BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is rfbB [H]

Identifier: 186477064

GI number: 186477064

Start: 2597585

End: 2598937

Strand: Reverse

Name: rfbB [H]

Synonym: Bphy_2313

Alternate gene names: 186477064

Gene position: 2598937-2597585 (Counterclockwise)

Preceding gene: 186477065

Following gene: 186477063

Centisome position: 74.7

GC content: 59.05

Gene sequence:

>1353_bases
ATGTCCTCTGACGATATCGCTATCGGCGTACACCATCTCGCCAAACGCTATCAGATCTACGAGACGCCGCGCGACCGGCT
GAAGCAGTTCGTGCTGCCACGCGTTCAGCGGCTGGTTGGCGGGCAGCCGAAGCACTATTTCAGTGAGTTCTGGGCGCTCA
ATGACGTGTCCTTCGAGGTGAAGAAGGGCGAGACGGTCGGGATCATCGGTCGCAACGGGAGCGGCAAGTCCACCCTGCTG
CAGATGATCTGCGGCACCTTGAGCCCGACGCAGGGGCATGTCGAGGTGAACGGCCGGGTGGCTGCGTTGCTCGAACTGGG
CGCGGGCTTCAACCCGGAGTTCACCGGGCGCGAGAACATCTTCATGAACGGGACGCTGCTTGGCCTCAGCAAACAGGAGA
TCGAAGCGCGCTACGACGATATCGTCGCGTTCGCCGACGTCGGCATGTTCATCGATCAGCCCGTCAAGATGTATTCGTCC
GGGATGTACGTTCGAGTCGCGTTTGCGATTGCGGCGAGCATGGACCCGAGCATTCTGATCGTCGACGAGGCGCTGGCCGT
CGGCGATCTCGCGTTCCAGGCCAAGTGCATGGTCCGGCTTCGCGAGTTGATGGACCGCGGCACGACGGTGCTGTTTGTGA
GCCATGATATGAGCAGTGTGCGCAATATCTGTAGCAAGGTGCTCTGGCTGAAGCAGGGGCGCGCGGTTGCGTATGGCGAT
CCGAAGGAGGTGGTCGGGGCTTACGTCAGTGAGATGAACCTCGACATCAACCGGATTACGACAAAGAAGGTGTCCGAAGC
CGAGCCGCAGCCGTTGCCGGGTCCGGTTGAGCAAGTCGTGCCTCACGAGCCGGTGGCGTTCCTCGACGGCCATAGTCGCT
ACGGTGACGGTCGCGCAACCATTCTGGACGTCACACTGATGGATCAGGAAGGACATGGAACGGAATTGCTCGAGTTGCAC
GAGCCGTACAAGCTGAGAGTGATCCTCAGGGCGAACACTGACATCGAGAACCCGGCCATTGGCTATTCGCTGCGCGACTT
CAAGGGGAATCAGATCGTGGGGGCGATGAACTCCAACTTTCCCCACGTCAACATGCCGTCGTTCGAAGCGGGTAAGACTT
ATTGCGTCGATATAACCGGGGTCAATATGCTGGCTCAAGGTGGATATACCGTGAATGTTGGCGTCGAGAACATCGTTCAG
CAGAACAAGGTGCATCAGTATCTGGACGTACTCGAGAACGCGCGGGTATTCCAGAGCACGTTCGGCTCGCAGGCGGAAAA
CATTTTCCCGGCGATGGTCTGGCAGGACGTCGACTTCGAAATCAGGGAAATCAGTGCGGGCGTTGCGGCGTAA

Upstream 100 bases:

>100_bases
CTGCCGAACTGGCTGGGGCTGGCCGTCTACCTGATCGCGGCGTGTGTCGTCGCCTGGGCTGGCTATGCGTGGTTCCAGAA
GACAAGAAAGGGGTTCGCCG

Downstream 100 bases:

>100_bases
AGCGAATGTGTCGTTGGTGGAGGAATGAGCATGGCGTCACCCAACCGGTTTGAACATATCGGCTTCGACGACTTTCGGCG
CTTCGCGCAGGACGAGTCGA

Product: ABC transporter-like protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSSDDIAIGVHHLAKRYQIYETPRDRLKQFVLPRVQRLVGGQPKHYFSEFWALNDVSFEVKKGETVGIIGRNGSGKSTLL
QMICGTLSPTQGHVEVNGRVAALLELGAGFNPEFTGRENIFMNGTLLGLSKQEIEARYDDIVAFADVGMFIDQPVKMYSS
GMYVRVAFAIAASMDPSILIVDEALAVGDLAFQAKCMVRLRELMDRGTTVLFVSHDMSSVRNICSKVLWLKQGRAVAYGD
PKEVVGAYVSEMNLDINRITTKKVSEAEPQPLPGPVEQVVPHEPVAFLDGHSRYGDGRATILDVTLMDQEGHGTELLELH
EPYKLRVILRANTDIENPAIGYSLRDFKGNQIVGAMNSNFPHVNMPSFEAGKTYCVDITGVNMLAQGGYTVNVGVENIVQ
QNKVHQYLDVLENARVFQSTFGSQAENIFPAMVWQDVDFEIREISAGVAA

Sequences:

>Translated_450_residues
MSSDDIAIGVHHLAKRYQIYETPRDRLKQFVLPRVQRLVGGQPKHYFSEFWALNDVSFEVKKGETVGIIGRNGSGKSTLL
QMICGTLSPTQGHVEVNGRVAALLELGAGFNPEFTGRENIFMNGTLLGLSKQEIEARYDDIVAFADVGMFIDQPVKMYSS
GMYVRVAFAIAASMDPSILIVDEALAVGDLAFQAKCMVRLRELMDRGTTVLFVSHDMSSVRNICSKVLWLKQGRAVAYGD
PKEVVGAYVSEMNLDINRITTKKVSEAEPQPLPGPVEQVVPHEPVAFLDGHSRYGDGRATILDVTLMDQEGHGTELLELH
EPYKLRVILRANTDIENPAIGYSLRDFKGNQIVGAMNSNFPHVNMPSFEAGKTYCVDITGVNMLAQGGYTVNVGVENIVQ
QNKVHQYLDVLENARVFQSTFGSQAENIFPAMVWQDVDFEIREISAGVAA
>Mature_449_residues
SSDDIAIGVHHLAKRYQIYETPRDRLKQFVLPRVQRLVGGQPKHYFSEFWALNDVSFEVKKGETVGIIGRNGSGKSTLLQ
MICGTLSPTQGHVEVNGRVAALLELGAGFNPEFTGRENIFMNGTLLGLSKQEIEARYDDIVAFADVGMFIDQPVKMYSSG
MYVRVAFAIAASMDPSILIVDEALAVGDLAFQAKCMVRLRELMDRGTTVLFVSHDMSSVRNICSKVLWLKQGRAVAYGDP
KEVVGAYVSEMNLDINRITTKKVSEAEPQPLPGPVEQVVPHEPVAFLDGHSRYGDGRATILDVTLMDQEGHGTELLELHE
PYKLRVILRANTDIENPAIGYSLRDFKGNQIVGAMNSNFPHVNMPSFEAGKTYCVDITGVNMLAQGGYTVNVGVENIVQQ
NKVHQYLDVLENARVFQSTFGSQAENIFPAMVWQDVDFEIREISAGVAA

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=223, Percent_Identity=25.5605381165919, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI150417984, Length=231, Percent_Identity=24.2424242424242, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI153792144, Length=222, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI6005701, Length=228, Percent_Identity=27.1929824561404, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI27477115, Length=256, Percent_Identity=26.5625, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27436953, Length=290, Percent_Identity=24.1379310344828, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI116734710, Length=216, Percent_Identity=25.462962962963, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI27881501, Length=231, Percent_Identity=20.7792207792208, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI30795238, Length=231, Percent_Identity=20.7792207792208, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI45446740, Length=204, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI47078218, Length=204, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI105990541, Length=242, Percent_Identity=25.2066115702479, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI157502201, Length=247, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI157502203, Length=247, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI27262624, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI89111135, Length=204, Percent_Identity=27.4509803921569, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789891, Length=238, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87081791, Length=204, Percent_Identity=25.9803921568627, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081709, Length=209, Percent_Identity=25.8373205741627, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790467, Length=255, Percent_Identity=26.2745098039216, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786803, Length=234, Percent_Identity=27.3504273504274, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788761, Length=235, Percent_Identity=26.3829787234043, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=25.6637168141593, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788210, Length=208, Percent_Identity=29.8076923076923, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786398, Length=214, Percent_Identity=28.9719626168224, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994883, Length=237, Percent_Identity=28.6919831223629, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787089, Length=214, Percent_Identity=26.1682242990654, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082267, Length=212, Percent_Identity=25.9433962264151, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994997, Length=216, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786319, Length=289, Percent_Identity=23.8754325259516, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788225, Length=212, Percent_Identity=29.7169811320755, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081782, Length=212, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787029, Length=216, Percent_Identity=27.3148148148148, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789032, Length=272, Percent_Identity=24.6323529411765, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI48994943, Length=214, Percent_Identity=24.7663551401869, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081834, Length=205, Percent_Identity=32.6829268292683, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693107, Length=229, Percent_Identity=27.9475982532751, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788165, Length=212, Percent_Identity=30.6603773584906, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787712, Length=209, Percent_Identity=24.8803827751196, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787370, Length=218, Percent_Identity=23.394495412844, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789593, Length=207, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788644, Length=232, Percent_Identity=30.1724137931034, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790525, Length=216, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788450, Length=206, Percent_Identity=26.6990291262136, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788897, Length=209, Percent_Identity=25.8373205741627, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787041, Length=223, Percent_Identity=26.9058295964126, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789873, Length=210, Percent_Identity=29.0476190476191, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787164, Length=248, Percent_Identity=27.0161290322581, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786703, Length=215, Percent_Identity=29.3023255813954, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786981, Length=195, Percent_Identity=28.2051282051282, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786872, Length=222, Percent_Identity=28.3783783783784, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=253, Percent_Identity=26.4822134387352, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533608, Length=225, Percent_Identity=24, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=216, Percent_Identity=25.462962962963, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=216, Percent_Identity=25.462962962963, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=216, Percent_Identity=25.462962962963, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=212, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=210, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=238, Percent_Identity=25.6302521008403, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24666092, Length=230, Percent_Identity=27.3913043478261, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24643674, Length=300, Percent_Identity=25.3333333333333, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648314, Length=224, Percent_Identity=27.2321428571429, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007184, Length=194, Percent_Identity=23.1958762886598, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221500365, Length=194, Percent_Identity=23.1958762886598, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28573571, Length=205, Percent_Identity=22.9268292682927, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28573573, Length=205, Percent_Identity=22.9268292682927, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221512771, Length=202, Percent_Identity=25.2475247524752, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650853, Length=228, Percent_Identity=27.6315789473684, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650855, Length=228, Percent_Identity=27.6315789473684, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281362751, Length=228, Percent_Identity=27.6315789473684, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550390, Length=239, Percent_Identity=24.2677824267782, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI161077321, Length=241, Percent_Identity=28.2157676348548, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 49693; Mature: 49562

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDDIAIGVHHLAKRYQIYETPRDRLKQFVLPRVQRLVGGQPKHYFSEFWALNDVSFEV
CCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
KKGETVGIIGRNGSGKSTLLQMICGTLSPTQGHVEVNGRVAALLELGAGFNPEFTGRENI
ECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCE
FMNGTLLGLSKQEIEARYDDIVAFADVGMFIDQPVKMYSSGMYVRVAFAIAASMDPSILI
EECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEHHHCCCCCEEE
VDEALAVGDLAFQAKCMVRLRELMDRGTTVLFVSHDMSSVRNICSKVLWLKQGRAVAYGD
EECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECC
PKEVVGAYVSEMNLDINRITTKKVSEAEPQPLPGPVEQVVPHEPVAFLDGHSRYGDGRAT
HHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCEE
ILDVTLMDQEGHGTELLELHEPYKLRVILRANTDIENPAIGYSLRDFKGNQIVGAMNSNF
EEEEEEECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEECCCCC
PHVNMPSFEAGKTYCVDITGVNMLAQGGYTVNVGVENIVQQNKVHQYLDVLENARVFQST
CCCCCCCCCCCCEEEEEEECCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHH
FGSQAENIFPAMVWQDVDFEIREISAGVAA
HCCCHHHCCHHHEECCCCCEEEECCCCCCC
>Mature Secondary Structure 
SSDDIAIGVHHLAKRYQIYETPRDRLKQFVLPRVQRLVGGQPKHYFSEFWALNDVSFEV
CCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
KKGETVGIIGRNGSGKSTLLQMICGTLSPTQGHVEVNGRVAALLELGAGFNPEFTGRENI
ECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCE
FMNGTLLGLSKQEIEARYDDIVAFADVGMFIDQPVKMYSSGMYVRVAFAIAASMDPSILI
EECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEHHHCCCCCEEE
VDEALAVGDLAFQAKCMVRLRELMDRGTTVLFVSHDMSSVRNICSKVLWLKQGRAVAYGD
EECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECC
PKEVVGAYVSEMNLDINRITTKKVSEAEPQPLPGPVEQVVPHEPVAFLDGHSRYGDGRAT
HHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCEE
ILDVTLMDQEGHGTELLELHEPYKLRVILRANTDIENPAIGYSLRDFKGNQIVGAMNSNF
EEEEEEECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEECCCCC
PHVNMPSFEAGKTYCVDITGVNMLAQGGYTVNVGVENIVQQNKVHQYLDVLENARVFQST
CCCCCCCCCCCCEEEEEEECCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHH
FGSQAENIFPAMVWQDVDFEIREISAGVAA
HCCCHHHCCHHHEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7533882 [H]