BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is braF [H]

Identifier: 186477005

GI number: 186477005

Start: 2528455

End: 2529318

Strand: Direct

Name: braF [H]

Synonym: Bphy_2254

Alternate gene names: 186477005

Gene position: 2528455-2529318 (Clockwise)

Preceding gene: 186477004

Following gene: 186477006

Centisome position: 72.67

GC content: 61.23

Gene sequence:

>864_bases
ATGAACGAAAACCCGATGGTTCCCGATCTTGCTTTACCCGAAGAGCACGATGGCCGCGCGCTGTCCGGCGTCGCAACGAT
GGGCCGCGTCGTCACGCCTGGCGAGATCGACGTATCGCACGGGGCGATTCTGTATCTGGAAGACGTGACCGTTTCATTCG
ATGGCTTTCGCGCGTTGAATGCATTGTCGCTGTCGATCGATGTGGGCGAACTGCGCTGCATCATCGGGCCGAACGGCGCG
GGCAAGACGACGATGATGGACGTAATCACGGGGAAGACCGCGCCTGATTCGGGCAAGGTGTTTCTGGGCCAGACGATCGA
CCTGACGCGCATGAACGAGCCGTCGATTGCCCGTGCAGGCATAGGCCGCAAGTTTCAGAAGCCGACCGTGTTCGAGCAGC
ATCCCGTGTGGGAGAACCTCGAGCTCGCGATGAAGACGGACAAGGGCTGGATGGCTTCGCTGCGAGCCCGGCTGGACCGT
TCGGCGCAGGCGCGGATCGAAGAGACCCTGTCCTTGATTCGCCTCGAAGGCGAGGCGTTGCGGCCTGCCGGCGAGTTGTC
GCATGGACAGAAGCAGCGACTCGAGATCGGGATGTTGCTGATGCAGCAACCTGCACTTGTGTTGCTCGATGAGCCTGCAG
CCGGCATGACGGATGATGAGACGATGGAGTTGGCTTCATTGCTTAACCGGCTTCGCGGGTCTTGCTCGATGATGGTCGTC
GAGCATGATATGGAGTTTGTGGCCGCTCTGGCGGGTGAAACCGGCAAGGTGACGGTGATGGCCGAGGGCCGGGTGCTTGC
CGAAGGGTCCCTCGATGAGGTCAAGCGGGACGACGCTGTGGTTGAGAGCTACCTGGGGAGGTAG

Upstream 100 bases:

>100_bases
GGCTGTTCTTCCTGGGGCTGATCTTCGTGCTGGTGCCGTTGCTGTTGCCGAACGGGATCATGGGATTGCTCGAACTCGTC
GCACGCAAGAGGAACCGCAA

Downstream 100 bases:

>100_bases
CTGTTGGGTGGTTGGCAAGTAAGGCCAGCAAAATGGCCGCCTGCCGCAAAAAGGCAAAAGGAACCGACATGCTTGAAGTA
GCCAACCTGAACCAGTACTA

Product: urea ABC transporter ATP-binding protein UrtD

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNENPMVPDLALPEEHDGRALSGVATMGRVVTPGEIDVSHGAILYLEDVTVSFDGFRALNALSLSIDVGELRCIIGPNGA
GKTTMMDVITGKTAPDSGKVFLGQTIDLTRMNEPSIARAGIGRKFQKPTVFEQHPVWENLELAMKTDKGWMASLRARLDR
SAQARIEETLSLIRLEGEALRPAGELSHGQKQRLEIGMLLMQQPALVLLDEPAAGMTDDETMELASLLNRLRGSCSMMVV
EHDMEFVAALAGETGKVTVMAEGRVLAEGSLDEVKRDDAVVESYLGR

Sequences:

>Translated_287_residues
MNENPMVPDLALPEEHDGRALSGVATMGRVVTPGEIDVSHGAILYLEDVTVSFDGFRALNALSLSIDVGELRCIIGPNGA
GKTTMMDVITGKTAPDSGKVFLGQTIDLTRMNEPSIARAGIGRKFQKPTVFEQHPVWENLELAMKTDKGWMASLRARLDR
SAQARIEETLSLIRLEGEALRPAGELSHGQKQRLEIGMLLMQQPALVLLDEPAAGMTDDETMELASLLNRLRGSCSMMVV
EHDMEFVAALAGETGKVTVMAEGRVLAEGSLDEVKRDDAVVESYLGR
>Mature_287_residues
MNENPMVPDLALPEEHDGRALSGVATMGRVVTPGEIDVSHGAILYLEDVTVSFDGFRALNALSLSIDVGELRCIIGPNGA
GKTTMMDVITGKTAPDSGKVFLGQTIDLTRMNEPSIARAGIGRKFQKPTVFEQHPVWENLELAMKTDKGWMASLRARLDR
SAQARIEETLSLIRLEGEALRPAGELSHGQKQRLEIGMLLMQQPALVLLDEPAAGMTDDETMELASLLNRLRGSCSMMVV
EHDMEFVAALAGETGKVTVMAEGRVLAEGSLDEVKRDDAVVESYLGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI150417984, Length=245, Percent_Identity=33.0612244897959, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI27881501, Length=220, Percent_Identity=26.3636363636364, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI30795238, Length=220, Percent_Identity=26.3636363636364, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI31657092, Length=248, Percent_Identity=27.0161290322581, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI27436953, Length=265, Percent_Identity=25.2830188679245, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI47078218, Length=220, Percent_Identity=28.1818181818182, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI45446740, Length=220, Percent_Identity=28.1818181818182, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI6005701, Length=223, Percent_Identity=26.457399103139, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI27262624, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI27262626, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116734710, Length=220, Percent_Identity=30, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27477115, Length=228, Percent_Identity=25.4385964912281, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI153792144, Length=224, Percent_Identity=25.4464285714286, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21536376, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI105990541, Length=213, Percent_Identity=25.3521126760563, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI21536378, Length=220, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789864, Length=253, Percent_Identity=33.596837944664, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790739, Length=236, Percent_Identity=31.7796610169492, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87081791, Length=236, Percent_Identity=29.6610169491525, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789593, Length=249, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790525, Length=232, Percent_Identity=28.0172413793103, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787370, Length=238, Percent_Identity=28.1512605042017, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790190, Length=249, Percent_Identity=28.1124497991968, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788761, Length=238, Percent_Identity=28.9915966386555, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081709, Length=245, Percent_Identity=26.9387755102041, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87082268, Length=246, Percent_Identity=29.6747967479675, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790467, Length=249, Percent_Identity=29.3172690763052, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=28.0851063829787, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788472, Length=227, Percent_Identity=26.8722466960352, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788210, Length=246, Percent_Identity=26.0162601626016, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786253, Length=249, Percent_Identity=26.5060240963855, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787089, Length=229, Percent_Identity=27.5109170305677, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081782, Length=249, Percent_Identity=26.1044176706827, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081834, Length=218, Percent_Identity=30.2752293577982, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786398, Length=240, Percent_Identity=27.0833333333333, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788897, Length=242, Percent_Identity=27.6859504132231, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082267, Length=250, Percent_Identity=28.8, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789672, Length=238, Percent_Identity=25.6302521008403, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787792, Length=246, Percent_Identity=25.609756097561, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786345, Length=246, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790162, Length=216, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789991, Length=214, Percent_Identity=27.1028037383178, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787972, Length=241, Percent_Identity=28.2157676348548, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367384, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790544, Length=242, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789586, Length=246, Percent_Identity=27.2357723577236, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787029, Length=235, Percent_Identity=25.531914893617, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786872, Length=235, Percent_Identity=25.531914893617, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI48994943, Length=241, Percent_Identity=27.8008298755187, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786563, Length=237, Percent_Identity=26.5822784810127, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786803, Length=232, Percent_Identity=28.0172413793103, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789873, Length=211, Percent_Identity=25.1184834123223, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=26.1061946902655, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786319, Length=163, Percent_Identity=31.2883435582822, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788450, Length=252, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788053, Length=174, Percent_Identity=30.4597701149425, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787164, Length=230, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787105, Length=216, Percent_Identity=26.8518518518519, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787712, Length=225, Percent_Identity=27.1111111111111, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787758, Length=248, Percent_Identity=24.5967741935484, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788002, Length=250, Percent_Identity=24, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786698, Length=208, Percent_Identity=25.9615384615385, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787500, Length=230, Percent_Identity=24.7826086956522, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI48994997, Length=247, Percent_Identity=27.5303643724696, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=214, Percent_Identity=32.2429906542056, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510237, Length=243, Percent_Identity=27.9835390946502, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533608, Length=219, Percent_Identity=27.3972602739726, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565938, Length=251, Percent_Identity=26.6932270916335, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=223, Percent_Identity=25.1121076233184, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=267, Percent_Identity=24.7191011235955, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=267, Percent_Identity=24.7191011235955, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193208177, Length=224, Percent_Identity=24.5535714285714, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71996809, Length=267, Percent_Identity=24.3445692883895, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28574150, Length=219, Percent_Identity=29.6803652968037, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI116007328, Length=219, Percent_Identity=29.6803652968037, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI116007184, Length=224, Percent_Identity=24.5535714285714, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221500365, Length=224, Percent_Identity=24.5535714285714, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643674, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221512771, Length=219, Percent_Identity=23.7442922374429, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666092, Length=269, Percent_Identity=23.7918215613383, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920532, Length=224, Percent_Identity=28.125, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580930, Length=224, Percent_Identity=28.125, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581191, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24581189, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24581193, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24581195, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85724858, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85724856, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580547, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580551, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580549, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580545, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574533, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31115; Mature: 31115

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENPMVPDLALPEEHDGRALSGVATMGRVVTPGEIDVSHGAILYLEDVTVSFDGFRALN
CCCCCCCCCCCCCCCCCCCEEHHHHHHCEEECCCCEEECCCEEEEEEEEEEECCCHHEEE
ALSLSIDVGELRCIIGPNGAGKTTMMDVITGKTAPDSGKVFLGQTIDLTRMNEPSIARAG
EEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECEEEEEECCCCCHHHHC
IGRKFQKPTVFEQHPVWENLELAMKTDKGWMASLRARLDRSAQARIEETLSLIRLEGEAL
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCC
RPAGELSHGQKQRLEIGMLLMQQPALVLLDEPAAGMTDDETMELASLLNRLRGSCSMMVV
CCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
EHDMEFVAALAGETGKVTVMAEGRVLAEGSLDEVKRDDAVVESYLGR
EHHHHHHHHHCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNENPMVPDLALPEEHDGRALSGVATMGRVVTPGEIDVSHGAILYLEDVTVSFDGFRALN
CCCCCCCCCCCCCCCCCCCEEHHHHHHCEEECCCCEEECCCEEEEEEEEEEECCCHHEEE
ALSLSIDVGELRCIIGPNGAGKTTMMDVITGKTAPDSGKVFLGQTIDLTRMNEPSIARAG
EEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECEEEEEECCCCCHHHHC
IGRKFQKPTVFEQHPVWENLELAMKTDKGWMASLRARLDRSAQARIEETLSLIRLEGEAL
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCC
RPAGELSHGQKQRLEIGMLLMQQPALVLLDEPAAGMTDDETMELASLLNRLRGSCSMMVV
CCCHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
EHDMEFVAALAGETGKVTVMAEGRVLAEGSLDEVKRDDAVVESYLGR
EHHHHHHHHHCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]