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The map label for this gene is nrdB [H]

Identifier: 186477386

GI number: 186477386

Start: 2976180

End: 2977403

Strand: Reverse

Name: nrdB [H]

Synonym: Bphy_2638

Alternate gene names: 186477386

Gene position: 2977403-2976180 (Counterclockwise)

Preceding gene: 186477387

Following gene: 186477385

Centisome position: 85.58

GC content: 58.42

Gene sequence:

>1224_bases
ATGCTCAACTGGGATGACGAGATCACTGCCGTAACTCCCTCGAGTGCTTCGCAACAAAACGTGTTGCGCAACGCTGCGGG
AACGGCTGTCGGTTCGCAAGTCGAAACGCGTTCCGCTCATCAAGCTCCCTCGGCTCAACAGGTCTTCGCGAACGACATCG
CAATCGCTCCGCCGGCGCAAGCAGGTGCACCGGCCGTCGCTTCCGAAGCTCGTGTGAATGTCGCCGACAAGCGCATCATC
AACGGCCAGACTGACGTCAATCAGCTGGTGCCGTTCAAGTACAAGTGGGCGTGGGAAAAGTACCTGTCCGGTTGCGCGAA
CCACTGGATGCCGCAGGAAATCAACATGTCCCGCGACATCGCGCTCTGGAAAGACCCGAATGGGCTGACGGAAGACGAGC
GCCGCATCATCAAGCGCAACCTCGGCTTCTTCGTGACGGCCGACTCGCTCGCGGCGAACAACATCGTCCTCGGCACGTAC
CGCCACATCACGGCGCCCGAATGCCGCCAGTTCCTGCTGCGCCAGGCATTTGAAGAGGCGATCCACACACACGCCTATCA
GTACATCGTGGAGTCGCTCGGTCTCGACGAGGGCGAGATCTTCAACGCGTACCACGAAGTCCAGTCCATCCGCGACAAAG
ACGAATTCCTGATTCCGTTCATCCACACGCTGACCGACCCCGCCTTCAAGACGGGTACGCTCGAAGCAGACCAGAAGCTG
CTCAAGTCGCTGATCGTGTTCGCCTGCGTGATGGAAGGCCTGTTCTTCTACGTTGGCTTCACACAGATCCTGGCGCTCGG
CCGCCAGAACAAGATGACGGGCGCGGCGGAACAGTACCAGTACATCCTGCGCGACGAGTCGATGCACTGCAATTTCGGCA
TTGACCTGATCAACCAGATCAAGCTCGAAAACCCGCATCTCTGGACGCCGGAATTCCGCGCCGAGATCCGCGAGATCTTC
AAACACGCGGTCGACCTCGAATATCGCTACGCAGAAGACACGATGCCGCGCGGGGTGCTCGGCCTCAATGCGTCGATGTT
CAAGAGCTATCTGCGCTTTATCTGCAACCGCCGTTGCCAGCAGATCGGTATCGATCCGCTGTATCCGAACGAGGAAAACC
CGTTCCCGTGGATGAGCGAGATGATCGACCTGAAGAAAGAACGCAATTTCTTCGAGACTCGGGTTATCGAATATCAGACC
GGCGGCGCGCTGTCTTGGGAATAA

Upstream 100 bases:

>100_bases
TAGGTCGCAACGCGATTCGAAAACAGGATTTTTCATGAGCGGACCCTTTTAACGCTCGTGAAAAGATGGTACAAACCGAT
CTAAATTGATGGTGACATTT

Downstream 100 bases:

>100_bases
CCCAACGCGTCGTGTATGTATTTGATGGGGCTGCCGCTGGCCGTGCCGCGGCAAGTTTGGTGAGGAGCATGATCGCCTGA
TGCAGAGCGTGCCCGGTGTG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLNWDDEITAVTPSSASQQNVLRNAAGTAVGSQVETRSAHQAPSAQQVFANDIAIAPPAQAGAPAVASEARVNVADKRII
NGQTDVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTY
RHITAPECRQFLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEVQSIRDKDEFLIPFIHTLTDPAFKTGTLEADQKL
LKSLIVFACVMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKLENPHLWTPEFRAEIREIF
KHAVDLEYRYAEDTMPRGVLGLNASMFKSYLRFICNRRCQQIGIDPLYPNEENPFPWMSEMIDLKKERNFFETRVIEYQT
GGALSWE

Sequences:

>Translated_407_residues
MLNWDDEITAVTPSSASQQNVLRNAAGTAVGSQVETRSAHQAPSAQQVFANDIAIAPPAQAGAPAVASEARVNVADKRII
NGQTDVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTY
RHITAPECRQFLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEVQSIRDKDEFLIPFIHTLTDPAFKTGTLEADQKL
LKSLIVFACVMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKLENPHLWTPEFRAEIREIF
KHAVDLEYRYAEDTMPRGVLGLNASMFKSYLRFICNRRCQQIGIDPLYPNEENPFPWMSEMIDLKKERNFFETRVIEYQT
GGALSWE
>Mature_407_residues
MLNWDDEITAVTPSSASQQNVLRNAAGTAVGSQVETRSAHQAPSAQQVFANDIAIAPPAQAGAPAVASEARVNVADKRII
NGQTDVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDIALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTY
RHITAPECRQFLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYHEVQSIRDKDEFLIPFIHTLTDPAFKTGTLEADQKL
LKSLIVFACVMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKLENPHLWTPEFRAEIREIF
KHAVDLEYRYAEDTMPRGVLGLNASMFKSYLRFICNRRCQQIGIDPLYPNEENPFPWMSEMIDLKKERNFFETRVIEYQT
GGALSWE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI4557845, Length=395, Percent_Identity=27.3417721518987, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI260064013, Length=395, Percent_Identity=27.3417721518987, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI289177074, Length=362, Percent_Identity=27.6243093922652, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI42544136, Length=322, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI289177076, Length=298, Percent_Identity=28.8590604026846, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=24.5733788395904, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17551968, Length=351, Percent_Identity=28.4900284900285, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI193206379, Length=304, Percent_Identity=23.0263157894737, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=31.5151515151515, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321619, Length=320, Percent_Identity=23.4375, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18079279, Length=320, Percent_Identity=28.75, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 46471; Mature: 46471

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNWDDEITAVTPSSASQQNVLRNAAGTAVGSQVETRSAHQAPSAQQVFANDIAIAPPAQ
CCCCCCCEEEECCCCCHHHHHHHHHHCHHHCCHHHHHHHHCCCCHHHHHHCCCCCCCCCC
AGAPAVASEARVNVADKRIINGQTDVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDI
CCCCCHHHHCCCCHHHHHHCCCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCE
ALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRHITAPECRQFLLRQAFEEA
EEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
IHTHAYQYIVESLGLDEGEIFNAYHEVQSIRDKDEFLIPFIHTLTDPAFKTGTLEADQKL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LKSLIVFACVMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHE
KLENPHLWTPEFRAEIREIFKHAVDLEYRYAEDTMPRGVLGLNASMFKSYLRFICNRRCQ
ECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHH
QIGIDPLYPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWE
HCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC
>Mature Secondary Structure
MLNWDDEITAVTPSSASQQNVLRNAAGTAVGSQVETRSAHQAPSAQQVFANDIAIAPPAQ
CCCCCCCEEEECCCCCHHHHHHHHHHCHHHCCHHHHHHHHCCCCHHHHHHCCCCCCCCCC
AGAPAVASEARVNVADKRIINGQTDVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDI
CCCCCHHHHCCCCHHHHHHCCCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCE
ALWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRHITAPECRQFLLRQAFEEA
EEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
IHTHAYQYIVESLGLDEGEIFNAYHEVQSIRDKDEFLIPFIHTLTDPAFKTGTLEADQKL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LKSLIVFACVMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHE
KLENPHLWTPEFRAEIREIFKHAVDLEYRYAEDTMPRGVLGLNASMFKSYLRFICNRRCQ
ECCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHH
QIGIDPLYPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWE
HCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]