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The map label for this gene is cysD [H]

Identifier: 186476983

GI number: 186476983

Start: 2504851

End: 2505813

Strand: Reverse

Name: cysD [H]

Synonym: Bphy_2232

Alternate gene names: 186476983

Gene position: 2505813-2504851 (Counterclockwise)

Preceding gene: 186476984

Following gene: 186476982

Centisome position: 72.02

GC content: 62.41

Gene sequence:

>963_bases
ATGAGCACGACGCTCGATTCGAATCTCAACGCACCGCTGTCCGTGACGGCGAACCGGATGGATCACCTCGACTGGCTTGA
GGCCGAGTCGATTCACATCCTGCGCGAACTCGTCGCCGAATGCAGCAAGCCCGCGCTGCTGTTTTCGGGCGGCAAGGACT
CCGTCGTCGTGCTGGCGCTCGCGTTGAAGGCATTCGGCCTCGGCGCGAACCGCAAGACGCAGCTGCCGTTCCCGCTCGTG
CACATCGACACGGGTCACAACTACGATGAAGTGATCGACTTCCGCGATCGCCGCGCGCAAGAGATCGGTGCGCAACTGGT
GGTCGGTCACGTCGAAGATTCGATCAAGCGCGGCACCGTGCGTCTGCGTCGCGAAACGGACTCGCGCAACGCGGCGCAAG
CCGTCACGCTGCTCGAAACTATCGAGCAATACGGCTACACGGCGATGATCGGCGGTGCGCGCCGCGACGAAGAAAAGGCG
CGCGCGAAAGAGCGCATCTTTTCGTTCCGCGACGAGTTCGGCCAGTGGGATCCAAAGGCGCAGCGCCCGGAACTGTGGAG
CCTCTATAACGCGCGTCTGCATCAGGGCGAGCATCTGCGCGTGTTCCCGATCTCGAACTGGACGGAACTCGATGTGTGGC
AATACATCGCCCGCGAGAACCTCGAACTGCCGTCCATCTACTACGCGCACAAGCGCGAGATCGTACGCCGCAACGGCCTG
CTCGTGCCCGTGACGCCGCTCACGCCGATCCGCGAAGGCGAGTCGAGCGAACAGGCGCTGGTGCGCTTCCGCACGGTGGG
CGATATCAGTTGCACGTGCCCGGTTGAAAGCGATGCGGACGATGTCGAAAAGATCATCGCCGAAACGGCCGTGACGGAAA
TCACGGAACGTGGCGCGACGCGCATGGACGACCAGACGTCGGAAGCAGCGATGGAACAGCGCAAGAAGCAAGGTTATTTC
TAA

Upstream 100 bases:

>100_bases
ATCCCCACGCCGACGACGCAGATTTCGATTGAATCTTTACGTGTCGCATGCATCGGGTGAGAGCCCGACCATGCGACACG
AACCAACGAAGGACCCAAAC

Downstream 100 bases:

>100_bases
AGAGCACGCAGAGGAAAGAGTCAACATGAGCCGCATTGAGCAACCCGCGCATCAACCTGAAGACCTCGGCGTGCTTCGTT
TCATCACGGCAGGCAGCGTC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTTLDSNLNAPLSVTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLALALKAFGLGANRKTQLPFPLV
HIDTGHNYDEVIDFRDRRAQEIGAQLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEQYGYTAMIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHQGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHKREIVRRNGL
LVPVTPLTPIREGESSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF

Sequences:

>Translated_320_residues
MSTTLDSNLNAPLSVTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLALALKAFGLGANRKTQLPFPLV
HIDTGHNYDEVIDFRDRRAQEIGAQLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEQYGYTAMIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHQGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHKREIVRRNGL
LVPVTPLTPIREGESSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF
>Mature_319_residues
STTLDSNLNAPLSVTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLALALKAFGLGANRKTQLPFPLVH
IDTGHNYDEVIDFRDRRAQEIGAQLVVGHVEDSIKRGTVRLRRETDSRNAAQAVTLLETIEQYGYTAMIGGARRDEEKAR
AKERIFSFRDEFGQWDPKAQRPELWSLYNARLHQGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHKREIVRRNGLL
VPVTPLTPIREGESSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQTSEAAMEQRKKQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=45.3947368421053, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36294; Mature: 36163

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLDSNLNAPLSVTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLAL
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
ALKAFGLGANRKTQLPFPLVHIDTGHNYDEVIDFRDRRAQEIGAQLVVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCE
RLRRETDSRNAAQAVTLLETIEQYGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHQGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHKREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPIREGESSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQTSEAAMEQRKKQGYF
CCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLDSNLNAPLSVTANRMDHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLAL
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
ALKAFGLGANRKTQLPFPLVHIDTGHNYDEVIDFRDRRAQEIGAQLVVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCE
RLRRETDSRNAAQAVTLLETIEQYGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHQGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHKREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPIREGESSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQTSEAAMEQRKKQGYF
CCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]