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The map label for this gene is rsmE [H]

Identifier: 186477349

GI number: 186477349

Start: 2939870

End: 2940625

Strand: Direct

Name: rsmE [H]

Synonym: Bphy_2601

Alternate gene names: 186477349

Gene position: 2939870-2940625 (Clockwise)

Preceding gene: 186477348

Following gene: 186477353

Centisome position: 84.5

GC content: 65.08

Gene sequence:

>756_bases
ATGAGAACTTTCCTTATGCCTCGCTTCTTCGTCGGTACCCCGTTTAAGACCGACGACATTGTTTCCTTGCCGGATGAAGT
CAACCGGCACGTCCGCGTCCTGCGGCTGCAACCGGGCGACACCGTCAGCCTGTTTAATGGCGACGGCGGCGAGTACGGCG
CTGAAATCGTCGATATGGATCGACATGGAACGATCGTGCGCATCGTCGGATTTCGCGACATCGAATGCGAACCGCCGTAC
CGGCTGACGCTCGCCCAAGGTATCGCGGGTAGCGACAAGATGGACTGGCTGATCGAAAAGGCTACCGAACTGGGCGCGTG
CGAATTCGTGCCGCTCACCACGACTCGCAGCGTCGTGCGGCTCGCCGGCGAGCGCGCGCAACGCCGCCAGGTTCATTGGC
AGCGGATCGTGCAGGCGTCCTGCGAACAGTGCGGCCGCAATCGCCTGCCCCAAGTCATGCCGACGCGCGAACTGGCCACC
TGGCTCAGCACGATGCCGAAGGAGCCTGTCGACGGCGAACTGCGCCTGCTGCTGTCGCCGCGTGCGAGCGTGCCGTTTTC
CGCGCTGCCTGTCGAGCCGCCGAAGGTGCGTGCGCTGGTACTGGTCGGCCCGGAAGGCGGCTTTTCGGCTGCTGAAGAAG
CAGCAGCAGCGGCTCACGGCTTCACGGCTGTCGGCCTCGGCCCACGCGTGCTGCGTACCGAAACGGCCGGCATTGCGGTG
CTGTCCGCACTGGCTGCGCGCTGGGGCGGCTGGTAA

Upstream 100 bases:

>100_bases
CTCAGCGAACCGCAAGATCAGTTCTGGGGCGACCGTTTTGCCCAGATCGAAGATCTCGACGGCTACCGCTGGGCGTTAGC
CTGCCATCTGTCTTGAGTGA

Downstream 100 bases:

>100_bases
GCGGCGCTTCGGCGCTTGCACCACCACAACGAAGCCCGACTGAAATGCAAAAGGCCCGCCTGTTTCCGGCGGGCCTTTCT
TTTTTATTGCAGATCAGACG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRTFLMPRFFVGTPFKTDDIVSLPDEVNRHVRVLRLQPGDTVSLFNGDGGEYGAEIVDMDRHGTIVRIVGFRDIECEPPY
RLTLAQGIAGSDKMDWLIEKATELGACEFVPLTTTRSVVRLAGERAQRRQVHWQRIVQASCEQCGRNRLPQVMPTRELAT
WLSTMPKEPVDGELRLLLSPRASVPFSALPVEPPKVRALVLVGPEGGFSAAEEAAAAAHGFTAVGLGPRVLRTETAGIAV
LSALAARWGGW

Sequences:

>Translated_251_residues
MRTFLMPRFFVGTPFKTDDIVSLPDEVNRHVRVLRLQPGDTVSLFNGDGGEYGAEIVDMDRHGTIVRIVGFRDIECEPPY
RLTLAQGIAGSDKMDWLIEKATELGACEFVPLTTTRSVVRLAGERAQRRQVHWQRIVQASCEQCGRNRLPQVMPTRELAT
WLSTMPKEPVDGELRLLLSPRASVPFSALPVEPPKVRALVLVGPEGGFSAAEEAAAAAHGFTAVGLGPRVLRTETAGIAV
LSALAARWGGW
>Mature_251_residues
MRTFLMPRFFVGTPFKTDDIVSLPDEVNRHVRVLRLQPGDTVSLFNGDGGEYGAEIVDMDRHGTIVRIVGFRDIECEPPY
RLTLAQGIAGSDKMDWLIEKATELGACEFVPLTTTRSVVRLAGERAQRRQVHWQRIVQASCEQCGRNRLPQVMPTRELAT
WLSTMPKEPVDGELRLLLSPRASVPFSALPVEPPKVRALVLVGPEGGFSAAEEAAAAAHGFTAVGLGPRVLRTETAGIAV
LSALAARWGGW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=245, Percent_Identity=37.1428571428571, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27482; Mature: 27482

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFLMPRFFVGTPFKTDDIVSLPDEVNRHVRVLRLQPGDTVSLFNGDGGEYGAEIVDMD
CCCCCCCHHHCCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEC
RHGTIVRIVGFRDIECEPPYRLTLAQGIAGSDKMDWLIEKATELGACEFVPLTTTRSVVR
CCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHH
LAGERAQRRQVHWQRIVQASCEQCGRNRLPQVMPTRELATWLSTMPKEPVDGELRLLLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECC
RASVPFSALPVEPPKVRALVLVGPEGGFSAAEEAAAAAHGFTAVGLGPRVLRTETAGIAV
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHEEECHHHHHH
LSALAARWGGW
HHHHHHHCCCC
>Mature Secondary Structure
MRTFLMPRFFVGTPFKTDDIVSLPDEVNRHVRVLRLQPGDTVSLFNGDGGEYGAEIVDMD
CCCCCCCHHHCCCCCCCCCCCCCCHHHCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEC
RHGTIVRIVGFRDIECEPPYRLTLAQGIAGSDKMDWLIEKATELGACEFVPLTTTRSVVR
CCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHH
LAGERAQRRQVHWQRIVQASCEQCGRNRLPQVMPTRELATWLSTMPKEPVDGELRLLLSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECC
RASVPFSALPVEPPKVRALVLVGPEGGFSAAEEAAAAAHGFTAVGLGPRVLRTETAGIAV
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCHHEEECHHHHHH
LSALAARWGGW
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]