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The map label for this gene is yoaE [H]

Identifier: 186477434

GI number: 186477434

Start: 3031397

End: 3033475

Strand: Reverse

Name: yoaE [H]

Synonym: Bphy_2686

Alternate gene names: 186477434

Gene position: 3033475-3031397 (Counterclockwise)

Preceding gene: 186477436

Following gene: 186477433

Centisome position: 87.19

GC content: 64.31

Gene sequence:

>2079_bases
ATGAACGCTCCCGTTGAATTTGCCCGCGCCGTCTGCCCGCACGATTGCCCGGATACCTGCGCGATGCGCGTGACGGTCCA
GGGCGGTCGTGCGATCAAGGTCGTCGGCGATCCGGACCATCCGCCCACCCAAGGCGTGCTGTGCACGAAGGTCAGCCGTT
ATGCAGATCGCGTTCACCACCCTCGCCGGCTGACGACTCCGATGCGGCGCGTCGGTCCCAAGGGCGAGGGGCGTTTCGAG
CCGATCAGCTGGGACGAAGCGCTGCATCTGGCAGGCGAGCGCCTGTCGGAAATCGCCGCCCGCGCGCCCGAAGCAATCTT
GCCGTACAGCTACGCCGGCACGATGGGTTTCGTGCAGGGCGACAGCATCGCGCAACGGTTTTTCCATAAGCTCGGTGCGT
CTCAACTCGACCGGACGATCTGCGCAGCGGCCGGCGCGGCGGGCCTCAAGTACACGTATGGCGCGAGTCTCGGCATGCAC
ACCGAGTTTTTCGCCGAGAGCGAACTGATTCTGATCTGGGGCGCAAACCCGATCGCCTCCAACCTGCATTTCTGGACGCG
CGCACAGGAAGCCAAGCGCAACGGCGCGCGCCTCATCGCGATCGATCCGTATCGCTCGCTGACGGCCGAAAAGTGCCACC
AGCACATCGCGCTGAGGCCGGGCACGGACGGCGCGCTCGCGCTCGGCATGATGCATGTGCTGATCGCCGAAGAGATGCTC
GATCGCGCATATATCGCCGATCACACGCTCGGTTTCGAGCAACTCAACGCGCGCGCGCTCGCCTATCCGCCTTCGCGAGC
CGCGCAGATTTGCGGCATCGAAGAACAGGTAATCGTCGATCTCGCGCGTCTTTATGGCCGCACAAAAAAGGCGGCAATCC
GTTTGAACTACGGGATGCAACGGGTGCGCGGCGGCGGCAATGCCGTGCGCGCGATCGCCTGTCTGCCTTCGCTGACGGGC
GCATGGCGCGAGCGCGCGGGCGGAATGCTGCTGTCGTCGTCGGGCTGGGCGCCCGTCGATTCACACGCGCTTTCGCGTCC
CGATCTGATGCCGGGCTGGCCGTCGCAGCCGGCGCGTGTCATCAACATGAACGCAATCGGTGATGCGCTGTGCCATCCGG
GCGATGCGACTTTTGGTCCGAAGATCGAGGCAATCGTCGTGTACAACTCAAACCCCGTGGCCGTGGCGCCCGACTCGGCG
CGGGTCGCGTCCGGTTTCGCGCGCGAGGATCTGTTCACGATCGTTCTCGAGCACTTCCAGACGGATACGGCCGACTACGC
AGACTTACTGCTGCCGGCGACGACCCAGCTCGAGCATCTGGATATTCACAAGTCGTACGGCCACACGCACGTGATGGTGA
ATCTGCCCGCGATCGCACCCGTTGGAGAAGCGCGTCCGAATACGGAGATCTTCCGCGGCATCGCGCGGTCCATGAACCTC
GACGAACCGGCGTTGTACGACAGCGACGAGACGGTAGCGAAGTCGGCCTTGCGGTGGAATGACCCGCTGCTGGAAGGCGT
CACGTGGGACACACTCAAGCGTGACGGCTGGGCGAAGCTGAAGGTGTCGGATGCACCGTTCGCAAACGGCGGATTCCGCA
CGCCGTCGGGCAAGTGCGAGTTTTTCAGCGAGCGTCTCGCGCAGGCGGGCCTCGAGCCCGTACCGGATTATCTGCCGCCG
TACGAATCGGCGGACGGCTCGCCGGATCTCGCCGAACGCTATCCGCTCGCGATGATTTCCCCGCCCGCACGCAATTTCCT
CAACAGCACCTTCGTCAACGTCGACAGCCTGCGCGCGACGGAAGGCGAGCCGCACCTCGACATGCATCCCGCAGACGCAT
GTGAACGTGGCATCAACGACGGTGATCAGGTTCGCATCTTCAATGATCGCGGCTCGATGCAGGCTCGCGTCCGTGTCACC
GGGAAATCCCGCGAAGGGCTCGTCGTCGGTCTGTCAATCTGGTGGAAGAAGCTCGCGCCGGACGGCCGAAATGCCAACGA
GGTCACAAGCCAGGCGCTGACCGACCTCGGCGGTTCGGCGACTTTCTACGACTGCCTCGTCGAAGTCGAACGCGCCTGA

Upstream 100 bases:

>100_bases
TACGCCGATCCCCATTTTTGACGGCCATGCGTCAAATCTGTTCGCCAGAACGTAATAAAATCAATACCTTCATCCGCGCT
GTCCCTGTCGATATAAACAC

Downstream 100 bases:

>100_bases
GCCATTTTCCGAGCGCGCGGCGGATCCTCCTCCGCGCCACCGCGCCACCGCGCCCCTGTCCGCGCTTTCGAGTGCATCGT
CTAACTGGTGGCCTATCCCG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MNAPVEFARAVCPHDCPDTCAMRVTVQGGRAIKVVGDPDHPPTQGVLCTKVSRYADRVHHPRRLTTPMRRVGPKGEGRFE
PISWDEALHLAGERLSEIAARAPEAILPYSYAGTMGFVQGDSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMH
TEFFAESELILIWGANPIASNLHFWTRAQEAKRNGARLIAIDPYRSLTAEKCHQHIALRPGTDGALALGMMHVLIAEEML
DRAYIADHTLGFEQLNARALAYPPSRAAQICGIEEQVIVDLARLYGRTKKAAIRLNYGMQRVRGGGNAVRAIACLPSLTG
AWRERAGGMLLSSSGWAPVDSHALSRPDLMPGWPSQPARVINMNAIGDALCHPGDATFGPKIEAIVVYNSNPVAVAPDSA
RVASGFAREDLFTIVLEHFQTDTADYADLLLPATTQLEHLDIHKSYGHTHVMVNLPAIAPVGEARPNTEIFRGIARSMNL
DEPALYDSDETVAKSALRWNDPLLEGVTWDTLKRDGWAKLKVSDAPFANGGFRTPSGKCEFFSERLAQAGLEPVPDYLPP
YESADGSPDLAERYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDMHPADACERGINDGDQVRIFNDRGSMQARVRVT
GKSREGLVVGLSIWWKKLAPDGRNANEVTSQALTDLGGSATFYDCLVEVERA

Sequences:

>Translated_692_residues
MNAPVEFARAVCPHDCPDTCAMRVTVQGGRAIKVVGDPDHPPTQGVLCTKVSRYADRVHHPRRLTTPMRRVGPKGEGRFE
PISWDEALHLAGERLSEIAARAPEAILPYSYAGTMGFVQGDSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMH
TEFFAESELILIWGANPIASNLHFWTRAQEAKRNGARLIAIDPYRSLTAEKCHQHIALRPGTDGALALGMMHVLIAEEML
DRAYIADHTLGFEQLNARALAYPPSRAAQICGIEEQVIVDLARLYGRTKKAAIRLNYGMQRVRGGGNAVRAIACLPSLTG
AWRERAGGMLLSSSGWAPVDSHALSRPDLMPGWPSQPARVINMNAIGDALCHPGDATFGPKIEAIVVYNSNPVAVAPDSA
RVASGFAREDLFTIVLEHFQTDTADYADLLLPATTQLEHLDIHKSYGHTHVMVNLPAIAPVGEARPNTEIFRGIARSMNL
DEPALYDSDETVAKSALRWNDPLLEGVTWDTLKRDGWAKLKVSDAPFANGGFRTPSGKCEFFSERLAQAGLEPVPDYLPP
YESADGSPDLAERYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDMHPADACERGINDGDQVRIFNDRGSMQARVRVT
GKSREGLVVGLSIWWKKLAPDGRNANEVTSQALTDLGGSATFYDCLVEVERA
>Mature_692_residues
MNAPVEFARAVCPHDCPDTCAMRVTVQGGRAIKVVGDPDHPPTQGVLCTKVSRYADRVHHPRRLTTPMRRVGPKGEGRFE
PISWDEALHLAGERLSEIAARAPEAILPYSYAGTMGFVQGDSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMH
TEFFAESELILIWGANPIASNLHFWTRAQEAKRNGARLIAIDPYRSLTAEKCHQHIALRPGTDGALALGMMHVLIAEEML
DRAYIADHTLGFEQLNARALAYPPSRAAQICGIEEQVIVDLARLYGRTKKAAIRLNYGMQRVRGGGNAVRAIACLPSLTG
AWRERAGGMLLSSSGWAPVDSHALSRPDLMPGWPSQPARVINMNAIGDALCHPGDATFGPKIEAIVVYNSNPVAVAPDSA
RVASGFAREDLFTIVLEHFQTDTADYADLLLPATTQLEHLDIHKSYGHTHVMVNLPAIAPVGEARPNTEIFRGIARSMNL
DEPALYDSDETVAKSALRWNDPLLEGVTWDTLKRDGWAKLKVSDAPFANGGFRTPSGKCEFFSERLAQAGLEPVPDYLPP
YESADGSPDLAERYPLAMISPPARNFLNSTFVNVDSLRATEGEPHLDMHPADACERGINDGDQVRIFNDRGSMQARVRVT
GKSREGLVVGLSIWWKKLAPDGRNANEVTSQALTDLGGSATFYDCLVEVERA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=772, Percent_Identity=26.8134715025907, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI171474008, Length=771, Percent_Identity=27.7561608300908, Blast_Score=211, Evalue=2e-55,
Organism=Escherichia coli, GI1787870, Length=765, Percent_Identity=27.4509803921569, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI3868721, Length=719, Percent_Identity=26.2865090403338, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081994, Length=712, Percent_Identity=25.9831460674157, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI145693196, Length=677, Percent_Identity=26.7355982274741, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1787231, Length=733, Percent_Identity=23.7380627557981, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788534, Length=332, Percent_Identity=20.4819277108434, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI3868720, Length=263, Percent_Identity=27.3764258555133, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75369; Mature: 75369

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVEFARAVCPHDCPDTCAMRVTVQGGRAIKVVGDPDHPPTQGVLCTKVSRYADRVHH
CCCCHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
PRRLTTPMRRVGPKGEGRFEPISWDEALHLAGERLSEIAARAPEAILPYSYAGTMGFVQG
CHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC
DSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMHTEFFAESELILIWGANPIAS
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCCEEEEECCCHHHC
NLHFWTRAQEAKRNGARLIAIDPYRSLTAEKCHQHIALRPGTDGALALGMMHVLIAEEML
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DRAYIADHTLGFEQLNARALAYPPSRAAQICGIEEQVIVDLARLYGRTKKAAIRLNYGMQ
HHHHHHHCCCCHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHCCCHHEEEEECCCHH
RVRGGGNAVRAIACLPSLTGAWRERAGGMLLSSSGWAPVDSHALSRPDLMPGWPSQPARV
HHCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCEE
INMNAIGDALCHPGDATFGPKIEAIVVYNSNPVAVAPDSARVASGFAREDLFTIVLEHFQ
EEECHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCHHHHHCHHHHHHHHHHHHHHC
TDTADYADLLLPATTQLEHLDIHKSYGHTHVMVNLPAIAPVGEARPNTEIFRGIARSMNL
CCCCHHHHHHCCCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYDSDETVAKSALRWNDPLLEGVTWDTLKRDGWAKLKVSDAPFANGGFRTPSGKCE
CCCCCCCCCHHHHHHHHHCCCHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHH
FFSERLAQAGLEPVPDYLPPYESADGSPDLAERYPLAMISPPARNFLNSTFVNVDSLRAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHCCHHHCCCC
EGEPHLDMHPADACERGINDGDQVRIFNDRGSMQARVRVTGKSREGLVVGLSIWWKKLAP
CCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEHHHHHHHHCC
DGRNANEVTSQALTDLGGSATFYDCLVEVERA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNAPVEFARAVCPHDCPDTCAMRVTVQGGRAIKVVGDPDHPPTQGVLCTKVSRYADRVHH
CCCCHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
PRRLTTPMRRVGPKGEGRFEPISWDEALHLAGERLSEIAARAPEAILPYSYAGTMGFVQG
CHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC
DSIAQRFFHKLGASQLDRTICAAAGAAGLKYTYGASLGMHTEFFAESELILIWGANPIAS
HHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCCEEEEECCCHHHC
NLHFWTRAQEAKRNGARLIAIDPYRSLTAEKCHQHIALRPGTDGALALGMMHVLIAEEML
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DRAYIADHTLGFEQLNARALAYPPSRAAQICGIEEQVIVDLARLYGRTKKAAIRLNYGMQ
HHHHHHHCCCCHHHHCCCEEECCCCHHHHHCCCHHHHHHHHHHHHCCCHHEEEEECCCHH
RVRGGGNAVRAIACLPSLTGAWRERAGGMLLSSSGWAPVDSHALSRPDLMPGWPSQPARV
HHCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCEE
INMNAIGDALCHPGDATFGPKIEAIVVYNSNPVAVAPDSARVASGFAREDLFTIVLEHFQ
EEECHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCHHHHHCHHHHHHHHHHHHHHC
TDTADYADLLLPATTQLEHLDIHKSYGHTHVMVNLPAIAPVGEARPNTEIFRGIARSMNL
CCCCHHHHHHCCCCCCHHHEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYDSDETVAKSALRWNDPLLEGVTWDTLKRDGWAKLKVSDAPFANGGFRTPSGKCE
CCCCCCCCCHHHHHHHHHCCCHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHH
FFSERLAQAGLEPVPDYLPPYESADGSPDLAERYPLAMISPPARNFLNSTFVNVDSLRAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHCCHHHCCCC
EGEPHLDMHPADACERGINDGDQVRIFNDRGSMQARVRVTGKSREGLVVGLSIWWKKLAP
CCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEHHHHHHHHCC
DGRNANEVTSQALTDLGGSATFYDCLVEVERA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]