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The map label for this gene is rppH

Identifier: 186477395

GI number: 186477395

Start: 2988267

End: 2988962

Strand: Direct

Name: rppH

Synonym: Bphy_2647

Alternate gene names: 186477395

Gene position: 2988267-2988962 (Clockwise)

Preceding gene: 186477392

Following gene: 186477396

Centisome position: 85.89

GC content: 61.49

Gene sequence:

>696_bases
ATGCTGGATCGTGAAGGCTTTCGCCCGAACGTCGGCATCATCCTCTTGAACGCTCACAACGAGGTGTTTTGGGGCAAGCG
TCTACGTGAACATTCCTGGCAGTTTCCGCAAGGGGGCATCAAGTATGGTGAGACCCCTGTGCAGGCGATGTATCGGGAGT
TGCACGAAGAGACCGGCCTGCTTCCGGAGCACGTCAAGGTTATCGGTCGCACGCGCGACTGGTTGCGTTATGAGGTGCCT
GACAAGTTCATCAAGCGTGAGGTCCGCGGCCACTACCGCGGTCAGAAGCAGATCTGGTTCCTGCTCCGGATGGTTGGACG
CGATTGCGACATATGTCTGCGCGCGACCGATCACCCTGAGTTCGATGCCTGGCGCTGGAACGAGTACTGGGTGCCGCTCG
ACTGTGTGATCGAGTTCAAGCGGGATGTGTATCAGCTGGCGTTAACGGAGTTGTCCCGCTTCCTGCGGCGGCCTCAGCCG
CGCACGGAAAGGCCCGGTGGGCATCATCACGGGCAGCGCTATCCGCGGATGGCGTCGTCGGTGAATGCGCCACCCGGGGC
GTCGATGGCGTCGTCCATGACGGTGACCACCGTCACGACGGCAATTGCAGTCGAGTCGACGCTGCGTGCGACAATCGAAT
CGGACTGTTCGTCGACGGAAGACCCGGCCGTTCAGGCTCCGGGGCTGCGCGACTGA

Upstream 100 bases:

>100_bases
GGTTGTTACGAGCAATCGCGCCAATCTGGTGCGAGGCGGTGCGTCAGACCGGTTATCTGTTTATAATCAAAGCAATTTTA
AAGGATTCGAAGGTGGTTGT

Downstream 100 bases:

>100_bases
CGCATAGCGGCTGTCCACGCCGCATCTGCCGGTGCGGCGTGAGATACGCTGCGCCGGCGTTTCGAGGGAATCACATTGAA
AGTATCTGCTTTCGCCGTGG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLDREGFRPNVGIILLNAHNEVFWGKRLREHSWQFPQGGIKYGETPVQAMYRELHEETGLLPEHVKVIGRTRDWLRYEVP
DKFIKREVRGHYRGQKQIWFLLRMVGRDCDICLRATDHPEFDAWRWNEYWVPLDCVIEFKRDVYQLALTELSRFLRRPQP
RTERPGGHHHGQRYPRMASSVNAPPGASMASSMTVTTVTTAIAVESTLRATIESDCSSTEDPAVQAPGLRD

Sequences:

>Translated_231_residues
MLDREGFRPNVGIILLNAHNEVFWGKRLREHSWQFPQGGIKYGETPVQAMYRELHEETGLLPEHVKVIGRTRDWLRYEVP
DKFIKREVRGHYRGQKQIWFLLRMVGRDCDICLRATDHPEFDAWRWNEYWVPLDCVIEFKRDVYQLALTELSRFLRRPQP
RTERPGGHHHGQRYPRMASSVNAPPGASMASSMTVTTVTTAIAVESTLRATIESDCSSTEDPAVQAPGLRD
>Mature_231_residues
MLDREGFRPNVGIILLNAHNEVFWGKRLREHSWQFPQGGIKYGETPVQAMYRELHEETGLLPEHVKVIGRTRDWLRYEVP
DKFIKREVRGHYRGQKQIWFLLRMVGRDCDICLRATDHPEFDAWRWNEYWVPLDCVIEFKRDVYQLALTELSRFLRRPQP
RTERPGGHHHGQRYPRMASSVNAPPGASMASSMTVTTVTTAIAVESTLRATIESDCSSTEDPAVQAPGLRD

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789194, Length=165, Percent_Identity=46.0606060606061, Blast_Score=173, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPPH_BURP8 (B2JHD4)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858865.1
- ProteinModelPortal:   B2JHD4
- SMR:   B2JHD4
- GeneID:   6244130
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2647
- HOGENOM:   HBG302451
- OMA:   GQKQIWY
- ProtClustDB:   CLSK2314437
- HAMAP:   MF_00298
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.- [C]

Molecular weight: Translated: 26799; Mature: 26799

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDREGFRPNVGIILLNAHNEVFWGKRLREHSWQFPQGGIKYGETPVQAMYRELHEETGL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
LPEHVKVIGRTRDWLRYEVPDKFIKREVRGHYRGQKQIWFLLRMVGRDCDICLRATDHPE
CHHHHHHHHCCHHHHEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCC
FDAWRWNEYWVPLDCVIEFKRDVYQLALTELSRFLRRPQPRTERPGGHHHGQRYPRMASS
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
VNAPPGASMASSMTVTTVTTAIAVESTLRATIESDCSSTEDPAVQAPGLRD
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDREGFRPNVGIILLNAHNEVFWGKRLREHSWQFPQGGIKYGETPVQAMYRELHEETGL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
LPEHVKVIGRTRDWLRYEVPDKFIKREVRGHYRGQKQIWFLLRMVGRDCDICLRATDHPE
CHHHHHHHHCCHHHHEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCC
FDAWRWNEYWVPLDCVIEFKRDVYQLALTELSRFLRRPQPRTERPGGHHHGQRYPRMASS
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
VNAPPGASMASSMTVTTVTTAIAVESTLRATIESDCSSTEDPAVQAPGLRD
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA