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The map label for this gene is araF [H]

Identifier: 186477363

GI number: 186477363

Start: 2954109

End: 2955107

Strand: Reverse

Name: araF [H]

Synonym: Bphy_2615

Alternate gene names: 186477363

Gene position: 2955107-2954109 (Counterclockwise)

Preceding gene: 186477364

Following gene: 186477362

Centisome position: 84.94

GC content: 62.46

Gene sequence:

>999_bases
ATGAAACGCAGAATTTTCCTCACGCTCGCAGCAGCGGCGACCGCGGTGCTTTTCAATGCGCCCGTCGCGCAAGCCGCCGA
CACGGTCAAGATCGGCTTCCTCGTGAAGCAGCCCGAAGAGCCGTGGTTTCAGGACGAGTGGAAGTTCGCCGAACAGGCTG
CGAAGGACAAGGGCTTCACGCTGGTGAAGATCGGCGCGCCGTCGGGCGAGAAGGTCATGAGCGCGATCGACAATCTCGCT
GCACAAAAGGCGCAGGGCTTCGTGATCTGTACGCCGGACGTCAAGCTTGGGCCGGGCATCGTCGCGAAGGCGAAGGCTGA
CAATCTGAAAATGATGACGGTGGACGACCGTCTCGTCGACGGCGCGGGTAAGCCGATCGAATCGGTGCCGCACATGGGCA
TCTCGGCATACAACATCGGCAAGCAGGTCGGCGACGGTCTGGCTGCGGAAATCAAGAAGCGTGGCTGGGACATGAAGGAC
GTCGGCGCGATCGACGTGACCTACGAACAGCTGCCGACGGCACATGACCGCACGTCGGGCGCCACCGACGCGCTGATTGC
AGCCGGCTTCCCGAAGGCCAACGTGATCGCCGCGCCGCAAGCCAAGACGGACACGGAAAACGCGTTCAACGCAGCGAATA
TCGCGCTGACGAAGAATCCGAACTTCAAGCACTGGATCGCCTACGGCCTGAACGACGAAGCCGTGCTCGGCGCGGTGCGC
GCAGCAGAAGGCCGCGGCTTCAAGGCCGACAACATGATCGGCATCGGCATCGGCGGCTCGGACTCGGCGCTGAACGAGTT
CAAGAAGCCGAACCCGACGGGCTTCTTCGGCACCGTGATCATCAGCCCGAAGCGTCACGGCGAAGAGACGTCTGAACTGA
TGTACGCGTGGATCACGCAAGGCAAGGAGCCGCCGAAGCTCACGCTGACGACGGGCATGCTGGCCACGCGCGACAACGTC
GGCGAAGTGCGTGAAAAGATGGGCCTGGCATCGAAGTAA

Upstream 100 bases:

>100_bases
CGGTGTGAGCCGCCTGACGCGCTGGCCGCGTGTGTCGATGGCGGTACAAGAAGACGCGGCCAATACCTTCTCGTTACTCA
TTAGTCAGGAGACACGCAAC

Downstream 100 bases:

>100_bases
GCGGCATGTGAGGCCAGGGCGCGCGAAGGCTGAGAACGGCGTTCGCGCGCCGTGAAAGACGTAACGAACGAGAAGGAGGC
AAAGTGTCAGCGACGCTGCG

Product: monosaccharide-transporting ATPase

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKRRIFLTLAAAATAVLFNAPVAQAADTVKIGFLVKQPEEPWFQDEWKFAEQAAKDKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALIAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWIAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPNPTGFFGTVIISPKRHGEETSELMYAWITQGKEPPKLTLTTGMLATRDNV
GEVREKMGLASK

Sequences:

>Translated_332_residues
MKRRIFLTLAAAATAVLFNAPVAQAADTVKIGFLVKQPEEPWFQDEWKFAEQAAKDKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALIAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWIAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPNPTGFFGTVIISPKRHGEETSELMYAWITQGKEPPKLTLTTGMLATRDNV
GEVREKMGLASK
>Mature_332_residues
MKRRIFLTLAAAATAVLFNAPVAQAADTVKIGFLVKQPEEPWFQDEWKFAEQAAKDKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADNLKMMTVDDRLVDGAGKPIESVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALIAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWIAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPNPTGFFGTVIISPKRHGEETSELMYAWITQGKEPPKLTLTTGMLATRDNV
GEVREKMGLASK

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=308, Percent_Identity=50.974025974026, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35369; Mature: 35369

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIFLTLAAAATAVLFNAPVAQAADTVKIGFLVKQPEEPWFQDEWKFAEQAAKDKGFT
CCEEEEEEEHHHHHHHHHCCCHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCE
LVKIGAPSGEKVMSAIDNLAAQKAQGFVICTPDVKLGPGIVAKAKADNLKMMTVDDRLVD
EEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEECCCCEEEEEECCHHHC
GAGKPIESVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKDVGAIDVTYEQLPTAHDRTSG
CCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCC
ATDALIAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWIAYGLNDEAVLGAVR
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
AAEGRGFKADNMIGIGIGGSDSALNEFKKPNPTGFFGTVIISPKRHGEETSELMYAWITQ
HCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHC
GKEPPKLTLTTGMLATRDNVGEVREKMGLASK
CCCCCEEEEEECCEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKRRIFLTLAAAATAVLFNAPVAQAADTVKIGFLVKQPEEPWFQDEWKFAEQAAKDKGFT
CCEEEEEEEHHHHHHHHHCCCHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCE
LVKIGAPSGEKVMSAIDNLAAQKAQGFVICTPDVKLGPGIVAKAKADNLKMMTVDDRLVD
EEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEECCCCEEEEEECCHHHC
GAGKPIESVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKDVGAIDVTYEQLPTAHDRTSG
CCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCC
ATDALIAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWIAYGLNDEAVLGAVR
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
AAEGRGFKADNMIGIGIGGSDSALNEFKKPNPTGFFGTVIISPKRHGEETSELMYAWITQ
HCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHC
GKEPPKLTLTTGMLATRDNVGEVREKMGLASK
CCCCCEEEEEECCEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]