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The map label for this gene is holA [H]

Identifier: 186477334

GI number: 186477334

Start: 2923129

End: 2924256

Strand: Reverse

Name: holA [H]

Synonym: Bphy_2586

Alternate gene names: 186477334

Gene position: 2924256-2923129 (Counterclockwise)

Preceding gene: 186477335

Following gene: 186477333

Centisome position: 84.05

GC content: 67.64

Gene sequence:

>1128_bases
ATGCAACTGCGTCTTGACGCGCTCGAAGCGCATCTCGCGAAAGGTCTCGCCGGTCTGTACATCGTGTACGGCGACGAGCA
TCTGCTCGCGCAGGAAGCGTGCGACCGCATCCGCGCGGCGGCGCGTGCGGGCGGCTTCACGGATCGCACGGTGTTCACGG
TGGAGCGCGGCTTCGACTGGAGCTCGCTGCTCGGCGCGAGCCAGTCGATGTCGCTGTTTGGCGACCGGCAACTTGTCGAG
TTGCGCATTCCGTCAGGCAAGCCCGGCAAGGAAGGCGCCGACGCGCTCAAGACTCTGGCCGCAGCCGCCAACCCCGACGT
GCTGACGCTCGTCACGCTGCCGCGTCTCGACGCGGCGACGCAAAAGGCCGCGTGGTTCACTGCGCTGGCCGACGCAGGCG
TCGCGCTGAAGATAGATCCCGTCGAGCGTGCGCAATTGCCTGGGTGGATCAGTCAGCGCCTCGCGTCGCAGAATCAGCGA
GTTGCGCCAGGTGAAGACGGCAAGCGCGCGTTGCAGTTCATCGCGGAGCGCGTCGAAGGCAATCTGCTCGCGGCGCATCA
GGAGATCCAGAAGCTCGGGCTGCTGTATCCCTCTGGCGTATTGACGTTCGAACAGGTTCACGACGCGGTGCTGAACGTCG
CGCGCTACGACGTGTTCAAGCTCAACGAAGCGATGCTCGCGGGCGACGTCGGCCGTCTGTCGCGCATGATTGACGGTCTG
AAGGGCGAAGGCGAAGCAGCCGTGCTGGTGCTGTGGGCCGTCGTCGAAGAAATCCGCACGCTGCTGCGGATCAAGCGCGG
CGTCGATGCGGGCAAGCCGCTTGCCATGCTTTTGCGTGAAAACCGCGTGTGGGGGCCGCGTGAACGGCTCGTCGGTCCGG
CGCTGTCGCGTGTGACGGAAGCCGCGCTGGAACGGGCGCTCTCGCTCGCGGCCAGACTCGACCGGCAGGTCAAGGGGCTG
TCGGGCGGCACGCCAGGCGATCGTCGCAATGACCCGCCGCCCGATCCGTGGGCGGGACTGTTCGAACTTGCGTTGACGGT
CGCCGCGGCGCCGGCGCCGAAGACGCACGCGGCGCCGGTCGGACGGGCGCCGTCAGCGCGAAGCGCGCCGTTACGCCGTC
CGTCCTAG

Upstream 100 bases:

>100_bases
GGGACAGGAAGTGCCGGGCATCTCGCCGCGCGCGCCGCTGCCGCCGCCACCGCTTTGATCCACCGACTCGACCCGCTTTC
ACTCTCAGTCACCCGCCGCC

Downstream 100 bases:

>100_bases
GCTGTCGCATCCGTTGCGGTTCGCGCGCCGGAACGCGCGGGTCCTCTGGCAGGCGGCGCGCGCAACCGGCCGGTCACGTC
CCGATGCGGGGCTCGGCGGT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQLRLDALEAHLAKGLAGLYIVYGDEHLLAQEACDRIRAAARAGGFTDRTVFTVERGFDWSSLLGASQSMSLFGDRQLVE
LRIPSGKPGKEGADALKTLAAAANPDVLTLVTLPRLDAATQKAAWFTALADAGVALKIDPVERAQLPGWISQRLASQNQR
VAPGEDGKRALQFIAERVEGNLLAAHQEIQKLGLLYPSGVLTFEQVHDAVLNVARYDVFKLNEAMLAGDVGRLSRMIDGL
KGEGEAAVLVLWAVVEEIRTLLRIKRGVDAGKPLAMLLRENRVWGPRERLVGPALSRVTEAALERALSLAARLDRQVKGL
SGGTPGDRRNDPPPDPWAGLFELALTVAAAPAPKTHAAPVGRAPSARSAPLRRPS

Sequences:

>Translated_375_residues
MQLRLDALEAHLAKGLAGLYIVYGDEHLLAQEACDRIRAAARAGGFTDRTVFTVERGFDWSSLLGASQSMSLFGDRQLVE
LRIPSGKPGKEGADALKTLAAAANPDVLTLVTLPRLDAATQKAAWFTALADAGVALKIDPVERAQLPGWISQRLASQNQR
VAPGEDGKRALQFIAERVEGNLLAAHQEIQKLGLLYPSGVLTFEQVHDAVLNVARYDVFKLNEAMLAGDVGRLSRMIDGL
KGEGEAAVLVLWAVVEEIRTLLRIKRGVDAGKPLAMLLRENRVWGPRERLVGPALSRVTEAALERALSLAARLDRQVKGL
SGGTPGDRRNDPPPDPWAGLFELALTVAAAPAPKTHAAPVGRAPSARSAPLRRPS
>Mature_375_residues
MQLRLDALEAHLAKGLAGLYIVYGDEHLLAQEACDRIRAAARAGGFTDRTVFTVERGFDWSSLLGASQSMSLFGDRQLVE
LRIPSGKPGKEGADALKTLAAAANPDVLTLVTLPRLDAATQKAAWFTALADAGVALKIDPVERAQLPGWISQRLASQNQR
VAPGEDGKRALQFIAERVEGNLLAAHQEIQKLGLLYPSGVLTFEQVHDAVLNVARYDVFKLNEAMLAGDVGRLSRMIDGL
KGEGEAAVLVLWAVVEEIRTLLRIKRGVDAGKPLAMLLRENRVWGPRERLVGPALSRVTEAALERALSLAARLDRQVKGL
SGGTPGDRRNDPPPDPWAGLFELALTVAAAPAPKTHAAPVGRAPSARSAPLRRPS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=346, Percent_Identity=32.3699421965318, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40269; Mature: 40269

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRLDALEAHLAKGLAGLYIVYGDEHLLAQEACDRIRAAARAGGFTDRTVFTVERGFDW
CCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
SSLLGASQSMSLFGDRQLVELRIPSGKPGKEGADALKTLAAAANPDVLTLVTLPRLDAAT
HHHHCCCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
QKAAWFTALADAGVALKIDPVERAQLPGWISQRLASQNQRVAPGEDGKRALQFIAERVEG
HHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
NLLAAHQEIQKLGLLYPSGVLTFEQVHDAVLNVARYDVFKLNEAMLAGDVGRLSRMIDGL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
KGEGEAAVLVLWAVVEEIRTLLRIKRGVDAGKPLAMLLRENRVWGPRERLVGPALSRVTE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALERALSLAARLDRQVKGLSGGTPGDRRNDPPPDPWAGLFELALTVAAAPAPKTHAAPV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GRAPSARSAPLRRPS
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLRLDALEAHLAKGLAGLYIVYGDEHLLAQEACDRIRAAARAGGFTDRTVFTVERGFDW
CCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
SSLLGASQSMSLFGDRQLVELRIPSGKPGKEGADALKTLAAAANPDVLTLVTLPRLDAAT
HHHHCCCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
QKAAWFTALADAGVALKIDPVERAQLPGWISQRLASQNQRVAPGEDGKRALQFIAERVEG
HHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
NLLAAHQEIQKLGLLYPSGVLTFEQVHDAVLNVARYDVFKLNEAMLAGDVGRLSRMIDGL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
KGEGEAAVLVLWAVVEEIRTLLRIKRGVDAGKPLAMLLRENRVWGPRERLVGPALSRVTE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALERALSLAARLDRQVKGLSGGTPGDRRNDPPPDPWAGLFELALTVAAAPAPKTHAAPV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GRAPSARSAPLRRPS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]