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The map label for this gene is otsA [H]

Identifier: 186476896

GI number: 186476896

Start: 2407201

End: 2408622

Strand: Reverse

Name: otsA [H]

Synonym: Bphy_2145

Alternate gene names: 186476896

Gene position: 2408622-2407201 (Counterclockwise)

Preceding gene: 186476897

Following gene: 186476888

Centisome position: 69.23

GC content: 63.85

Gene sequence:

>1422_bases
ATGAGCAGATTGATCGTCGTTTCAAACCGCGTCGCGCCGACTCAGGAAGGGCGCCCCGCAGCGGGCGGTCTCGCAATCGG
AGTGCTGGATGCACTGAAGGAAACGGGAGGTGTGTGGTTCGGATGGAGTGGCGAGATCGCCGCCGAGCCTTCGACGCCCG
TCATCGAGAAGCAGGGCAATGTGACCTACGCCACCGTGGGACTGACCAAGCGCGATTACGACCAGTACTACCGCGGTTTC
TCGAATGCGACGCTGTGGCCGACTTTCCATTATCGCAACGACCTGTCGCGCTACGACCGGCAGGAATACGCGGGGTATCT
GCGGGTGAACGTGACGCTCGCGAAGCAGCTCAAGGAACTGATCAGGCCCGACGACATCATCTGGGTGCACGACTATCACC
TGCTGCCGTTCGCGCGCTGCCTGCGCGATCTCGGCGTGAAAAATCCGATCGGCTTTTTCCTGCATATTCCGTTTCCCGTG
CCGGAAGTGATGCGCACCGTGCCGCCGCACGAAGAACTCGTGAAATGCATGTGCAGCTATGACGTGGTCGGTTTCCAGAC
GGATGCGGACCGGCAATCGTTCGTCGATTACATCGAGCGCGGCAATCACGGCACATCGAGCGACGACAACATGATTCACG
CGTACGGCCGCTTCCTGAAGGTGGCCGCCTATCCGATCGGCATCTATCCCGATGCGATCGCGAAAGCCGCCGAGCAGTTC
AGCGACCGGAAGCAGGTGCGCAACATGCGCGACGCGATGCGGGGCCGCAAGCTGATCATGAGCGTCGACCGGCTCGACTA
TTCGAAGGGGCTGGTCGAGCGTTTCCAGGCGTTCGAGCGCCTGCTGCAGAATGCGCCCGGCTGGCATGGCCGCGTGTCGC
TCGTGCAGATCGCGCCGCCGACGCGCGCCGACGTGCAGACCTACCAGCGCATTCGTCAGAACCTCGAAGGCGAAGCGGGG
CGGATCAACGGCCGGTTTGCGCAGCTCGACTGGACGCCGATTCAGTACCTGAACCGCAAATACGAGCGCAATCTGCTGAT
GGCGCTGTTCCGCCAGTCGCAGGTCGGCTATGTGACGCCGCTGCGCGACGGCATGAATCTCGTCGCGAAGGAATATGTCG
CGTCGCAGGATCCCGCGGATCCCGGCGTGCTCGTGCTGTCGCAGTTCGCGGGCGCGGCCGAGCAGCTGCCCGGCGCGCTG
GTCGTGAATCCGTTCGATCTGTCGCAGATGGCCGAGGCGCTGGAGCGCGCGCTGTCGATGCCGCTCGCCGAGCGCCAGGC
GCGGCACGCGGACATGATGGCGCCGCTGCGCGAGAACAATCTGTCGGTGTGGCGCGATACGTTCCTCGCCGACCTGCGCC
ATGTCGCGACGGCTTCGTCGGTGACGGAGAAGGCCGTCAAGCGCGCTGACGTGACGGCATGA

Upstream 100 bases:

>100_bases
ACAATTTTATTCCGCGCCTTGCGATAAAACGGCATGGGGCTTGCGTGCCGTAAGGAAAGTATCACCGCATGTACAGTCAA
CTGAATGAGGTGGAGCCGCA

Downstream 100 bases:

>100_bases
ACTAGTACGTTTCAACAAGCACAGGCGTCACCGGCGGCAGCCCATCGAGGCTGCCGTTTTCGTTTCCAATCCGCCCGGGC
GACGGCCTCAGGCCTGCTGC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGEIAAEPSTPVIEKQGNVTYATVGLTKRDYDQYYRGF
SNATLWPTFHYRNDLSRYDRQEYAGYLRVNVTLAKQLKELIRPDDIIWVHDYHLLPFARCLRDLGVKNPIGFFLHIPFPV
PEVMRTVPPHEELVKCMCSYDVVGFQTDADRQSFVDYIERGNHGTSSDDNMIHAYGRFLKVAAYPIGIYPDAIAKAAEQF
SDRKQVRNMRDAMRGRKLIMSVDRLDYSKGLVERFQAFERLLQNAPGWHGRVSLVQIAPPTRADVQTYQRIRQNLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGAL
VVNPFDLSQMAEALERALSMPLAERQARHADMMAPLRENNLSVWRDTFLADLRHVATASSVTEKAVKRADVTA

Sequences:

>Translated_473_residues
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGEIAAEPSTPVIEKQGNVTYATVGLTKRDYDQYYRGF
SNATLWPTFHYRNDLSRYDRQEYAGYLRVNVTLAKQLKELIRPDDIIWVHDYHLLPFARCLRDLGVKNPIGFFLHIPFPV
PEVMRTVPPHEELVKCMCSYDVVGFQTDADRQSFVDYIERGNHGTSSDDNMIHAYGRFLKVAAYPIGIYPDAIAKAAEQF
SDRKQVRNMRDAMRGRKLIMSVDRLDYSKGLVERFQAFERLLQNAPGWHGRVSLVQIAPPTRADVQTYQRIRQNLEGEAG
RINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGAL
VVNPFDLSQMAEALERALSMPLAERQARHADMMAPLRENNLSVWRDTFLADLRHVATASSVTEKAVKRADVTA
>Mature_472_residues
SRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGEIAAEPSTPVIEKQGNVTYATVGLTKRDYDQYYRGFS
NATLWPTFHYRNDLSRYDRQEYAGYLRVNVTLAKQLKELIRPDDIIWVHDYHLLPFARCLRDLGVKNPIGFFLHIPFPVP
EVMRTVPPHEELVKCMCSYDVVGFQTDADRQSFVDYIERGNHGTSSDDNMIHAYGRFLKVAAYPIGIYPDAIAKAAEQFS
DRKQVRNMRDAMRGRKLIMSVDRLDYSKGLVERFQAFERLLQNAPGWHGRVSLVQIAPPTRADVQTYQRIRQNLEGEAGR
INGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTPLRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALV
VNPFDLSQMAEALERALSMPLAERQARHADMMAPLRENNLSVWRDTFLADLRHVATASSVTEKAVKRADVTA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=469, Percent_Identity=52.6652452025586, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI115533558, Length=365, Percent_Identity=32.3287671232877, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI115533560, Length=365, Percent_Identity=32.3287671232877, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71987012, Length=439, Percent_Identity=29.1571753986333, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319602, Length=469, Percent_Identity=34.1151385927505, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6320279, Length=415, Percent_Identity=28.433734939759, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323537, Length=434, Percent_Identity=27.4193548387097, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323917, Length=382, Percent_Identity=27.2251308900524, Blast_Score=159, Evalue=6e-40,
Organism=Drosophila melanogaster, GI19920676, Length=484, Percent_Identity=35.9504132231405, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53395; Mature: 53263

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGEIAAEPSTPVIEKQGN
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEECCCC
VTYATVGLTKRDYDQYYRGFSNATLWPTFHYRNDLSRYDRQEYAGYLRVNVTLAKQLKEL
EEEEEECCCHHHHHHHHCCCCCCEECEEHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHH
IRPDDIIWVHDYHLLPFARCLRDLGVKNPIGFFLHIPFPVPEVMRTVPPHEELVKCMCSY
HCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHC
DVVGFQTDADRQSFVDYIERGNHGTSSDDNMIHAYGRFLKVAAYPIGIYPDAIAKAAEQF
CCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SDRKQVRNMRDAMRGRKLIMSVDRLDYSKGLVERFQAFERLLQNAPGWHGRVSLVQIAPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRADVQTYQRIRQNLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTP
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
LRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALVVNPFDLSQMAEALERALSM
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCC
PLAERQARHADMMAPLRENNLSVWRDTFLADLRHVATASSVTEKAVKRADVTA
CHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRLIVVSNRVAPTQEGRPAAGGLAIGVLDALKETGGVWFGWSGEIAAEPSTPVIEKQGN
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEECCCC
VTYATVGLTKRDYDQYYRGFSNATLWPTFHYRNDLSRYDRQEYAGYLRVNVTLAKQLKEL
EEEEEECCCHHHHHHHHCCCCCCEECEEHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHH
IRPDDIIWVHDYHLLPFARCLRDLGVKNPIGFFLHIPFPVPEVMRTVPPHEELVKCMCSY
HCCCCEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHC
DVVGFQTDADRQSFVDYIERGNHGTSSDDNMIHAYGRFLKVAAYPIGIYPDAIAKAAEQF
CCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SDRKQVRNMRDAMRGRKLIMSVDRLDYSKGLVERFQAFERLLQNAPGWHGRVSLVQIAPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
TRADVQTYQRIRQNLEGEAGRINGRFAQLDWTPIQYLNRKYERNLLMALFRQSQVGYVTP
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
LRDGMNLVAKEYVASQDPADPGVLVLSQFAGAAEQLPGALVVNPFDLSQMAEALERALSM
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCC
PLAERQARHADMMAPLRENNLSVWRDTFLADLRHVATASSVTEKAVKRADVTA
CHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA