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The map label for this gene is citH [H]

Identifier: 186477277

GI number: 186477277

Start: 2850902

End: 2852179

Strand: Reverse

Name: citH [H]

Synonym: Bphy_2529

Alternate gene names: 186477277

Gene position: 2852179-2850902 (Counterclockwise)

Preceding gene: 186477278

Following gene: 186477271

Centisome position: 81.98

GC content: 63.77

Gene sequence:

>1278_bases
ATGAGTTCAACGATAGCCGCGAGCACGGCGCAGCGCCGTTCCGATACGTGGCGCACGGTGGTGGCCGCGTCGATCGGCAA
CGCGCTGGAATGGTTCGACCTCGTCGTTTACGGTTTTTTCGCCGTCGTCATTTCGAAGCTGTTCTTTCCGGCGGGCAACG
AGACCGTCTCCCTGCTGCTGACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGGCCGCTCGGTGCGATCATGCTCGGG
GCCTATGCGGACCGGGCAGGGCGCAAGGCTGCGCTGACACTCTCCATTCTGCTGATGATGATGGGCACGCTGATCATCGC
CGTCCTGCCGACGTACCAGTCGATCGGCCTTGCGGCACCGCTGATTCTCGTCGTCGCGCGTCTGATGCAGGGCTTTTCGG
CGGGCGGGGAGTTCGGCAGCGCGACAGCCTTTCTCGCCGAACACGTGCCCGGGCGGCGTGGCTATTTCGCCAGCTGGCAG
ATCGCGAGCCAGGGGCTGACTACCCTGCTGGCCGCACTGTTCGGGGCCATGCTGACGGGGCAGCTATCGCCCGAGCAGCT
GAACGCGTGGGGCTGGCGCGTGCCGTTTTTCTTCGGCCTGTTGATCGGGCCCGTCGCGTGGTACATCCGCACGAAGCTCG
ACGAAACGCCGGAGTTCCTCGCCGCCGAAACGACGCAAACGCCGATTCGCGATACGTTCGCGAGCCAGAAGACGCGGCTT
TTCATCGGCATGGGCATTGTCGTGCTCGGCACCGTGGCGACGTACCTGGTGTTGTTCATGCCGACGTACGCGATCAAGCA
GCTGAAGCTCGCGCCGTCCGTCGCGTTCACGGCGATTTCGCTGGTCGGCGTGATCCAGCTGGTGTGTTCGCCGATCGTCG
GGCATCTGTCGGATCGTCACGGACGCGTGCGCATCATGCTCGTGCCCGCCGTGCTGCTGTTCCTGCTGATCTGCCCGGCC
TTTTCCTATCTCGTCGCGCATCCGACCTTCGGCACGCTGCTGGCGATGCAGATCGTGCTTGGCGTTCTGATGACGAGTTA
CTTCGGGGCGCTGCCCGGCCTGCTGTCGGAAATCTTTCCGGTGCAGACGCGCACGACGGGCATGTCGCTCGCCTATAACA
TCGCGGTGACGATCTTCGGCGGTTTCGGGCCGTTCATCATCGCCTGGCTGATCGACGCGACGGGTTCGAAGATTGCGCCG
GGCTTCTATTTGATGCTCGCGGCCGTGGTGAGTGTGATCGCGCTGATCGCGGCAAGACGCAAGCTCGGTTTCAGGTAA

Upstream 100 bases:

>100_bases
GGATCGGTTGTGCGCGCATCGAATCCGGTGAAAAGATGAAGCGCGGACGATTCTTCGCAATCGTCACGATAAGGACTACC
GCGCGCACTGGAGATCCACA

Downstream 100 bases:

>100_bases
CGCGCTTTTCACCCTGCCGATGATGTAAACGACAAAGGCCGGTTCGATTGAACCGGCCTTTTTCTGGTGCTAACAACGTC
TCTTTCAGACGCTCGCGATC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSSTIAASTAQRRSDTWRTVVAASIGNALEWFDLVVYGFFAVVISKLFFPAGNETVSLLLTLGTFGVSFFMRPLGAIMLG
AYADRAGRKAALTLSILLMMMGTLIIAVLPTYQSIGLAAPLILVVARLMQGFSAGGEFGSATAFLAEHVPGRRGYFASWQ
IASQGLTTLLAALFGAMLTGQLSPEQLNAWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTQTPIRDTFASQKTRL
FIGMGIVVLGTVATYLVLFMPTYAIKQLKLAPSVAFTAISLVGVIQLVCSPIVGHLSDRHGRVRIMLVPAVLLFLLICPA
FSYLVAHPTFGTLLAMQIVLGVLMTSYFGALPGLLSEIFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIDATGSKIAP
GFYLMLAAVVSVIALIAARRKLGFR

Sequences:

>Translated_425_residues
MSSTIAASTAQRRSDTWRTVVAASIGNALEWFDLVVYGFFAVVISKLFFPAGNETVSLLLTLGTFGVSFFMRPLGAIMLG
AYADRAGRKAALTLSILLMMMGTLIIAVLPTYQSIGLAAPLILVVARLMQGFSAGGEFGSATAFLAEHVPGRRGYFASWQ
IASQGLTTLLAALFGAMLTGQLSPEQLNAWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTQTPIRDTFASQKTRL
FIGMGIVVLGTVATYLVLFMPTYAIKQLKLAPSVAFTAISLVGVIQLVCSPIVGHLSDRHGRVRIMLVPAVLLFLLICPA
FSYLVAHPTFGTLLAMQIVLGVLMTSYFGALPGLLSEIFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIDATGSKIAP
GFYLMLAAVVSVIALIAARRKLGFR
>Mature_424_residues
SSTIAASTAQRRSDTWRTVVAASIGNALEWFDLVVYGFFAVVISKLFFPAGNETVSLLLTLGTFGVSFFMRPLGAIMLGA
YADRAGRKAALTLSILLMMMGTLIIAVLPTYQSIGLAAPLILVVARLMQGFSAGGEFGSATAFLAEHVPGRRGYFASWQI
ASQGLTTLLAALFGAMLTGQLSPEQLNAWGWRVPFFFGLLIGPVAWYIRTKLDETPEFLAAETTQTPIRDTFASQKTRLF
IGMGIVVLGTVATYLVLFMPTYAIKQLKLAPSVAFTAISLVGVIQLVCSPIVGHLSDRHGRVRIMLVPAVLLFLLICPAF
SYLVAHPTFGTLLAMQIVLGVLMTSYFGALPGLLSEIFPVQTRTTGMSLAYNIAVTIFGGFGPFIIAWLIDATGSKIAPG
FYLMLAAVVSVIALIAARRKLGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=411, Percent_Identity=33.8199513381995, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=33.7931034482759, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788292, Length=406, Percent_Identity=27.5862068965517, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789941, Length=413, Percent_Identity=33.4140435835351, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87082404, Length=324, Percent_Identity=27.1604938271605, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=29.9065420560748, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45660; Mature: 45529

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCC
>Mature Secondary Structure 
SSTIAASTAQRRSDTWRTVVAASIGNALEWFDLVVYGFFAVVISKLFFPAGNETVSLLL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]