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The map label for this gene is leuE [H]

Identifier: 186477303

GI number: 186477303

Start: 2877110

End: 2877742

Strand: Reverse

Name: leuE [H]

Synonym: Bphy_2555

Alternate gene names: 186477303

Gene position: 2877742-2877110 (Counterclockwise)

Preceding gene: 186477306

Following gene: 186477296

Centisome position: 82.71

GC content: 62.24

Gene sequence:

>633_bases
ATGCTCAGTCTCACCACCTTCGCGCTGTTTTCAGGCGCTTGTGTTGCGCTCACCGCCACACCCGGCCCGGATATGTTGCT
GATTGCCTCCCGCAGCGTGAGCCAAGGCAGGACGGCGGGTTTCGCGTCGCTTGCCGGGATTCTGGTTGGTACGTATTGTC
ACGCTCTTGCAGCGGCGTTCGGCCTGTCACAGCTCTTTCTCGCCGCCCCGATGGCCTACGACATCGTGCGCTTCAGCGGC
GCTGCCTGCCTGCTCTATCTCGCTTGGAAGACGTTCCGCTCGCAGGGCACGGTGCTGGCGCCGAACGCGTCGATGCGGCG
CTTTCCCATTGACCGGATTTTCAGGCAAGGGTTGTTCACGAACCTGCTCAATCCGAAGGTAGCGCTGTTCGTGCTCGCAC
TGTTTCCGCAGTTTGTGAAACCGCAGGCAGGGTCGGTTGTCGTGCAGATCATGCTGCTTGCGACCGTGCTCAACGTGATC
GGCTTCGTGGTGAATGGCAGCGTGATTTTGCTTGCCAGCAGGCTGAGCGTGAAACTGACTGGCGGCGAACGCCCGCCGCG
CTGGCCGCAATATTTGCTCGGCTCGGTGTTCGCCGGCCTGGCGTGTCGGCTGGCACTGGCGGGACAACGCTGA

Upstream 100 bases:

>100_bases
TCGCTTCTGACAAAGTGGGAGAGCGGCTGATTGACTCGGCCTCCCTTGCTTCCTACACTCGGATCCCTTTTGGACGTTCC
ATAACCCAAAGGGGTCTCGG

Downstream 100 bases:

>100_bases
CGGCAAAGGCGCCTAACCTACCAACCGCCCGCGACGCACTCGCAAGCCCTTTTTCGCGAGTCCCGGTGCAATACCACCCA
GTGTGCTCAATGTGCCGCCG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MLSLTTFALFSGACVALTATPGPDMLLIASRSVSQGRTAGFASLAGILVGTYCHALAAAFGLSQLFLAAPMAYDIVRFSG
AACLLYLAWKTFRSQGTVLAPNASMRRFPIDRIFRQGLFTNLLNPKVALFVLALFPQFVKPQAGSVVVQIMLLATVLNVI
GFVVNGSVILLASRLSVKLTGGERPPRWPQYLLGSVFAGLACRLALAGQR

Sequences:

>Translated_210_residues
MLSLTTFALFSGACVALTATPGPDMLLIASRSVSQGRTAGFASLAGILVGTYCHALAAAFGLSQLFLAAPMAYDIVRFSG
AACLLYLAWKTFRSQGTVLAPNASMRRFPIDRIFRQGLFTNLLNPKVALFVLALFPQFVKPQAGSVVVQIMLLATVLNVI
GFVVNGSVILLASRLSVKLTGGERPPRWPQYLLGSVFAGLACRLALAGQR
>Mature_210_residues
MLSLTTFALFSGACVALTATPGPDMLLIASRSVSQGRTAGFASLAGILVGTYCHALAAAFGLSQLFLAAPMAYDIVRFSG
AACLLYLAWKTFRSQGTVLAPNASMRRFPIDRIFRQGLFTNLLNPKVALFVLALFPQFVKPQAGSVVVQIMLLATVLNVI
GFVVNGSVILLASRLSVKLTGGERPPRWPQYLLGSVFAGLACRLALAGQR

Specific function: Exporter of leucine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=175, Percent_Identity=29.7142857142857, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1788099, Length=185, Percent_Identity=28.1081081081081, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786522, Length=120, Percent_Identity=31.6666666666667, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22310; Mature: 22310

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLTTFALFSGACVALTATPGPDMLLIASRSVSQGRTAGFASLAGILVGTYCHALAAAF
CCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLSQLFLAAPMAYDIVRFSGAACLLYLAWKTFRSQGTVLAPNASMRRFPIDRIFRQGLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHH
NLLNPKVALFVLALFPQFVKPQAGSVVVQIMLLATVLNVIGFVVNGSVILLASRLSVKLT
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
GGERPPRWPQYLLGSVFAGLACRLALAGQR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSLTTFALFSGACVALTATPGPDMLLIASRSVSQGRTAGFASLAGILVGTYCHALAAAF
CCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLSQLFLAAPMAYDIVRFSGAACLLYLAWKTFRSQGTVLAPNASMRRFPIDRIFRQGLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHH
NLLNPKVALFVLALFPQFVKPQAGSVVVQIMLLATVLNVIGFVVNGSVILLASRLSVKLT
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
GGERPPRWPQYLLGSVFAGLACRLALAGQR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA