTTCGACTCGGATGAAAACATTGGCGCACTTGTCG
TAACCGGGAGCGAAAAGGCATTTGCGGCGGGTGCAGACATCGGAATGATGGCGACGTATTCCTATATGGATGTCTATAAG
GGCGACTACATCACCAGGAACTGGGAGGCAATCCGGTCAGTTCGCAAGCCGATCATTGCGGCGGTAGCCGGGTTCGCATT
GGGTGGCGGCTGCGAGCTCGCGATGATGTGCGACATCATCTTTGCGGCCGACACCGCCAAGTTCGGTCAACCGGAAATCA
AGCTGGGCATCATGCCGGGAGCAGGTGGAACGCAACGCTTGCCGCGTGCGGTATCAAAGGCCAAAGCAATGGATATGTGC
TTGACTGCACGTTTCATGGATGCCGCCGAGGCTGAGCGATCGGGATTGGTGTCGCGCGTGATTCCCGCTGCGGATCTGAT
TGATGAGGCAATTGCTGCGGCCGCGACGATCGCAGAGTTTCCAATTCCGGCAGTGATGATGGTGAAGGAGTCGGTGAATC
GCGCGTATGAGACGACGCTGGCCGAGGGCGTGCATTTCGAGCGCAGGCTGTTTCATTCGCTGTTCGCGACTGAGGATCAG
AAAGAAGGGATGGCGGCCTTCGTTGAGAAGCGCAAGCCGGTTTTCAAGCATCGTTGA

Upstream 100 bases:

>100_bases
TCGTCGATTTATTGACCGACCGGTTGGTTGGTGAATGTTAGCATTATTGGGATGCGGCGCGAGCCCCTTGCGTCTGCAGG
TAAACACTTAGGAGGAGTGC

Downstream 100 bases:

>100_bases
TGGAACTCGTCAAAAAAGCGCTTGCAAGTGCTCACGCGAGCGCCTAGAATTCCGCTCTTTCGCGCTGCGGACAACGTGGC
GCGGGAGAAGCGGAGAAGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAFENILVETRGRVGLITLNRPKALNALNDALMDELGAALRAFDSDENIGALVVTGSEKAFAAGADIGMMATYSYMDVYK
GDYITRNWEAIRSVRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERSGLVSRVIPAADLIDEAIAAAATIAEFPIPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAFENILVETRGRVGLITLNRPKALNALNDALMDELGAALRAFDSDENIGALVVTGSEKAFAAGADIGMMATYSYMDVYK
GDYITRNWEAIRSVRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERSGLVSRVIPAADLIDEAIAAAATIAEFPIPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AFENILVETRGRVGLITLNRPKALNALNDALMDELGAALRAFDSDENIGALVVTGSEKAFAAGADIGMMATYSYMDVYKG
DYITRNWEAIRSVRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDMCL
TARFMDAAEAERSGLVSRVIPAADLIDEAIAAAATIAEFPIPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=55.5984555984556, Blast_Score=288, Evalue=3e-78,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=36.328125, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=29.9180327868852, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=34.4827586206897, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=35.5932203389831, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.9672131147541, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=214, Percent_Identity=28.0373831775701, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594469, Length=214, Percent_Identity=28.0373831775701, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=22.5190839694656, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=22.5190839694656, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=35.1851851851852, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=197, Percent_Identity=25.8883248730964, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=24.4131455399061, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=24.4131455399061, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.2687747035573, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=37.3076923076923, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=34.7169811320755, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=33.0769230769231, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=30, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=30.9178743961353, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=30.5555555555556, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=54.9800796812749, Blast_Score=286, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.0864197530864, Blast_Score=260, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=32.9365079365079, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.3636363636364, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.4894514767932, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=37.696335078534, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=37.7659574468085, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=37.7659574468085, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=37.7659574468085, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.5625, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=28.3018867924528, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=30.9278350515464, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=186, Percent_Identity=31.7204301075269, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=27.8350515463918, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=56.5891472868217, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=56.5891472868217, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=37.593984962406, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=30, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=31.4553990610329, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=32.9411764705882, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654903, Length=242, Percent_Identity=26.4462809917355, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571729, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=239, Percent_Identity=26.3598326359833, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27860; Mature: 27728

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFENILVETRGRVGLITLNRPKALNALNDALMDELGAALRAFDSDENIGALVVTGSEKA
CCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVYKGDYITRNWEAIRSVRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERSGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAADLIDEAIAAAATIAEFPIPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AFENILVETRGRVGLITLNRPKALNALNDALMDELGAALRAFDSDENIGALVVTGSEKA
CCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMATYSYMDVYKGDYITRNWEAIRSVRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERSGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAADLIDEAIAAAATIAEFPIPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]