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The map label for this gene is panB

Identifier: 186477243

GI number: 186477243

Start: 2804386

End: 2805201

Strand: Direct

Name: panB

Synonym: Bphy_2494

Alternate gene names: 186477243

Gene position: 2804386-2805201 (Clockwise)

Preceding gene: 186477242

Following gene: 186477249

Centisome position: 80.6

GC content: 63.85

Gene sequence:

>816_bases
ATGACCTATTTGCAGGAGACGAGCCGCAGCGCCGTCACCGTCCCGAAACTGATGGCGATGCGCGAAGCCGGCGAAAAGAT
CGCGATGCTCACCTGCTATGACGCGAGCTTCGCGGCGCTGCTCGATCGCGCGGGTGTCGATTCGCTGCTGATCGGCGACT
CGCTCGGCAACGTGCTGCAAGGACAGAGCACCACGCTGCCCGTCACGCTCGATGAAATCGCCTATCACACAGCCTGCGTC
GCGCGTGCGAAGCCGTCGGCGCTGATCGTCTCCGATATGCCTTTTGGCACCTACGGGACGCCCGCCGATGCGTACGCGAA
CGCCGTCAAACTGATGCAGTCGGGCGCGCAGATGATCAAGCTCGAAGGAGGCGAATGGCTCGCGGATACGGTGCGCTTTC
TGGTCGAGCGCTCAGTGCCCGTGTGCGCGCACGTGGGGCTCACGCCGCAATCGGTACACGCGTTCGGCGGCTTCAAGGTG
CAGGGCAAGACGGAATCGGGCGCGGCCCAACTGCTACGCGATTCGCGCGCGATGCAGGACGCGGGCGCACAACTGCTGGT
GATGGAAGCGATGCCGACACAGCTGGCCGCCGAAGTGACGAAGCAACTGCGTATTCCGACGATCGGCATTGGCGCAGGTG
TCGATTGCTCAGGTCAGGTGCTGGTGCTGCACGACATGCTGGGCATTTTTCCCGGCAAGCGTCCGCGCTTCGTGAAGGAT
TTCATGCAGGGACAGCCGAGTATTCTCGCCGCCGTGGAAGCCTATGTGCGGGCCGTCAAGGACGGCACCTTCCCGGGGCC
GGAGCACACGTTCTGA

Upstream 100 bases:

>100_bases
ACGCATCGAAACGATGCGCGGGCGCAAGGAATTCTTTGTCAAAGGCGGCACGCTATAGCGTCCGGCTGCAGCGTGCGGCC
GTATTACCAACGGACTTCCC

Downstream 100 bases:

>100_bases
TTCAAGCCGGGCGGCGCTTTCGAGCGCCGCTTTTCAGGACCGGGCGCCTGCTTCAGCGGCATCACGGTTGATGCGAGCCT
TTCCCCGCTGCCGGCTCAAT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTYLQETSRSAVTVPKLMAMREAGEKIAMLTCYDASFAALLDRAGVDSLLIGDSLGNVLQGQSTTLPVTLDEIAYHTACV
ARAKPSALIVSDMPFGTYGTPADAYANAVKLMQSGAQMIKLEGGEWLADTVRFLVERSVPVCAHVGLTPQSVHAFGGFKV
QGKTESGAAQLLRDSRAMQDAGAQLLVMEAMPTQLAAEVTKQLRIPTIGIGAGVDCSGQVLVLHDMLGIFPGKRPRFVKD
FMQGQPSILAAVEAYVRAVKDGTFPGPEHTF

Sequences:

>Translated_271_residues
MTYLQETSRSAVTVPKLMAMREAGEKIAMLTCYDASFAALLDRAGVDSLLIGDSLGNVLQGQSTTLPVTLDEIAYHTACV
ARAKPSALIVSDMPFGTYGTPADAYANAVKLMQSGAQMIKLEGGEWLADTVRFLVERSVPVCAHVGLTPQSVHAFGGFKV
QGKTESGAAQLLRDSRAMQDAGAQLLVMEAMPTQLAAEVTKQLRIPTIGIGAGVDCSGQVLVLHDMLGIFPGKRPRFVKD
FMQGQPSILAAVEAYVRAVKDGTFPGPEHTF
>Mature_270_residues
TYLQETSRSAVTVPKLMAMREAGEKIAMLTCYDASFAALLDRAGVDSLLIGDSLGNVLQGQSTTLPVTLDEIAYHTACVA
RAKPSALIVSDMPFGTYGTPADAYANAVKLMQSGAQMIKLEGGEWLADTVRFLVERSVPVCAHVGLTPQSVHAFGGFKVQ
GKTESGAAQLLRDSRAMQDAGAQLLVMEAMPTQLAAEVTKQLRIPTIGIGAGVDCSGQVLVLHDMLGIFPGKRPRFVKDF
MQGQPSILAAVEAYVRAVKDGTFPGPEHTF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=259, Percent_Identity=49.8069498069498, Blast_Score=276, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6319653, Length=278, Percent_Identity=37.0503597122302, Blast_Score=178, Evalue=8e-46,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BURP8 (B2JGD9)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858713.1
- ProteinModelPortal:   B2JGD9
- SMR:   B2JGD9
- GeneID:   6243978
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2494
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28818; Mature: 28687

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 92-92 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLQETSRSAVTVPKLMAMREAGEKIAMLTCYDASFAALLDRAGVDSLLIGDSLGNVLQ
CCCHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCEEECHHHHHHHC
GQSTTLPVTLDEIAYHTACVARAKPSALIVSDMPFGTYGTPADAYANAVKLMQSGAQMIK
CCCCEECEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
LEGGEWLADTVRFLVERSVPVCAHVGLTPQSVHAFGGFKVQGKTESGAAQLLRDSRAMQD
ECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLLVMEAMPTQLAAEVTKQLRIPTIGIGAGVDCSGQVLVLHDMLGIFPGKRPRFVKD
CCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHH
FMQGQPSILAAVEAYVRAVKDGTFPGPEHTF
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TYLQETSRSAVTVPKLMAMREAGEKIAMLTCYDASFAALLDRAGVDSLLIGDSLGNVLQ
CCHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCEEECHHHHHHHC
GQSTTLPVTLDEIAYHTACVARAKPSALIVSDMPFGTYGTPADAYANAVKLMQSGAQMIK
CCCCEECEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
LEGGEWLADTVRFLVERSVPVCAHVGLTPQSVHAFGGFKVQGKTESGAAQLLRDSRAMQD
ECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQLLVMEAMPTQLAAEVTKQLRIPTIGIGAGVDCSGQVLVLHDMLGIFPGKRPRFVKD
CCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHH
FMQGQPSILAAVEAYVRAVKDGTFPGPEHTF
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA