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The map label for this gene is tilS/hprT [H]

Identifier: 186477392

GI number: 186477392

Start: 2984997

End: 2985548

Strand: Direct

Name: tilS/hprT [H]

Synonym: Bphy_2644

Alternate gene names: 186477392

Gene position: 2984997-2985548 (Clockwise)

Preceding gene: 186477391

Following gene: 186477395

Centisome position: 85.8

GC content: 62.5

Gene sequence:

>552_bases
ATGAATCGCGAAGAAGCTCTCCACATTTTCAGTCACTCCGAAGAAATCGTTTCGGCCACCGAGGTCAACGCATCCATCGG
CAGCATGGCGGACGCAATCCGCGCCGAGATGGCCGAAGACTTTCCACTCGTGCTGTCGGTGATGGGTGGCGCGGCGGTGT
TCACGGGCATGCTGCTGCCGCACCTCGATTTCCCGCTTGAGTTCGACTACATCCACCTCACCCGCTATCGCAACGCCATC
AAGGGCAGCGCGGAGATGCAGTGGCGCGTCGCGCCGTCGGGCTCGGTGAAGGACCGCGTAGTACTCGTGCTCGACGACAT
TCTCGACGAAGGCGAGACGATGGCCGCGATCCGCGACCGCATCATGGCGATGGGCGCGAAGCGTTTCATGAGCGCGGTGC
TGTGCGAGAAGATGATTCCGAAGGCGAAGCCGCTGCGTCCGGACTTCTGCGGGTTCGAAGTGCCGGACCGCTACGTGTTC
GGCTGCGGAATGGACGCAAAGGGCTACTGGCGCAATCTGCCGACCATCCGCGCACTGACGGAAGGCGCGTGA

Upstream 100 bases:

>100_bases
CGGCCCGGCGCGTATGCCGGATCAAGGCGCGGGTATTTGCTGTAGCGCGCCGGGTCGCTGTCTCCCACACTATGTATACA
GTTATCGCCCGTCAGACGTC

Downstream 100 bases:

>100_bases
TGCAGTTTCTGGCAGCGCTACAGCGCTGCAAAATAAAAAAGCGACCTGCGGGTCGCTTTTTTGTTGGCCGGATGGCCATG
CTTCACTTTTCGAGCTGATG

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNREEALHIFSHSEEIVSATEVNASIGSMADAIRAEMAEDFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNAI
KGSAEMQWRVAPSGSVKDRVVLVLDDILDEGETMAAIRDRIMAMGAKRFMSAVLCEKMIPKAKPLRPDFCGFEVPDRYVF
GCGMDAKGYWRNLPTIRALTEGA

Sequences:

>Translated_183_residues
MNREEALHIFSHSEEIVSATEVNASIGSMADAIRAEMAEDFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNAI
KGSAEMQWRVAPSGSVKDRVVLVLDDILDEGETMAAIRDRIMAMGAKRFMSAVLCEKMIPKAKPLRPDFCGFEVPDRYVF
GCGMDAKGYWRNLPTIRALTEGA
>Mature_183_residues
MNREEALHIFSHSEEIVSATEVNASIGSMADAIRAEMAEDFPLVLSVMGGAAVFTGMLLPHLDFPLEFDYIHLTRYRNAI
KGSAEMQWRVAPSGSVKDRVVLVLDDILDEGETMAAIRDRIMAMGAKRFMSAVLCEKMIPKAKPLRPDFCGFEVPDRYVF
GCGMDAKGYWRNLPTIRALTEGA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=163, Percent_Identity=26.3803680981595, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17510817, Length=159, Percent_Identity=25.7861635220126, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20318; Mature: 20318

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREEALHIFSHSEEIVSATEVNASIGSMADAIRAEMAEDFPLVLSVMGGAAVFTGMLLP
CCHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HLDFPLEFDYIHLTRYRNAIKGSAEMQWRVAPSGSVKDRVVLVLDDILDEGETMAAIRDR
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEHHHHCCCHHHHHHHHH
IMAMGAKRFMSAVLCEKMIPKAKPLRPDFCGFEVPDRYVFGCGMDAKGYWRNLPTIRALT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHHC
EGA
CCC
>Mature Secondary Structure
MNREEALHIFSHSEEIVSATEVNASIGSMADAIRAEMAEDFPLVLSVMGGAAVFTGMLLP
CCHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HLDFPLEFDYIHLTRYRNAIKGSAEMQWRVAPSGSVKDRVVLVLDDILDEGETMAAIRDR
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEHHHHCCCHHHHHHHHH
IMAMGAKRFMSAVLCEKMIPKAKPLRPDFCGFEVPDRYVFGCGMDAKGYWRNLPTIRALT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCHHHHHC
EGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]