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The map label for this gene is pntB [H]

Identifier: 186477289

GI number: 186477289

Start: 2861764

End: 2863215

Strand: Reverse

Name: pntB [H]

Synonym: Bphy_2541

Alternate gene names: 186477289

Gene position: 2863215-2861764 (Counterclockwise)

Preceding gene: 186477290

Following gene: 186477288

Centisome position: 82.3

GC content: 61.16

Gene sequence:

>1452_bases
ATGAGCCTGAACGTCGTTACGCTCCTGTATCTGATTGCGTCGGTGTGCTTCATCCAGGCGCTCAAAGGGCTGTCAAATCC
GAAGACCGCGCGCACGGGTAACGTGTTCGGCATGGTCGGCATGGCGATCGCGATTCTCACTACCGTCGCGCTGATTGCCA
AGCAGGCCGCGTTTCTCGGCTCGAACCTCGGGCTCGGGTTGTCGCTGGTATTCATTGCGTTGGTCGTCGGCGGCGGGATC
GGTGCGTTCGTTGCCGCTCGCGTCGAAATGACGAAGATGCCGGAGCTGGTCGCGGCGATGCACTCGCTGATCGGTATGGC
GGCCGTGTGTATCGCGTATGCCGTCGTGTCCGAACCGGCTGCGTTCGGTCTCGGTCTGGAAGAAGGCATTCAGGGCTTCA
TTCCGTATGGCAACCGGATCGAGCTGTTCATCGGCACCTTCGTCGGCGCGATTACCTTCAGCGGTTCGGTGATCGCGTTC
GGCAAGCTGTCGGGCAAGTACAAGTTCCGGCTGTTCCAGGGCGCGCCTGTCGTCTATGCGGGCCAGCATCTGATTAACCT
GCTGCTCGCGATCGCGATGATCGGCTTCGGCGTGATCTTCTTCCTCACGCAATCGTGGCTGCCGTTCATCATCATGACGC
TGATCGCGTTCGCGCTGGGCGTGCTGATCATCATCCCGATCGGCGGCGCGGACATGCCGGTGGTGGTGTCGATGCTGAAC
TCGTACTCGGGCTGGGCGGCGGCGGGCATCGGCTTCTCGCTGAACAACGCGATGCTGATCATTGCCGGTTCGCTGGTCGG
CTCGTCGGGTGCGATTCTGTCGTACATCATGTGCAAGGCGATGAACCGCTCGTTCTTCAACGTGATTCTGGGCGGCTTCG
GCGGCGAAGCGGGCGCGGCGGCTGCGGCCGGTTCGGGCGAACAGAAGCCGGTGAAGTCGGGTTCGGCGGAAGATGCGTCG
TTCATGCTCGGCAATGCTGAGACCGTGGTGATCGTGCCGGGTTATGGTCTCGCGGTTGCGCGTGCACAGCATGCGCTGAA
GGAACTGACCGATCTGCTGATCGAGAAGGGCGTGGACGTGAAGTACGCGATTCACCCGGTTGCCGGGCGGATGCCGGGTC
ACATGAACGTGCTGCTGGCGGAAGCCGAAGTGCCGTACGAGATCGTGCACGAGATGGAGGACATCAACGGCGAGTTCGGC
CAGGTCGACGTGGTGCTGGTGCTTGGCGCGAACGACGTGGTGAACCCGGCTGCGAAGACCGATCCGAAGTCACCCATTGC
TGGCATGCCGATCATCGAGGCGTACAAGGCCCGCACGGTGATCGTCAACAAGCGGTCGATGGCGGCGGGGTATGCCGGAC
TCGACAACGATCTCTTCTACATGGATAAAACCATGATGGTGTTTGGCGATGCCAAGAAGGTGATCGAGGATATGGTCAAG
GCAGTGGATTGA

Upstream 100 bases:

>100_bases
GGTGAACGTGTTCGGCGGGTTCCTCGTTACGAGGCGAATGCTGGAAATGTTCAAGAAGAAGGAACCGAAGAAGATTGCCG
GTCCGAAGGAGGGTGCGTAA

Downstream 100 bases:

>100_bases
TTTTCGCGTACCGCGGATGCTGGCTTTTTTCGCGCCGGCATCCGCGAATAGCATGTCGCCCAGCGTCAGCTTCCTCGACT
CTGCATTGCAGGCTTGCTCA

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSLNVVTLLYLIASVCFIQALKGLSNPKTARTGNVFGMVGMAIAILTTVALIAKQAAFLGSNLGLGLSLVFIALVVGGGI
GAFVAARVEMTKMPELVAAMHSLIGMAAVCIAYAVVSEPAAFGLGLEEGIQGFIPYGNRIELFIGTFVGAITFSGSVIAF
GKLSGKYKFRLFQGAPVVYAGQHLINLLLAIAMIGFGVIFFLTQSWLPFIIMTLIAFALGVLIIIPIGGADMPVVVSMLN
SYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAGAAAAAGSGEQKPVKSGSAEDAS
FMLGNAETVVIVPGYGLAVARAQHALKELTDLLIEKGVDVKYAIHPVAGRMPGHMNVLLAEAEVPYEIVHEMEDINGEFG
QVDVVLVLGANDVVNPAAKTDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVK
AVD

Sequences:

>Translated_483_residues
MSLNVVTLLYLIASVCFIQALKGLSNPKTARTGNVFGMVGMAIAILTTVALIAKQAAFLGSNLGLGLSLVFIALVVGGGI
GAFVAARVEMTKMPELVAAMHSLIGMAAVCIAYAVVSEPAAFGLGLEEGIQGFIPYGNRIELFIGTFVGAITFSGSVIAF
GKLSGKYKFRLFQGAPVVYAGQHLINLLLAIAMIGFGVIFFLTQSWLPFIIMTLIAFALGVLIIIPIGGADMPVVVSMLN
SYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAGAAAAAGSGEQKPVKSGSAEDAS
FMLGNAETVVIVPGYGLAVARAQHALKELTDLLIEKGVDVKYAIHPVAGRMPGHMNVLLAEAEVPYEIVHEMEDINGEFG
QVDVVLVLGANDVVNPAAKTDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVK
AVD
>Mature_482_residues
SLNVVTLLYLIASVCFIQALKGLSNPKTARTGNVFGMVGMAIAILTTVALIAKQAAFLGSNLGLGLSLVFIALVVGGGIG
AFVAARVEMTKMPELVAAMHSLIGMAAVCIAYAVVSEPAAFGLGLEEGIQGFIPYGNRIELFIGTFVGAITFSGSVIAFG
KLSGKYKFRLFQGAPVVYAGQHLINLLLAIAMIGFGVIFFLTQSWLPFIIMTLIAFALGVLIIIPIGGADMPVVVSMLNS
YSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAGAAAAAGSGEQKPVKSGSAEDASF
MLGNAETVVIVPGYGLAVARAQHALKELTDLLIEKGVDVKYAIHPVAGRMPGHMNVLLAEAEVPYEIVHEMEDINGEFGQ
VDVVLVLGANDVVNPAAKTDPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVKA
VD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=482, Percent_Identity=45.2282157676349, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI122939153, Length=482, Percent_Identity=45.2282157676349, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1787886, Length=487, Percent_Identity=48.870636550308, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17550456, Length=483, Percent_Identity=42.2360248447205, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50406; Mature: 50275

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNVVTLLYLIASVCFIQALKGLSNPKTARTGNVFGMVGMAIAILTTVALIAKQAAFLG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SNLGLGLSLVFIALVVGGGIGAFVAARVEMTKMPELVAAMHSLIGMAAVCIAYAVVSEPA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFGLGLEEGIQGFIPYGNRIELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVVYA
HHCCCHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCEEEH
GQHLINLLLAIAMIGFGVIFFLTQSWLPFIIMTLIAFALGVLIIIPIGGADMPVVVSMLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
SYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAGAA
CCCCCCHHCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
AAAGSGEQKPVKSGSAEDASFMLGNAETVVIVPGYGLAVARAQHALKELTDLLIEKGVDV
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCE
KYAIHPVAGRMPGHMNVLLAEAEVPYEIVHEMEDINGEFGQVDVVLVLGANDVVNPAAKT
EEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
DPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVK
CCCCCCCCCCHHHHHCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEECCHHHHHHHHHH
AVD
HCC
>Mature Secondary Structure 
SLNVVTLLYLIASVCFIQALKGLSNPKTARTGNVFGMVGMAIAILTTVALIAKQAAFLG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SNLGLGLSLVFIALVVGGGIGAFVAARVEMTKMPELVAAMHSLIGMAAVCIAYAVVSEPA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFGLGLEEGIQGFIPYGNRIELFIGTFVGAITFSGSVIAFGKLSGKYKFRLFQGAPVVYA
HHCCCHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCEEEH
GQHLINLLLAIAMIGFGVIFFLTQSWLPFIIMTLIAFALGVLIIIPIGGADMPVVVSMLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
SYSGWAAAGIGFSLNNAMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAGAA
CCCCCCHHCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
AAAGSGEQKPVKSGSAEDASFMLGNAETVVIVPGYGLAVARAQHALKELTDLLIEKGVDV
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCE
KYAIHPVAGRMPGHMNVLLAEAEVPYEIVHEMEDINGEFGQVDVVLVLGANDVVNPAAKT
EEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
DPKSPIAGMPIIEAYKARTVIVNKRSMAAGYAGLDNDLFYMDKTMMVFGDAKKVIEDMVK
CCCCCCCCCCHHHHHCCEEEEEECHHHHCCCCCCCCCEEEECCEEEEECCHHHHHHHHHH
AVD
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]