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The map label for this gene is proB [H]

Identifier: 186477397

GI number: 186477397

Start: 2989705

End: 2990823

Strand: Reverse

Name: proB [H]

Synonym: Bphy_2649

Alternate gene names: 186477397

Gene position: 2990823-2989705 (Counterclockwise)

Preceding gene: 186477398

Following gene: 186477394

Centisome position: 85.96

GC content: 63.72

Gene sequence:

>1119_bases
ATGCGTTCCGTTATCGCAGATTCCAGGCGATTGGTCGTGAAAGTCGGTTCCAGTCTCGTCACGAACGACGGGCGGGGACT
CGATCACACTGCAATCGGCCGCTGGGCCGCGCAGATCGCCGCACTGCGCGAGCAGGGCAAGGAAGTCGTGCTCGTCAGTT
CGGGCGCCATTGCTGAAGGTATGCAGCGTCTTGGTTGGACCAGGCGCCCGCGCGAAATCGACGAACTGCAGGCGGCTGCG
GCCGTCGGGCAGATGGGGCTCGCCCAAGTCTACGAAAGCCGCTTTTCCGAGCATTCCATCCGTACCGCGCAAATCCTGTT
GACGCACGCCGATCTTGCCGATCGTGAACGCTATCTGAACGCGCGCTCCACGCTGCTGACGCTGCTGCGACTCGGTGTGG
TGCCCATCATTAACGAAAACGACACTGTCGTGACCGATGAGATCAAGTTCGGCGACAACGATACGCTAGGTGCGCTGGTT
GCGAATCTGATCGAAGGTGATGCGCTTATCATCCTCACCGACCAGCAAGGTCTCTTCACCGCCGATCCTCGCAAGGATCC
GGCCGCCACGCTGGTTCAGCAGGCCGATGCCGGCACGCCGGAACTTGAGGCGATGGCGGGCGGCGCGGGTTCGAGCATCG
GCCGCGGCGGCATGCTCACGAAAATCCTGGCGGCGAAGCGCGCGGCGCACAGTGGCGCGAACACCGTGATCGCGAGCGGC
CGCGAGGCGGACATATTGGCGCGGCTCGCATCGGGCGAGGCGATCGGCACGCAACTGATCGCGCGAACGGCACGCATGGC
CGCCCGCAAACAGTGGATGGCGGATCACCTGCAGGTGCGGGGGCATGTGGTCATTGACGACGGCGCTGTCGAAAAGCTCA
CGGAAGGCGGGAAGAGCCTGTTGCCGATCGGTGTGGTCGGCGTGCAGGGTGCGTTTGCGCGCGGCGAGGTGATCGCGTGC
CTGAACGCGGCTGGGCGTGAAGTCGCTCGCGGGCTGACGAACTACAGCAGTGCAGAGACGAAGCTGATCCAACGTCGTCC
GAGCGGCGAGATCGAATCCGTCTTGGGTTATATGCTCGAGCCGGAGCTGATCCACCGGGACAATCTGGTGCTCGTCTAG

Upstream 100 bases:

>100_bases
CGCCCGTCGGGCCGCAGGAGTAAGCGAGAGCCGTAATCTGGATGCCGGCGCACTTCCCAGCATGCGCCGGTCTTACGAAT
CATCTTGGAGACCGCGCACA

Downstream 100 bases:

>100_bases
CGAACCCTTCCACTGTCGAAAATCAAAAAGCCGTCCCGTAAACGATGGGACGGCTTTTTGTTGCCGCTGCATCGAGCGGC
GCCTGGATCTAGCTGCCGCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSVIADSRRLVVKVGSSLVTNDGRGLDHTAIGRWAAQIAALREQGKEVVLVSSGAIAEGMQRLGWTRRPREIDELQAAA
AVGQMGLAQVYESRFSEHSIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQQGLFTADPRKDPAATLVQQADAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
READILARLASGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDDGAVEKLTEGGKSLLPIGVVGVQGAFARGEVIAC
LNAAGREVARGLTNYSSAETKLIQRRPSGEIESVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSVIADSRRLVVKVGSSLVTNDGRGLDHTAIGRWAAQIAALREQGKEVVLVSSGAIAEGMQRLGWTRRPREIDELQAAA
AVGQMGLAQVYESRFSEHSIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQQGLFTADPRKDPAATLVQQADAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
READILARLASGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDDGAVEKLTEGGKSLLPIGVVGVQGAFARGEVIAC
LNAAGREVARGLTNYSSAETKLIQRRPSGEIESVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSVIADSRRLVVKVGSSLVTNDGRGLDHTAIGRWAAQIAALREQGKEVVLVSSGAIAEGMQRLGWTRRPREIDELQAAA
AVGQMGLAQVYESRFSEHSIRTAQILLTHADLADRERYLNARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQQGLFTADPRKDPAATLVQQADAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
READILARLASGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDDGAVEKLTEGGKSLLPIGVVGVQGAFARGEVIAC
LNAAGREVARGLTNYSSAETKLIQRRPSGEIESVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=35.8361774744027, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=35.5932203389831, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.1693989071038, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17569967, Length=278, Percent_Identity=34.1726618705036, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=278, Percent_Identity=34.1726618705036, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=422, Percent_Identity=34.5971563981043, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=31.1594202898551, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=34.020618556701, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39538; Mature: 39538

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVIADSRRLVVKVGSSLVTNDGRGLDHTAIGRWAAQIAALREQGKEVVLVSSGAIAEG
CCCCHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWTRRPREIDELQAAAAVGQMGLAQVYESRFSEHSIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQQGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPAATLVQQADAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
READILARLASGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDDGAVEKLTEGGKSL
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCC
LPIGVVGVQGAFARGEVIACLNAAGREVARGLTNYSSAETKLIQRRPSGEIESVLGYMLE
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSVIADSRRLVVKVGSSLVTNDGRGLDHTAIGRWAAQIAALREQGKEVVLVSSGAIAEG
CCCCHHCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWTRRPREIDELQAAAAVGQMGLAQVYESRFSEHSIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
ARSTLLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQQGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPAATLVQQADAGTPELEAMAGGAGSSIGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
READILARLASGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDDGAVEKLTEGGKSL
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCC
LPIGVVGVQGAFARGEVIACLNAAGREVARGLTNYSSAETKLIQRRPSGEIESVLGYMLE
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA