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The map label for this gene is mutT [H]

Identifier: 186477409

GI number: 186477409

Start: 3001029

End: 3001472

Strand: Reverse

Name: mutT [H]

Synonym: Bphy_2661

Alternate gene names: 186477409

Gene position: 3001472-3001029 (Counterclockwise)

Preceding gene: 186477410

Following gene: 186477400

Centisome position: 86.27

GC content: 66.44

Gene sequence:

>444_bases
ATGAGCGACGCAGCGCATAACGGCGCGCCGAAGGCCGCAGACGTCAACGAAGCGGGCCGGCCGGTGACGGAAGTGGCCGT
CGGCGTGCTTGTTCGACCCGACGGCCGCTATCTGCTTGCGCAAAGGCCCGCGGGAAAACCGTATGAAGGCTACTGGGAGT
TTCCGGGCGGCAAGCTGGAGCCGGGCGAATCGGTCGAAGCGGCGCTTGCACGCGAACTGCACGAGGAACTCGGCATCGAC
GTGCAGTCTAGCCATCGCTGGCACGTGCTCGAGCACGACTATCCGCACGCGTACGTGCGCCTCTATTTCTGCAAGGTGAC
CGCCTGGGGCGGCGAGCCGCATGGCCGCGAAGGTCAGGCGTTCGCGTGGCAGACGTTGCCCGCTCAGGTGTCGCCGTTGC
TGCCTGCAACGATCCCCGTGCTGGAGTGGCTGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
CGCGCGGTGACGCGTTGATCTGGGCGTTGGAGCGCGGTTCGCGCTCCGGACGCGTCGCATGGCAGTTCGCGCGCGACTGG
TCGGGCAAGCGGGCGCAAGC

Downstream 100 bases:

>100_bases
AACGAAGGAGGAATCGCCGTATGCGGCTTCCGCCTTTTTCGATGTGCAACGCCCTGCGGTCGCCGTTCAGTGCGAGCGGT
GTTCGCCGGGTGTGTCGTCG

Product: NUDIX hydrolase

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSDAAHNGAPKAADVNEAGRPVTEVAVGVLVRPDGRYLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGID
VQSSHRWHVLEHDYPHAYVRLYFCKVTAWGGEPHGREGQAFAWQTLPAQVSPLLPATIPVLEWLAAE

Sequences:

>Translated_147_residues
MSDAAHNGAPKAADVNEAGRPVTEVAVGVLVRPDGRYLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGID
VQSSHRWHVLEHDYPHAYVRLYFCKVTAWGGEPHGREGQAFAWQTLPAQVSPLLPATIPVLEWLAAE
>Mature_146_residues
SDAAHNGAPKAADVNEAGRPVTEVAVGVLVRPDGRYLLAQRPAGKPYEGYWEFPGGKLEPGESVEAALARELHEELGIDV
QSSHRWHVLEHDYPHAYVRLYFCKVTAWGGEPHGREGQAFAWQTLPAQVSPLLPATIPVLEWLAAE

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=119, Percent_Identity=36.9747899159664, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1788056, Length=121, Percent_Identity=38.8429752066116, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16129; Mature: 15998

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAHNGAPKAADVNEAGRPVTEVAVGVLVRPDGRYLLAQRPAGKPYEGYWEFPGGKLE
CCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCC
PGESVEAALARELHEELGIDVQSSHRWHVLEHDYPHAYVRLYFCKVTAWGGEPHGREGQA
CCCHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCE
FAWQTLPAQVSPLLPATIPVLEWLAAE
EEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SDAAHNGAPKAADVNEAGRPVTEVAVGVLVRPDGRYLLAQRPAGKPYEGYWEFPGGKLE
CCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCC
PGESVEAALARELHEELGIDVQSSHRWHVLEHDYPHAYVRLYFCKVTAWGGEPHGREGQA
CCCHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCE
FAWQTLPAQVSPLLPATIPVLEWLAAE
EEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8244038 [H]