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The map label for this gene is tnpR [H]

Identifier: 186476883

GI number: 186476883

Start: 2394894

End: 2395490

Strand: Reverse

Name: tnpR [H]

Synonym: Bphy_2132

Alternate gene names: 186476883

Gene position: 2395490-2394894 (Counterclockwise)

Preceding gene: 186476884

Following gene: 186476882

Centisome position: 68.85

GC content: 58.46

Gene sequence:

>597_bases
ATGAGCCGCACCTTCGCATACGCCCGTGTTAGTCGTACCGAACTCACCACCAGCAACCAGCTTGAGGAAATCAAAGCGGC
AGGCTTCACGGTCGATTCCAAGCGGATCGTTGAGGAAACCATTAGCGGCAGCACAGAAGCCTTCAAGCGGCCCCTGTTCG
CCAAGCTGGTTGATCGTCTGGAGGCTGGCGATGTGTTGATCGTTACCAAGCTCGATAGGCTTGGACGGTCAGCACTTGAT
GTCCGCTCCACCGTCGAGAAACTGGCGGGCATGGGCGTTCGTGTTCATTGCCTTCAGCTCGGCGGGGCGGACCTCACCAG
TTCAGCAGGGAAGCTGATTATGGGCGTTCTCAGCAGCATGGCTGAGTTCGAACGGGACTTGATCATCGAACGTACACAGG
CAGGATTGGCCCGTGCGAAGGCTGAAGGTACGAAGCTGGGGAGGAAGTCTGCCCTGAGTCCTCAGGAGCAACAGAAGGTC
CGCGAGGCCCTTGCGGGAGGCGTCTCGGTGTCACAGTTGGCAAGGGACTACAGCACGTCACGCGCTACGATCCTGAGGGC
CAAGAGTGCGCTGCGGCCGGAGTTGCTTAAATTTTAG

Upstream 100 bases:

>100_bases
TTAACGAACAGTGTGCGGAACAGTGTGCGAAATATAGCTTGCGTTCGTTTCGAGCAGGCAATATGATTCGTACATCCTTT
TCGCACAGTCTGAGTCCACC

Downstream 100 bases:

>100_bases
GCATTGATAAAGGATAAAGCCCGTTGGAGGGGCGCCCTTTTCGATAGGCGGAGGGGCTTTCCTGACATGCCCCTGAGGTT
TCCGGTAGTTCTAAAGTCCT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSRTFAYARVSRTELTTSNQLEEIKAAGFTVDSKRIVEETISGSTEAFKRPLFAKLVDRLEAGDVLIVTKLDRLGRSALD
VRSTVEKLAGMGVRVHCLQLGGADLTSSAGKLIMGVLSSMAEFERDLIIERTQAGLARAKAEGTKLGRKSALSPQEQQKV
REALAGGVSVSQLARDYSTSRATILRAKSALRPELLKF

Sequences:

>Translated_198_residues
MSRTFAYARVSRTELTTSNQLEEIKAAGFTVDSKRIVEETISGSTEAFKRPLFAKLVDRLEAGDVLIVTKLDRLGRSALD
VRSTVEKLAGMGVRVHCLQLGGADLTSSAGKLIMGVLSSMAEFERDLIIERTQAGLARAKAEGTKLGRKSALSPQEQQKV
REALAGGVSVSQLARDYSTSRATILRAKSALRPELLKF
>Mature_197_residues
SRTFAYARVSRTELTTSNQLEEIKAAGFTVDSKRIVEETISGSTEAFKRPLFAKLVDRLEAGDVLIVTKLDRLGRSALDV
RSTVEKLAGMGVRVHCLQLGGADLTSSAGKLIMGVLSSMAEFERDLIIERTQAGLARAKAEGTKLGRKSALSPQEQQKVR
EALAGGVSVSQLARDYSTSRATILRAKSALRPELLKF

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787638, Length=196, Percent_Identity=53.0612244897959, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1787827, Length=196, Percent_Identity=52.5510204081633, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1787404, Length=182, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21469; Mature: 21338

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTFAYARVSRTELTTSNQLEEIKAAGFTVDSKRIVEETISGSTEAFKRPLFAKLVDRL
CCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCHHHHHHHHHH
EAGDVLIVTKLDRLGRSALDVRSTVEKLAGMGVRVHCLQLGGADLTSSAGKLIMGVLSSM
CCCCEEEEECHHHHCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLIIERTQAGLARAKAEGTKLGRKSALSPQEQQKVREALAGGVSVSQLARDYSTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
RATILRAKSALRPELLKF
HHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
SRTFAYARVSRTELTTSNQLEEIKAAGFTVDSKRIVEETISGSTEAFKRPLFAKLVDRL
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCHHHHHHHHHH
EAGDVLIVTKLDRLGRSALDVRSTVEKLAGMGVRVHCLQLGGADLTSSAGKLIMGVLSSM
CCCCEEEEECHHHHCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLIIERTQAGLARAKAEGTKLGRKSALSPQEQQKVREALAGGVSVSQLARDYSTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
RATILRAKSALRPELLKF
HHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]